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Objective To establish a sensitive and speedy multiple polymerase chain reaction technique for methicillin resistant staphylococcus aureus and methicillin resistant coagulase negative staphylococci detection.

目的 应用一种敏感快速的多重聚合酶链反应,检测耐甲氧西林的金黄色葡萄球菌和凝固酶阴性葡萄球菌。

The positive rates of d-test of staphylococcus aureus resistant to erythromcin and sensitive to clindamycin and and negative to coagulase were 64.28% and 47.45% respectively.

对红霉素耐药而克林霉素敏感的金黄色葡萄球菌和凝固酶阴性葡萄球菌中,d-试验阳性者分别为64.28%和47.45%。

In Staphylococcus aureus which are resistant to erythromcin and sensitive to clindamycin and Staphylococcus whose coagulase is negtive, the positive rate of D-test are 65.38% and 46.42% respectively.

红霉素耐药而克林霉素敏感的金黄色葡萄球菌和凝固酶阴性葡萄球菌中,D-试验阳性即对克林霉素具诱导耐药性者分别为65.38%和46.42%。

Results Among 1 439 strains of isolated bacteria,456(31.69% were gram-positive bacteria,and 206(45.18% of which were coagulase negative Staphylococcus epidermidis,92(20.18% were coagulase positive Staphylococcus aureus...

结果1 439株细菌中,革兰阳性菌456株(31.69%),以凝固酶阴性表皮葡萄球菌为多,达206株(45.18%),其次为金黄色葡萄球菌92株(20.18%);两种葡萄球菌的耐药率相似,对万古霉素耐药率均为0。

Results In 585 staphylococcus, there were 406 staphylococcus aureus, which including 387 methicillin sensitive staphylococcus aureus and 19 methicillin resistant staphylococcus aureus. And there were 179 coagulase negative staphylococcus, including 20 methicillin sensitive coagulase negative staphylococcus and 159 methicillin resistant coagulase negative staphylococcus.

结果 585株葡萄球菌中,金黄色葡萄球菌406株,其中甲氧西林敏感的SA387株,甲氧西林耐药的SA19株;凝固酶阴性的葡萄球菌179株,其中甲氧西林敏感的CNS20株,甲氧西林耐药的CNS159株。

This study shows that the duplex real time PCR assay with the primers for to mecA and nuc genes is a valuable tool for rapid and precise identification of MRSA and methicillin resistant coagulase negative staphylococci.

对mecA、nuc基因进行二重实时PCR能准确、快速地鉴定耐甲氧西林的金葡菌和凝固酶阴性的葡萄球菌。

Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中,PCR法检测葡萄球菌凝固酶基因56株为阳性;Slidex Staph Plus与PCR法相对应的56株为阳性;试管法检测与前2种方法相对应的54株为阳性,2株为假阴性;玻片法为强阳性;VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

Any of various gram-negative, coccoid microorganisms of the genus Chlamydia, especially C.

衣原体一种衣原体属革兰氏阴性球菌微生物,尤指鹦鹉热衣原体和''。

Result In 330 specimens of EPS, there were 116 strains of gram-positive gram positive coccus,5 strains of gram-negative bacillus,15 strains of Gardnrella vaginallis,99 strains of ureaplasma urealyticum, different degree of drug resistance to various drugs was found.

结果 330例患者的前列腺液标本中,培养出革兰阳性球菌116株,革兰阴性杆菌5株,阴道加德纳菌15株,培养出解脲支原体99株,并对各种抗菌药物表现出不同程度的耐药。

Results Gram-negative bacilli was the chief pathogen the next were Gram-positive coccus and fungus.

结果 革兰阴性杆菌仍占主导地位,其次是革兰阳性球菌、真菌。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。