阳性结果

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Results 336strains of pathogenic bacteria were separated from the urine sample of2865cases and the detectable rate was11.73%of the336separated strains the number of strains of gram negative bacilli was226,the gram positive cocci is92and the fungi is18.the positive rate of escherichia coli was the highest followed by co-agulase negative staphylococci,klebsiella pneumoniae and blue verditer pseudomonas.highest resistance to penicillins and cephalosporins was obsered.

结果 从2865份尿标本中分离出336株病原菌，检出率为11.73%。其中革兰阴性杆菌226株，革兰阳性球菌92株，真菌18株。阳性率最高的是大肠埃希菌，其次是凝固酶阴性葡萄球菌、克雷伯菌、铜绿假单胞菌。药敏结果对青霉素类、头孢类抗生素的耐药率较高。

Results: Paired biopsies were available for 350 patients (207 HBeAg-positive; 143 HBeAg-negative).

结果：207例HBeAg阳性患者和143例HBeAg阴性患者有研究前后配对活检结果。

Carried on the animal experiment anion to the corn peptide beverage, the experimental result indicated that, The corn peptide Corn-peptide beverage enforced the swimining durability of mice and The contents of the lactates and the plasma urea were lower, but the contents of liver glycogens and muscle glycogen were higher. Corn-peptide has anti-fatigue effect

对玉米肽饮料进行动物实验，实验结果表明：玉米肽饮料可明显延长小鼠负重游泳时间，且血乳酸、血清尿素、肝糖元三项生化指标检测结果均为阳性，据此判定其具有缓解体力疲劳功能。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

Methods:1100 of positive sputum specimens smears were heated by magnetic hotplate.The result of microscopic examination was compared with the traditional acid-fast stain smear.

应用电磁炉加热法对1100例阳性痰标本涂片抗酸染色镜检，所得结果与传统的抗酸染色镜检结果进行比较。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

HGF expressed in cartilage cell plasm, and showed positive in muscle plasm.

研究结果：组织学结果显示，9周时实验组组织学修复优于对照组；实验组Ⅱ型胶原表达较对照组有增强的趋势；HGF在软骨细胞胞浆表达，且发现肌肉组织的肌浆中有阳性表达。

The result was consistent with that of double-disk synergy test. Among 102 strains of E.coli detected using VITEK GNS-506, 41 strains were found to be ESBLs positive.

结果 仪器检测 48株肺炎克雷伯菌中有24株ESBLs为阳性，用纸片协同法对照结果一致。

We have results from the ALOFT, ALAY, and ANGEL studies that would suggest it has positive effects on markers of cardiovascular and renal damage in patients with type II diabetes, nephropathy, heart failure, left ventricular hypertrophy, and also the elderly.

我们已经获得ALOFT、ALAY和ANGEL研究的结果，这些结果提示，对于2型糖尿病、肾病、心力衰竭和左室肥厚的患者，以及老年人群，阿利克仑对他们的心血管和肾脏损害的标记物有阳性效应。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。