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In order to do this, we get equal quantities of plasmid pSCA1-M, pcDNAM and pSCA1 transfected vero cells. After 48 hours, we observed the presence of special green fluorescence in cytolymph in both of pSCA1-M and pcDNAM groups. However, this fluorescence is absent in the negative pSCA1 group.

分别取等量的重组质粒pSCA1-M、常规IBV M基因DNA疫苗质粒pcDNAM、空载体pSCA1转染Veto细胞。48h后经间接免疫荧光试验观察发现,pSCA1-M以及pcDNAM转染组细胞浆均可见特异性亮绿色荧光,pSCA1空载体组无荧光为阴性

The HB-J strain could not infect the MT4 cell as indicated by the negative result of P24 antigen examination of the supernatant and the absence of cytopathological change.

但该株病毒的PBMC培养上清液转种于MT4细胞后,培养上清液P24抗原和前病毒DNA始终为阴性且不出现细胞病变。

It was negative for co-culture of Vero cells with upper fluid of iris, ciliary body and retina which were blindly transfered to the third generation. No Cytopathological effects were observed, while the positive contrast group had CPE including such as ballooning degeneration, karyopyknosis ,multinucleated giant cell even cell necrosis.

Vero 细胞分别与虹膜、睫状体、视网膜组织上清液共培养,并盲传三代结果为阴性,未观察到细胞病理学改变,对照组有阳性表现,表现为Vero 细胞气球样变、核固缩、多核巨细胞甚至细胞悬浮死亡。

Result150of patients and infected sheep,Cattle,Swine serum positive rates of DAgS -ELISA were 91.4%,74.6%,and 66.7%re spective-ly. In the main, DAgS -ELISA gain adva ntage over of SAT and RBPT.

结果上述3种试验对健康人、羊、牛、猪共计140份血清检查均为阴性反应,用DAgS-ELISA对布氏菌感染人、羊、牛、猪共计150份血清检查,其阳性率分别为91.4%、74.6%、66.7%及66.7%,从总体而言,检查的特异性及敏感性,以DAgS-ELISA优于SAT及RBPT。

Ermcidin is a new antibacterial peptide recently found from human sweat glands, and DCD-1L is a derivant of Dermcidin which has a broad spectrum of activities against Gram negative and positive bacteria.

CD是最近从人汗腺中发现的抗菌肽,DCD-1L是它的衍生物,能够有效地抗革兰氏阴性细菌和革兰氏阳性细菌,具有广谱的抗菌活性。

The histochemistry staining of cytokeratin, vimentin, alkaline phosphatase were positive and that of α-actin,Ⅷ Ag, fibronectin and desmine were negative.

细胞角蛋白、波形蛋白和碱性磷酸酶染色阳性,α-肌动蛋白、Ⅷ因子相关抗原、纤维连接蛋白和结蛋白染色阴性

A total of 150 mesenchymal neoplasms of the GI tract, abdominal cavity, and retroperitoneum were retrieved from consult and surgical pathology archives, including 54 GISTs (8 KIT-negative; 13 desmin-positive), 17 GI leiomyosarcomas, 11 GI mural leiomyomas, 13 leiomyomas of the muscularis mucosae, 12 gastric schwannomas, 15 inflammatory myofibroblastic tumors, 9 cases of mesenteric desmoid fibromatosis, 10 dedifferentiated liposarcomas, and 9 malignant peripheral nerve sheath tumors.

我们从会诊病例及活检病例中找出150例间叶性肿瘤(来自胃肠道、腹腔和后腹膜),其中包括54例GISTs(8例KIT阴性、13例desmin阳性)、17例GI道平滑肌肉瘤、11例GI道肌壁间平滑肌瘤、13例GI道粘膜肌层平滑肌瘤、12例胃的神经鞘瘤、15例炎性肌纤维母细胞瘤、9例肠系膜韧带样纤维瘤、10例去分化型脂肪肉瘤和9例恶性外周神经鞘膜瘤。

Results EY-TF was a dialyzable substance (pH6.9±0.5) with a relative molecular weight of less than 12000, containing 18 kinds of amino acids. The polypeptide and ribose contents of EY-TF were 1.2mg/ml and 152.94μg/ml respectively. The EY-TF was negative in protein reaction test and showed the maximum ultraviolet absorption at 270nm.

结果 EY-TF是一种可透析、含有18种氨基酸、相对分子质量小于12000的小分子物质,其多肽含量为1.2mg/ml,核糖含量为152.94μg/ml,pH6.9±0.5,蛋白反应阴性,最大紫外吸收光谱在270nm处。

Methods The pretreatment method of sample solutions by diatomite adsorption was optimized.

结果 改进后的方法消除了阴性干扰,重复性好。

The tissue section of transbronchial lung biopsy specimens showed diffusedly infiltrated small lymphocytes and a lymphoepithelial lesion. CD20 was positive and CD3, CD5, CD10, CD21, CD23, bc12, bc16 were negative in immunohistochemical stain.

支气管镜下可见左肺下叶、右肺中叶支气管狭窄,用支气管镜进行透壁肺活检,病理为小淋巴细胞弥漫浸润,可见淋巴上皮病变;免疫组化CD20阳性,CD3,CD5,CD10,CD21,CD23,Bc12和Bc16阴性

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第三章汉藏语&的&字结构的类型划分。