阴性的

The advanced expression of Filaggrin may start the mechanism of apoptosis ahead of tune and the premature expression of TGK may accelerate the formation of cornified cell membrane,which will speed up the terminal differentiation of keratinocytes.Consequently,the hyperproliferative keratinocytes differentiate and exfoliate.

K17在扁平庆皮损中染色为阴性，而在寻常庆、尖锐湿虎皮损中为阳性，这提示：扁平庆的异常增生比寻常庆、尖锐湿庆要弱，这与扁平庆的临床表现和病理改变相一致；K17与炎尖锐湿疣初期表现症的发生有关，在三种庆的病理改变中，扁平庆的炎症现象最微弱，这与K17的染色阴性相吻合。

He type of TGF β1 expression was related with lymph node metastasis and prognosis of patients. The positivity rate of TGF β1 expression only in mesenchyma in the group on lymph node metastasis was significantly lower than that in no lymph node metastasis, but the positivity rate of TGF β1 expression only in cytoplasma was higher. The prognosis of TGF β1 expression only in mesenchyma was better, but the prognosis of TGF β1 expression only in cytoplasma or both mesenchyma and cytoplasma was poor.

GF β1的阳性表达类型与肺癌的淋巴结转移及预后有关，淋巴结转移阳性组TGF β1的单一间质阳性表达率显著低于淋巴结阴性组，而单一胞浆阳性表达率高于淋巴结阴性组；半年内死亡组的TGF β1单一间质阳性表达率低于5年以上生存组，而间质胞浆均阳性的表达率及单一胞浆阳性表达率则高于5年以上生存组(P均＜0.05)。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Tail Length, Tail Moment, Tail DNA Percentage, and correction rate were compared between the improved software and the original one, and epidemiological indices for the improved software were also calculated and analyzed, including sensitivity, specificity, Youden index, crude agreement, adjusted agreement, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio.

比较改进前后的尾长、尾矩、尾DNA百分含量上的差异、改进前后分析正确率差异、以及改进后的软件以不同DNA损伤级别为截断值判定阳性结果的灵敏度、特异度、Youden指数、粗一致性、调整一致性、阳性预测值、阴性预测值、阳性似然比和阴性似然比。

Results:①In HBsAg positive groups,the SMR were 8.85 in men and 12.50 in women,in HBsAg negative groups, the SMR were 2.87 in men and 3.40 in women, There were significant difference among different groups;②the trend of correlation between HBsAg and primary carcinoma of liver were alike in 5 years before and after;③There were not any significant difference observed about the SMR of carcinoma ventriculi and esophagus cancer in different groups.

结果：①HBsAg阳性组的肝癌标化死亡比男、女分别为8.85与12.50，HBsAg阴性组的肝癌SMR分别为2.87与3.47，两组之间差异显著；②HBsAg与肝癌的关系在前后5年的趋势相似；③HBsAg阴性和阳性组的胃癌及食道癌的标化死亡率无统计学差异。

in 2950 subjects,including 1472 hbsag positive patients and 1478 hbsag negative control, all of them were obversved in 10 years and the standardized mortality ratio of primary carcinoma of liver were calculated in each group.results:①in hbsag positive groups,the smr were 8.85 in men and 12.50 in women,in hbsag negative groups, the smr were 2.87 in men and 3.40 in women, there were significant difference among different groups;②the trend of correlation between hbsag and primary carcinoma of liver were alike in 5 years before and after;③there were not any significant difference observed about the smr of carcinoma ventriculi and esophagus cancer in different groups.

采用前瞻性研究，自1996年7月起对观察对象随访10年，其中hbsag阳性者1472例，阴性者1478例，于2006年10月用流行病学方法分析各种标化死亡比等观察结果。结果：①hbsag阳性组的肝癌标化死亡比男、女分别为8.85与12.50，hbsag阴性组的肝癌smr分别为2.87与3.47，两组之间差异显著；②hbsag与肝癌的关系在前后5年的趋势相似；③hbsag阴性和阳性组的胃癌及食道癌的标化死亡率无统计学差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。