阴性

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

Normal retinal and blepharal tissues were procured for negative control; PBS was adopted as blank control.

用正常视网膜组织和眼睑组织作阴性对照，用PBS作空白对照。

Results No positive expressions of nm23Hl and NF-kBP65 were found in normalgroup; whereas positive expressions were observed in retinoblastoma, blepharal basal cell carcinoma , blepharal squamous cell carcinoma and meibomian gland carcinoma.

结果：nm23H1和NF-kBP65在正常视网膜及眼睑组织细胞中呈阴性表达，在视网膜母细胞瘤、眼睑基底细胞癌、眼睑鳞状细胞癌及睑板腺癌等眼部肿瘤中呈阳性表达。

The results are compared and analyzed.Results Among the 244 patients' bronchial provocation test results,160 are positive （65.57%）,77 are negative （31.56%） and 7 are suspected positive （2.87%）.The 160 positive patients are treated with glucocorticoid and bronchodilatation,and the symptoms are relieved.

结果 244例患者支气管激发试验阳性160例（65.57%），阴性77例（31.56%），可疑阳性7例（2.87%）。160例支气管激发试验阳性患者按支气管哮喘予吸入糖皮质激素及支气管舒张剂治疗，症状缓解。

It ruleth the inferiour part of the Belly, and intestines evento The Arse: this house is a Feminine and Cadent house,unfortunate, as having no aspect to the

在肢体上，第六宫掌管了下腹部，包括内脏，小肠和大肠。这一宫是阴性的，果宫，是不幸的宫，因为与上升无任何相位。

Results There were 35 patients with CAG positive and 37 negative.

结果 CAG阳性患者35例，阴性37例。

The positive rate of CAg and CIC in IgG negative patients was 28.57% and 30.95%, respectively.

IgG阴性患者血清的CAg和CIC阳性率分别为 2 8.57%及30 。95 %。

Methods CRW stereo tactic apparatus and CT were used to define nucleus location and Radonics radiofrequeney instruments were used to lesion different subneucleus of amygdale and the front part of callosal gyrus. All schizophrenic patients were diagnosed by CCMD-3 standards.

方法对32例难治性精神分裂症患者，采用CRW立体定向仪和CT定位，用Radonics毁损仪毁损杏仁核不同亚核团和扣带回前部，采用临床疗效总评量表、简明精神病量表和阳性与阴性症状量表和韦氏智力量表等评定疗效，并进行1年的随访。

Neisseria meningitidis, a capsulated Gram-negative bacterium, is the leading cause of bacterial meningitis in children.

中文摘要脑膜炎双球菌（Neisseria meningitidis， NM）是一种带有荚膜多醣的革兰氏阴性菌，是造成孩童细菌性脑膜炎的主要病因。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。