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The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish were assayed in this study. The sperm malformation rate in mice was also investigated.

摘 要:分别以环磷酰胺和氟化钠作为阳性诱变剂,以普通棉的棉籽粉及棉籽油作为对照物,同时设阴性对照组,以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料,将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼,检测小鼠骨髓嗜多染红细胞(Polychromatic erythrocytes, PCE)微核率(Micronucleus frequency, MN‰)和精子畸变率,以及斑马鱼外周血红细胞微核率。

The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafishwere assayed in this study. The sperm malformation rate in mice was also investigated.

分别以环磷酰胺和氟化钠作为阳性诱变剂,以普通棉的棉籽粉及棉籽油作为对照物,同时设阴性对照组,以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料,将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼,检测小鼠骨髓嗜多染红细胞(Polychromatic erythrocytes, PCE)微核率(Micronucleus frequency,MN‰)和精子畸变率,以及斑马鱼外周血红细胞微核率。

objective inquire into the value of eet and cag in the diagnose of coronary heart disease.methods review and analyze132cases of positive eet and77cases of results of cag.results positive cag:58cases(75%);negative cag:19cases(25%);single-branch pathology18cases;double-branch pathologies:40cases;many-branch pathologies:19cases;stc extension:25cases;s-wave deepeness:21cases;u-wave inverˉsion:2cases.conclusion among the patients of eet,dangerous factors conform to the results of cag inclinical symptom relatively,among females,young people and non-clinical symptom,false positiveness is more.

目的 探讨活动平板运动试验与冠状动脉造影在冠心病诊断中的价值。方法对132例活动平板运动试验阳性,77例冠状动脉造影结果进行回顾性分析。结果冠状动脉造影阳性58例(75%),阴性19例(25%),单支病变18例,双支病变40例,多支病变19例,stc延长25例,s波加深21例,u波倒置2例。结论在活动平板运动试验患者中,有危险因素和临床症状与冠状动脉造影结果较相符,女性、年轻人和无临床症状者假阳性较多。

The result was consistent with that of double-disk synergy tests. 61 strains were found to be ESBLs negative detected using VITEK GNS-506. Among them, 19 strains were found to be ESBLs positive.

检测102株大肠埃希菌中,仪器检出41株阳性,纸片协同法对照也为阳性,但仪器检测的61株阴性菌中,纸片协同法对照有19株为阳性。

End of internal carotid artery, anterior cerebral artery, posterior cerebral artery, basal artery, and vertebral artery.

以磁共振血管成像为标准,比较经颅多普勒超声检查的特异性,敏感性,假阳性,假阴性及符合率。

He was treated empirically with acyclovir, and multiple blood and CSF cultures were negative.

他在接受经验性的无环鸟苷治疗后,多次血培养和脑脊液培养是阴性。

Results After transfected for 48 boors, endostatin was identified in the cell plasma of infected LLC and negative result was founded in non-infected LLC.

结果 Ad-mES转染LLC48h后,LLC细胞浆内可见棕黄色mES阳性表达颗粒,培养上清可表达位于相对分子质量20000的mES阳性条带,而未转染对照组呈阴性。

The size of an appendix, appendolith, periappendiceal and cecal apical changes were observed and noted.

结果 68例中 61例CT诊断为急性阑尾炎,7例假阴性,CT诊断急性阑尾炎的敏感性为 89.7%。

All patients were T-cell crossmatch negative and received immunosuppression with tacrolimus.

所有的患者T细胞交叉配型阴性,采用tacrolimus进行免疫抑制。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样,利用PCR和SOE技术,获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因,以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗:二基因融合(pCDNA3.1-MPB64-Ag85B,简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6,简称pCMAE)DNA疫苗;二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗,免疫BALB/c小鼠,以牛分枝杆菌BCG免疫组为阳性对照,以pCDNA3.1及PBS免疫组为阴性对照,共免疫3次,每次间隔2周,BCG组仅初免时皮下免疫1次。1免后每周,以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原,以间接ELISA方法检测血清IgG水平及lgG亚类。

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