阴性

The antibodies in plasma were also detected by indirect antiglobulin test. The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

There were only 5 cases negative for SYT-SSX genomic by seminested PCR, which were positive for SYT-SSX mRNA by RT-PCR. Three cases of synovial sarcoma negative for SYT-SSX mRNA were also negative for SYT-SSX genomic DNA. One case of synovial sarcoma was positive for SYT-SSX1 genomic DNA by seminested PCR, which can not be distinguished for SYT-SSX fusion type by RT-PCR.

只有5例SYT-SSX mRNA呈阳性而半巢式PCR SYT-SSX DNA检测呈阴性。3例RT-PCR检测SYT-SSX mRNA呈阴性者半巢式PCR亦为阴性结果。1例RT-PCR方法无法确定融合基因类型的病例SYT-SSX DNA检测为SYT-SSX1型。

Nov.. The main symptoms of the bacterial disease on Vigna angularis were canker of stalk.The strains were straight or curved rods; polar flagellum one; Gram-negative; could infect Vigna angularis、 Glycine max、 Vigna radata、 Vigna umbellate、 Phaseolus vulgaris、 Pisum sativum、 Pisum sativum var.humlie; oxidase negative; nitrate reduction negative; 5% glucose agar mucilage positive; esculin hydrolysis positive; starch negative; milk hydrolysis positive; H_2S positive; acid from L-arabinose, D-mannose, D-galactose, cellobiose, trehalose and fructose; 5%NaCl positive; G+Cmol%-69%.

红小豆溃疡病主要症状为茎部溃疡；各菌株菌体均杆状；极生鞭毛1根；革兰氏染色阴性；人工接种能侵染多种豆科植物；好氧；氧化酶阴性；硝酸盐反应阴性；5％葡萄糖营养琼脂黏液生长阳性；七叶灵水解阳性；淀粉水解阴性；牛奶分解产碱；产生硫化氢；能从阿拉伯糖等产酸；最大耐盐量为5％；G+Cmol％为69％等特征，根据《手册》9th ed相关部分，确定该病由野油菜黄单胞菌苜蓿致病变种Xanthomonas campestris pv.alfalfae(Riker，Jones et Davis 1935)Dye 19781引起。

Morphological and physiological identification showed that NTG-01 was Gram-negative, short rod shape, with flagella around the bacteria, V-P test positive and Methyl red test negative. The bacterium can use glucose as carbon suource and produce gases. No H2S produced on TTS medium. Lysine decarboxylase and orthinine decarboxylase, catalase and gelatine test are positive. Arginine decarboxylase, dehydrolase, oxidase and urase test are negative. Sequencing and alignment of 16S rDNA showed that it shared a 99% homologous sequence with Enterobacter aerogenes.

结果显示菌株NTG-01为细菌，革兰氏染色阴性，短杆状，鞭毛周生，V-P试验阳性，甲基红实验阴性，利用葡萄糖产酸产气，不能在TIS培养基上产生H_2S，赖氨酸和鸟氨酸脱羧酶试验呈阳性，精氨酸脱羧酶和双水解酶试验呈阴性，氧化酶和脲酶试验阴性，接触酶和明胶液化试验阳性，但后者反应迟缓，一周后才观测到结果。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/μl.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验，结果显示，0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu／mL以下的革兰氏阳性短芽孢杆菌的生长；1‰以下的山梨酸钾不能完全抑制5×10~4 cfu／mL革兰氏阴性粗短杆菌的生长，对10~4 cfu／mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu／mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长，能明显的抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株生长，对10~5 cfu／mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu／mL革兰氏阴性螺旋菌菌株的生长；能明显抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株的生长，而20 mmoL EDTA能很明显抑制10~5 cfu／mL肠杆菌菌株的生长。25mg／mL、50 mg／mL、75 mg／mL和100 mg／mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用，但抑制程度不同，抑菌活性有一定的变化。

Results The sensitivity,specificity,positive predictive value,negative predictive value,Youden index,accuracy ...

结果检测血清特异性IgG的灵敏度为100·0%，特异度为97·7%，阳性预测值为97·2%，阴性预测值为100·0%，约登指数为0·977，符合率为98·7%，试验的一致率为98·5%；检测血清中总的特异性Ig灵敏度为97·1%，特异度为95·3%，阳性预测值为94·4%，阴性预测值为97·6%，约登指数为0·925，符合率为96·2%，试验的一致率为97·8%；检测唾液中特异性sIgA的灵敏度为96·2%，特异度为94·5%，阳性预测值为89·3%，阴性预测值为98·1%，约登指数为0·907，符合率为95·1%，试验的一致率为97·5%；检测粪便标本中的特异性sIgA的灵敏度为92·0%，特异度为90·2%，阳性预测值为85·2%，阴性预测值为94·9%，约登指数为0·822，符合率为90·9%，试验的一致率为98·6%，CV值均小于15%。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。