阴性

OBJECTIVE To investigate the antiseptic resistance and Sulphonamide Compounds gene qacE△1-sul in multidrug resistance of Gram negative bacteria caused nosocomial infection,which aim to provides the basis to control nosocomial infection better.METHODS Multidrug resistant Gram negative bacteria were isolated from clinical.

目的了解多重耐药革兰阴性杆菌耐药现状和耐消毒剂磺胺复合物基因qacE△1-sul的携带率及其阳性菌株临床分布状况，为多重耐药革兰阴性杆菌医院感染预防控制提供依据。

Results At the dosage of 25 mg/kg,AN could induce higher incidence of fetal resorption and death,and decrease the average fetal body weight and le ngth with significant difference(P＜0.05)when compared with neg ative controls.At the dosage of 35 mg/kg,AN could decrease average vital fet us p er litter with significant difference(P＜0.05),and induce chang es of other indexes with highly significant difference(P＜0.0 1).At the dosage of 25 and 35 mg/kg,AN could induce higher teras rate,maternal t eras rate and malformation rate of vital fetus with significant and/or highly s ignificant difference(P＜0.05 or P＜0.01),resp ectively.There existed a dose-effect or dose-response relationship between abo ve indexes and doses.

结果 25 mg·kg-1组死胎率、吸收胎率升高，胎鼠平均体重、体长、尾长减小，与阴性对照组比较差异有显著性(P＜0.05)；35 mg·kg-1组除窝平均活胎数减少，差异有显著性( P＜0.05)，其余各指标与阴性对照组比较差异有非常显著性(P ＜0.01)；25，35 mg·kg-1组畸胎率、母体畸胎率、活胎畸形率升高，与对照组相比差异有显著性和/或非常显著性(P＜0.05或P ＜0.01)，且有明确的剂量效应或剂量反应关系。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Additionally XBP1 mRNA which functioned as centrally regulating molecule during ER stress was spliced partially,while it was completely spliced in positive control cells and unspliced in H7721.These results gave the proof that blocking expression of GnT-V caused H7721"s ER stress and response was more weak than that caused by DTT. It may be the chronic process.Additionally,previous studies reported that the ds-RNA may activate the kinase PKR which phosphorylated eIF2 α and inhibited synthesis of proteins.In this paper the antisense cDNA of GnT-V was integrated with GnT-V-AS/H7721"s gene and functioned through the binding of antisense RNA and mRNA of GnT-V.Additionally,the integration of GnT-V cDNA in genome also may be a non-specific factor.

对这三种ER stress关键分子的检测发现：和阴性细胞相比较，实验细胞中BIP的表达无论是蛋白水平还是转录水平都有明显的上调，但上调程度都要低于DTT处理的ER stress阳性细胞；XBP-1 mRNA在实验细胞中部分被剪接，在阳性细胞中XBP-1 mRNA完全被剪接，而在阴性细胞中其以非剪接形态存在；此外和DTT处理的ER stress阳性细胞相似，实验细胞中的PERK发生磷酸化，表明ER stress过程中通过磷酸化eIF2α抑制蛋白合成机制的活化，这和芯片所检测到的GnT-V-AS／H7721细胞蛋白合成系统水平下调相一致。

Results 336strains of pathogenic bacteria were separated from the urine sample of2865cases and the detectable rate was11.73%of the336separated strains the number of strains of gram negative bacilli was226,the gram positive cocci is92and the fungi is18.the positive rate of escherichia coli was the highest followed by co-agulase negative staphylococci,klebsiella pneumoniae and blue verditer pseudomonas.highest resistance to penicillins and cephalosporins was obsered.

结果 从2865份尿标本中分离出336株病原菌，检出率为11.73%。其中革兰阴性杆菌226株，革兰阳性球菌92株，真菌18株。阳性率最高的是大肠埃希菌，其次是凝固酶阴性葡萄球菌、克雷伯菌、铜绿假单胞菌。药敏结果对青霉素类、头孢类抗生素的耐药率较高。

The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000～5000〓(1.34eV～1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.

2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中，在1000～5000〓范围发现了激光激发显微荧光，该荧光的发光范围换算为电子伏特标度为1.34eV～1.83eV，荧光的发光中心大约位于2750〓，即1.62eV，发光的半峰高宽约为2000〓或0.25eV；指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光；观察到了碲镉汞外延薄膜中一个新的Raman散射峰，位于750〓位置； 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中，发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰，该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV，通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺，提高了碲镉汞外延薄膜的结构质量所致；通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。

Results The diagnostic sensitivity, specificity, positive/negative predictive value, positive/negative likelihood ratio and Youden's index in rheumatoid arthritis patients were 69.4%, 96.8%, 89.1%/94.1%, 19.28/0.317 and 0.658 respectively.

结果 本法对类风湿关节炎的诊断敏感性为69.4%，特异性为96.8%，阳性预测值为89.1%，阴性预测值为94.1%，阳性似然比为19.28，阴性似然比为0.317，Youden指数为0.658。

Tail Length, Tail Moment, Tail DNA Percentage, and correction rate were compared between the improved software and the original one, and epidemiological indices for the improved software were also calculated and analyzed, including sensitivity, specificity, Youden index, crude agreement, adjusted agreement, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio.

比较改进前后的尾长、尾矩、尾DNA百分含量上的差异、改进前后分析正确率差异、以及改进后的软件以不同DNA损伤级别为截断值判定阳性结果的灵敏度、特异度、Youden指数、粗一致性、调整一致性、阳性预测值、阴性预测值、阳性似然比和阴性似然比。

In the developed world there are a number of options: a process called 'sperm washing', which separates sperm from HIV-causing agents before being used for insemination, is safest for couples where a positive man wants to avoid the risk of infecting his negative female partner or reinfecting his positive partner; artificial insemination is the safest way of conceiving for couples with a positive woman and a negative man.

在发达国家他们会有许多选择：&精子洗涤&，即在受精前将精子与引起HIV的物质分离，这样可使阳性的男性避免传染阴性的女伴，或再传染给阳性配偶，对于这种情况，&清洗精子&是最安全的；人工授精，这对于由阳性女伴和阴性男伴组成的夫妇来说是最安全的生育方式。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。