阴性的

The resultsof FR3A and FR3 are very near while the positive rate of FR2 was much lower.However,the latter has a good compensation to the former two.Among the 6negative cases of FR3A,three were positive when amplified with FR2.All ofthe samples from 4 cases of T cell lymphomaand 3 cases of reactivehyperproliferationare negative and one out of the 3cases of Hodgkins'lymphomasamples was positive.

FR3A与FR3结果相近，FR2阳性率较低，但6例FR3A阴性的标本中有3例FR2结果阳性，表明二者有较好的互补性。4例T细胞淋巴瘤和3例反应性增生结果均为阴性，3例霍奇金病中有1例阳性。

Results Among 96 cases of CD117-positive GISTs, 94 positive for nestin (in amount of this 70 diffusive positive, 21 moderate positive, 3 local positive), the other two were negative. 4 of the 5 CD117-negative GISTs and 26 of 33 Schwannomas expressed nestin too, only 3 of 15 leiomyosarcomas showed focal positive for nestin. All oesophagus leiomyomas and fibromatosis of intestinal demonstrated negativity for nestin.

结果 96例CD117阳性的GISTs中，94例表达nestin，其中70例弥漫强表达，21例中度阳性，3例局灶阳性，仅2例阴性；5例CD117阴性的GISTs中，4例表达nestin；33例消化道雪旺瘤中26例表达nestin；15例平滑肌肉瘤中3例局灶表达nestin；10例食管平滑肌瘤、6例肠纤维瘤病均为阴性。

PCNA labeling index in H. pylori positive patients was significantly higher than negative patients in all the chronic gastritis (P.01); from normal controls to gastric carcinoma , there are all significant difference in PCNA labeling index(P .05). Expression of Bcl-2 protein In gastratrophia and gastric carcinoma were significant higher than that in normal controls (P.05), and there were significant difference between H.

在慢性胃炎的各组中HP阳性的病人PCNA LI％均高于HP阴性的病人(P.01)，同时各组间PCNA LI％也有显著性差(P.01)：胃癌组和慢性萎缩性胃炎组的Bcl-2蛋白阳性表达率均显著高于正常对照组(P.05)，同时组内HP阳性病人与阴性病人间Bcl-2蛋白表达也均有显著性差异(P.05)。

Results Rigid bronchoscopy showed foreign bodies in the main bronchus or bronchus intermedius of 42 patients, while spiral CT localized airway foreign bodies in the same patients.

结果 硬支气管镜检查发现42例异物在主支气管或支气管叉，胸部CT扫描显示相同位置异物；硬支气管镜诊断为阴性的9例中，胸部CT检查6例为真阴性而3例为假阳性。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/μl.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

OBJECTIVE, To study the direction, route and num- ber of SLCs and their relationship with SLN after subareolar injection of methylene blue and technetium 99m sulfur colloid （^99m Tc-SC）, and to explore the mechanism of sentinel lymph node biopsies produce false negative results in breast cancer patients and the method to improve the detectable rate, METHODS: ^99m Tc-SC was injected preoperatively, and methylene blue was injected by the same subareo-lar route just after anesthetic induction, and then SLNB was carried out according to SLCs in 93 breast cancer patients.

目的：通过乳晕下联合注射^99m Tc-SC和亚甲蓝，研究前哨淋巴通道的行走方向、途径、数量及其与前哨淋巴结之间的关系，以探讨乳腺癌SLNB出现假阴性的机制，并提出提高其检出率、减少假阴性的方法。

A satisfied cytologic smear, improvement of the smear preparation, understanding the particularity of the disease and cytologists's skill in diagnosis are critical to decrease the false-negative rate. It's important that gynecologis do multiple biopsy under vaginoscope to the patients who are suspicious of cancer but negative cytology.

获取满意的宫颈细胞样本、改进标本处理、认识到病灶的特殊性以及加强细胞学医师的学习和提高自身诊断水平是降低TCT假阴性的有效方法，临床医师对症状体征可疑但细胞学阴性的病例采取阴道镜下多点活检可减少假阴性的发生。

Morphologic findings of thymuses from 32 anti-acetylcholine receptor-negatie myasthenia grais patients, 12 with and 20 without antibodies against the muscle-specific kinase, were compared with those from 30 AChR-positie subjects.

Lauriola等研究了32名乙酰胆碱受体抗体阴性的胸腺形态学变化，其中有12例肌肉特异性激酶抗体阳性，20例阴性；对照组为30例乙酰胆碱受体抗体阳性的病人。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。