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The most common potential pathogen, coagulase-negative staphylococci, were found on all of the keyboards. Diphtheroids were found on 80% of keyboards, Micrococcus species on 72%, Bacillus species on 64%, nonfermentative Gram-negative rods on 36%, vancomycin-sensitive Enterococcus on 12%, Staphylococcus aureus on 4%, and oxacillin-susceptible S aureus on 4%.

在所有键盘中都发现了最常见的致病菌凝固酶阴性葡萄球菌。80%的键盘带有类白喉菌,72%带有微球菌属,64%带有芽胞杆菌属,36%带有非发酵的革兰氏阴性杆菌,12%带有万古霉素敏感肠球菌,4%带有金黄色葡萄球菌,4%带有甲苯异恶唑青霉素易感链球菌。

The most common microbes causing infections in percutaneously inserted uncuffed catheters are, in order of prevalence, coagulase-negative staphylococci, Staphylococcus aureus, Candida species, and enteric gram-negative bacilli.

根据患病率的高低,引起经皮插入的无气囊导管感染的最常见的微生物排序如下:凝固酶阴性葡萄球菌、金黄色葡萄球菌、念珠菌属菌种和肠道革兰氏阴性细菌。

The sensitivity, specificity, accuracy, positive prevalue, negative prevalue, positive likelihood ratio and negative likelihood ratio of hc2 for high-risk HPV were 100%, 87. 1%, 87. 3%, 8.8%, 100%, 7.7 and 0. 000 , respectively ; and those of gene chips were 78.6%, 91.1%, 90. 9%, 9.9%, 99.7%, 8.8 and 0. 235 respectively.

HC2法检测高危HPV对高度宫颈上皮内瘤样病变HSIL的灵敏度、特异度、准确性、阳性预测值、阴性预测值、阳性似然比和阴性似然比分别为100ˇ、87ˇ1ˇ、87ˇ3ˇ、8ˇ8ˇ、100ˇ、7ˇ7和0ˇ000ˇHPV分型基因芯片法以上各指标分别为78ˇ6ˇ、91ˇ1ˇ、90ˇ9ˇ、9ˇ9ˇ、99ˇ7ˇ、8ˇ8和0ˇ235。

Results Among 12 normal conjunctiva, there were 3 weakly positive, 3 positive and 6 negative; while among 15 pterygial specimens, there were 1 negative, 3 weakly positive, 7 positive and 5 strong positive.

结果 12例正常结膜中,6例为阴性,3例VEGF蛋白染色弱阳性,3例为阳性;15例翼状胬肉组织中,1例VEGF蛋白染色阴性,3例为弱阳性,7例为阳性,5例为强阳性。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P<0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略,结合启动子预测结果,分别构建了8个ACL基因和19个IDH3β基因启动子区重组子,将其转染猪PK15细胞系,并利用荧光素酶双报告基因系统检测了它们的活性,结果表明:在所构建的猪ACL基因5侧翼序列的8个重组子中,除pGL-ACL27和pGL-ACL15外,其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P<0.01),表明它们均具有启动子活性,pGL-ACL919活性最强,到pGL-ACL679活性下降了将近3倍,说明从-919bp到-679bp区域可能存在负的调控位点,从pGL-ACL621到pGL-ACL73活性有小幅度下降,到pGL-ACL27活性降到与阴性对照水平无显著差异,表明维持该启动子的基本活性区域位于-73bp到+77bp之间;IDH3β基因启动子活性开始于pGL-IB82,然后虽然有些波动,但是活性一直下降直到-164bp处,之后活性又开始升高,直到-279bp处活性达到最高,这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间,从-82到-279之间的179bp区域具有最高的启动子活性。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示,抗MOG和抗MBP抗体可以预测更多和更早期的发作,39名血清呈阴性的患者中,9位(23%)复发,而重新发作的时间是45 ± 3.7个月,22名带有抗MOG和抗MBP的患者中,21名(95%)复发,复发时间为 7.5 ± 4.4个月,另外42名患者只有抗MOG,35人(83%)在 14.6 ± 9.6个月之内复发(P 。001,二组相较于血清呈阴性的患者)。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示,抗MOG和抗MBP抗体可以预测更多和更早期的发作,39名血清呈阴性的患者中,9位(23%)复发,而重新发作的时间是45 ± 3.7个月,22名带有抗MOG和抗MBP的患者中,21名(95%)复发,复发时间为 7.5 ± 4.4个月,另外42名患者只有抗MOG,35人(83%)在 14.6 ± 9.6个月之内复发(P 。001,二组相较於血清呈阴性的患者)。

The data were analyzed by chi-square test using spss 11.0 statistical analysis software package.results the sensitivity of ecg was 77.2%, specificity 50.0%, false positive rate 50.0%, false negative rate 22.8%, positive predictive value 62.7% and negative predictive value 66.8%.the positive rate of ecg rose with the increase of number of stenosed coronary vessel.

在选择性冠状动脉造影的639例住院患者中,对常规心电图检查进行chd诊断价值的分析比较。并使用spss 11.0统计软件分析包对数据进行r×c表x2检验。结果ecg检查的敏感性为77.2%,特异性为50.0%,假阳性率为50.0%,假阴性率为22.8%,阳性预测值为62.7%,阴性预测值为66.8%。

OBJECTIVE, To study the direction, route and num- ber of SLCs and their relationship with SLN after subareolar injection of methylene blue and technetium 99m sulfur colloid (^99m Tc-SC), and to explore the mechanism of sentinel lymph node biopsies produce false negative results in breast cancer patients and the method to improve the detectable rate, METHODS: ^99m Tc-SC was injected preoperatively, and methylene blue was injected by the same subareo-lar route just after anesthetic induction, and then SLNB was carried out according to SLCs in 93 breast cancer patients.

目的:通过乳晕下联合注射^99m Tc-SC和亚甲蓝,研究前哨淋巴通道的行走方向、途径、数量及其与前哨淋巴结之间的关系,以探讨乳腺癌SLNB出现假阴性的机制,并提出提高其检出率、减少假阴性的方法。

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