阴性

Pedunculated,two were in close proximity to vessels. Conclusion Multiphasic CT has moderate sensitivity in the

数肿瘤在增强早期显示明显，肿瘤邻近血管、蒂状突出及非高密度病灶是造成假阴性的潜在因素。

PETs were most conspicuous on the arterial and pancreatic phase. In the eight examinations with false-negative findings, four tumors were isodense and two were pedunculated, two were in close proximity to vessels.

PET在动脉期和胰腺实质期显示明显，8例假阴性中呈等密度4例，蒂状突出2例，肿瘤邻近血管2例。

Multiphasic CT has moderate sensitivity in the detection of PETs. Most tumors are more conspicuous on the earlier phases of enhancement. Potential sources of false-negative results include tumors adjacent to vessels, pedunculated morphology, and nonhyperattenuating lesions.

薄层螺旋CT多期扫描对PET的检出敏感性高，多数肿瘤在增强早期显示明显，肿瘤邻近血管、蒂状突出及非高密度病灶是造成假阴性的潜在因素。

Firstly, eleven cases with empyema of the gallbladder, biliary sludge, chronic cholecystitis, polypoid cholesterolosis, papilloma of the gallbladder or pedunculated hepatocellular carcinoma were misdiagnosed as gallbladder carcinoma.

在2例伪阴性例中，1例之癌症只有胆囊粘膜上的轻微变化可见，幷只侵犯少许肌肉层，另1例则为全部胆囊壁都是癌肿瘤，且侵犯至总胆管及淋巴腺。

Physical examination:T38 C, R32/min, P120/min, dyspyoria, Fontanel 1.5×1.5cm2, sunken, orbit sunken, decreasedSkin turgor, dry lip, dry periglottis, pharynx, heart rate 120/min, no arrhythmia, mild dull heart sounds, lungs, mild abdomen swelling, soft abdomen, not palpated liver 1.5cm below ribs, bowel sounds 10～12/min, no high notes,two lower limbs patellar reflex negative

体查：T38 C，R32次/分，P120次/分，烦躁不安，体查不合作，前稳囟1.5×1.5cm2，凹陷，眼眶凹陷，皮肤弹性差，唇干燥，舌粘膜干燥，咽，心率120次/分，率齐，心音稍钝，双肺，腹稍隆起，腹软，肝肋下1.5cm未扪及，肠鸣音10～12次/分，未闻及高调音，双下肢膝腱反射阴性。

Results In RT-PCR analysis of the peripherial blood, 45%(18 out 40) of CRC patients, 10%(2 out 20) of CRP patients were positive for CEA mRNA, respectively. All 40 healthy individuals were negative for CEA mRNA expression. The detection of CEA mRNA was significantly correlated with TNM stage of CRC patients, while there were no significant relationship between the CEA mRNA expression and the malignant extent of cell differentiation.

结果 40例结肠癌患者外周血中CEA mRNA表达的阳性率为45.0%(18/40)，而健康对照组均为阴性，两者比较有显著性差异(P.01)；20例结肠息肉患者中CEA mRNA表达阳性率为10%(2/20)；CEA mRNA阳性表达率与肿瘤分期相关，而与肿瘤细胞分化程度并不相关。

Results In cystitis glandularis tissue AQP1 mainly expressed in the endothelial cells of micrangium,the immunohistochemical positive exponent was 35.947;AQP2 expressed in the cell membrane and periplasm of the mucosa epithelial cells,the immunohistochemical positive exponent was 34.644;and AQP3 did not express.

结果：在腺性膀胱炎组织中AQP1主要表达在微血管内皮细胞，免疫组化阳性指数35.947；AQP2主要表达在炎性膀胱黏膜上皮细胞的胞膜及胞质内，免疫组化阳性指数34.644；AQP3则呈阴性表达。

Results: A 2-AAF/PH model was successfully established to activate the oval cell compartment in rat liver. HOC appeared mainly in periportal areas and could extend into the liver parenchyma. The volume of oval cells was about as big as one forth to one sixth of normal liver cells, with a oval-shaped nucleus and high nuclear/cytoplasm ratio.

结果：以2-乙酰氨基芴加三分之二肝切除术成功建立了大鼠肝卵圆细胞增殖模型，HE染色汇管区可见大量增殖的小圆细胞，并可向肝实质内延伸，免疫组化证实增生细胞OV-6、c-kit、CKl9及AFP染色阳性，而白细胞共同抗原染色阴性。

In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks.

在动物体内实验中，选择清洁级ICR纯系小鼠100只，按体质量随机分为5组：阴性对照组每天腹腔注射生理盐水，其余4组每天腹腔注射120 mg/kg D-半乳糖以建立小鼠衰老模型，同时阳性对照组每日以生理盐水灌胃，L、M、H组分别以每日100、200、400 mg/kg TP灌胃。8周后处死小鼠，获取心肌标本进行组织学研究和生化检测。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。