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Using the immunohistochemical technique, nerve cell bodies and fibres immunoreactive to the anti-Met-enkephalin sera were observed in the cerebral ganglia, pedal ganglia, visceral ganglia, inspect ganglia and coat ganglia of Octopus ocellatus. Met-ENK immunoreactivity was also localized in the mouth, esophagus, craw, stomach, stomachic caecus, the epithelium of the intestines, rectum, sialoid gland, liver, branchia, branchia gland, ventricle, branchial heart, kidneys, ovary, fallopian tube, back of mantle, abdomen of mantle, wrist, membrane of wrist, funnel, and immunocytes of Octopus ocellatus.

免疫组织化学定位结果表明:(1)在短蛸的脑神经节、足神经节、脏神经节、视神经节、外套神经节、口球、食道、嗉囊、胃、胃盲囊、肠、直肠、前唾液腺、后唾液腺、肝胰脏、鳃、鳃腺、心室、鳃心、肾静脉腺质附属物、卵巢、输卵管腺、外套膜背面、外套膜腹面、腕、腕间膜、漏斗中检测到呈不同阳性反应的Met-ENK分子。

Results To the model rats,Ginkgo flavone (100,200,400mgkg,ig)has protective effects of the impairement of memory of rats and decrease the number of neurons in the Bregma hippocampus containing β-APP proteins.

结果 银杏黄酮(100、200、400mg/kg,ig),能明显对抗老年性痴呆模型大鼠学习记忆能力的下降,抑制背海马和齿状回内β-APP阳性神经元的生成。

Results To the model rats,Ginkgo flavone (100,200,400mg·kg,ig)has protective effects of the impairement of memory of rats and decrease the number of neurons in the Bregma hippocampus containing β-APP proteins.

结果 银杏黄酮(100、200、400mg/kg,ig),能明显对抗老年性痴呆模型大鼠学习记忆能力的下降,抑制背海马和齿状回内β-APP阳性神经元的生成。

Kg,ighas protective effects of the impairement of memory of rats and decrease the number of neurons in the Bregma hippocampus containing β-APP proteins.

结果 银杏黄酮(100、200、400mg/kg,ig),能明显对抗老年性痴呆模型大鼠学习记忆能力的下降,抑制背海马和齿状回内β-APP阳性神经元的生成。

60Mm anterior and 1. 20mm lateral to bregma and 4. 00mm ventral to the cortical surface . 2 days later, ICH was induced by the standard method. At 7d, animals were sacrificed to investigate distribution of EGFP-expressing or BrdU-labeled cells around hemotoma.

根据以上实验结果,在分离、培养、传代获得NSC的基础上,采用pEGFP转染或BrdU标记体外培养NSC后,将细胞移植入前囟前1.60mm,矢状缝向右1.20mm,深4.00mm的细胞增生活跃的前脑脑室下区。2d后诱导脑出血模型,出血后7d处死动物检测EGFP或BrdU阳性细胞在血肿周围的分布情况。

The result showed that there was no expression in the normal control group and an apparent expression of ERK1/2 and Elk in the asthma group. A rather dense expressing of ERK1/2 was at bronchiole and mucous membrane, sub mucous membrane, smooth muscle, cytoplasm and nuclei of the out layer of the smooth muscle cell and an expression of positive fiber at submucous membrane.

结果发现正常肺内没有发现ERK1/2和Elk的表达,而哮喘时肺内有明显的ERK1/2和Elk表达,ERK1/2较密集表达在小支气管和细支气管的粘膜层、粘膜下层、平滑肌层和平滑肌外层细胞的胞浆和胞核中,也可见粘膜下层有阳性纤维表达。

It was popular in some districts and even it pervaded seriously in a few ones. 101 positive cows were detected in total and 2 strains of type 3 Brucella were isolated.

对101份布鲁氏菌病血清学检测阳性奶牛的牛乳进行细菌分离培养,结果分离出2株牛种生物3型布鲁氏菌。

Results c-met expression was detectable in 63% of the normal tissues and in 90% of the cancerous tissues.

结果:c-met在63%(19/30)的正常大肠组织和90%(27/30)的大肠癌组织中阳性表达。

The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting. The 21kD and 11kD allergens of cashew nut were purified by ion exchange chromatography.

结果 腰果粗提液SDS-PAGE显示有10条蛋白条带,Western-blotting显示对腰果过敏患者的阳性混合血清能与其中2个蛋白条带起反应,分子量分别为21kD和11kD。

Results showed:(1) The number of ChAc-IR neurons (62. 25±1. 45) in the group of visceral pain increased obviously. The positive neurons (46. 38±1. 27) in the group of electroacupuncture and visceral pain were lower than those of VP (p.05). These indicate that there were changes in cholinergic neurons' activity, increasing of ChAc function and turbulence of intestinal motility in rat's ileum following the acute colonitis.

结果发现:(1)内脏痛组ChAc-IR神经元(62.25±1.45)明显增高,电针+内脏痛组阳性神经元(46.38±1.27)明显低于内脏痛组,二组之间存在显著差异(p.05),提示急性结肠炎可致继发性回肠内在胆碱能神经元活动的改变,ChAc活性增高,肠动力紊乱,而电针可抑制这一改变。

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