阴性

The characteristics of CaBP-LI positive rubrospinal neurons in the red nucleus showed that these neurons was not homogeneous. Rubrospinal neurons had their subpopulation in term of CaBP like immunoreactivity (from strongly posotive to negative) and special distribution features.Key words: calbindin-D28k; immunocytochemistry; red nucleus; rubrospinal tract; rat

大鼠红核-脊髓投射成年人神经元的CaBP-LI阳性和CaBP-LI阴性两种亚型及其分布特点说明，这些成年人神经元的性质并非均一，并可能与红核-脊髓投射成年人神经元对钙超载具有不同的耐受性有关。

Results The rubrospinal neurons were retrogradely identified by cholera toxin subuint B coulpled with colloidal gold (CB-Au,prepared by our laboratory).No CaBP-LI positive neurons identified by CB-Au was present in the rostral level of the red nucleus.

结果 红核-脊髓投射成年人神经元表现为两种亚型：一种为CaBP-LI阴性成年人神经元；另一种为CaBP-LI阳性成年人神经元。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

The negative expression of bFGF in the fetal skin may be one of the important reasons for fetal scarless healing.

bFGF的阴性表达可能是胎儿皮肤无瘢痕愈合的重要原因之一。。

All the LOH on 〓 and 〓 was observed in invasive ductal carcino- ma, carcinoma simplex, medulary carcinoma and scirrhous carcino- ma, no deletions at these sites were observed in any invasive lobular carcinoma and others. These results imply an etiological difference.P53 gene is a hot point gene in the occurrence and development of breast, cancer. PCR-SSCP analysis was performed to detect P53 gene point mutation in the region between exon 5 and 8, 5 of 12 (41. 6%) stage I breast cancer patients contain mutation of P53, 3 of 5 patients were accompanied by 〓 deletion. These results suggested that point mutation and allelic loss of P53 gene are two vi- tal genetic events in earlier stage of breast tumorigenesis.

我们还发现，〓和〓位点LOH均分布在乳腺浸润性导管癌、单纯癌、髓样癌及硬癌中，在浸润性小叶癌和某些特殊类型乳腺癌中全部为LOH阴性，上述位点LOH可能与某些组织学类型乳腺癌的发生有关。P53基因是乳腺癌形成过程中的一个热点基因，本研究对12例Ⅰ期乳腺癌组织标本中P53基因热点区域第5、6、7、8外显子点突变进行了测定，发现41.6％（5／12）的病人有P53基因一个或多个点突变，其中3例同时伴有〓位点LOH，表明P53基因点突变和等位基因缺失是发生在乳腺癌形成早期的一个重要遗传学事件。

Amikacin and Kita sermonic have the best inhibitory effect on Gram-negative bacilli.

丁胺卡那、庆大霉素对革兰氏阴性杆菌的抑制效果最好，均出现高敏反应。

Objective: To study the clinical significance of liver biopsy in chronic hepatic disease patients with negative serological markers.

目的：了解肝组织中肝炎病毒检测对血清肝炎病毒标志阴性患者病原学诊断的临床意义。

Objective To study the etiology of serologically negative non A～E hepatitis .

目的 对血清学标志阴性的非甲～戊型肝炎进行病原学研究。

The data suggest that the patients with serologically negative non A～E hepatitis have a different etiology and should be tested by PCR to identify the disease.

对血清学标志阴性的非甲～戊型肝炎的病人应该用PCR法进行病原学分型，以明确其诊断。

Objective:To investigate the epidemiologic and clinical features of seronegative liver diseases and guide diagnosis and treatment.

目的：探讨肝炎病毒血清学标志阴性肝病患者流行病学和临床特点，指导临床诊断和治疗。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。