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After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示,当培养细胞在RANKL和M-CSF联合作用下,TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成,骨片吸收实验显示骨片上出现典型的骨吸收征象;在M-CSF(50ng/ml)协同作用下,RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

Presence/absence test: Based on the presence of characteristic or confirmed colonies, results are calculated according to the number of dilutions performed and expressed as

阳性/阴性实验:基于出现的阳性菌落或确认的菌落,结果可根据稀释液的管数计算,表达如下

Results Of 122 GISTs,118(97%) were positive for c kit.

结果 GIST中c kit的阳性表达率为 97%(118/ 12 2 ),阳性信号定位准确。

PCR. RESULTS: The results showed that Ang2 mRNA and Tie2 mRNA were expressed in proliferating hemangioma, involuting hemangioma and venous vascular malformation, but the expression of Ang2 mRNA and Tie2 mRNA in venous vascular malformation was lower than that in proliferating and involuting hemangioma.

结果:增生期、消退期血管瘤及静脉型血管畸形均有Ang2 mRNA, Tie2 mRNA的阳性信号,但是三者阳性信号强弱有显著性差异

Results: Bax immunoreactivity was detected in 44 out of 72 cases of cholangiocarcinoma and 8 out of 25 cases of normal control, in which bile ducts among lobules in normal parts aside of hepatocellular carcinoma tissues were observed.

结果:在72例CHC中,Bax阳性44例(61.1%);用于对照的25例肝细胞肝癌的癌旁肝组织汇管区胆管上皮Bax阳性8例(32%)。bax在CHC中的表达强于正常对照组(P<0.01),在其中53例高、中分化的CHC的表达强于在19例低分化CHC的表达(P<0.01),在肝内胆管癌与肝外胆管癌的表达无显著差异(P<0.05)。

RESULTS Ouabain-like immunoreactivities were observed abundantly in the inner zone of adrenal cortex, known as the zone reticularis of SD rats and Macaca mulattas; weakly in the posterior pituitary of SD rats, but nothing in the hypothalamus.

结果 在大鼠和恒河猴的肾上腺皮质内侧网状带细胞中可以观察到丰富的哇巴因免疫反应阳性物质,垂体后叶有较弱的哇巴因免疫反应阳性物质,而下丘脑并未发现有哇巴因免疫反应物质存在。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测:利用已经克隆的美国柑桔裂皮类病毒cDNA序列,制备生物素标记的核苷酸探针,采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测,通过胶片曝光显示杂交信号,发现在两种方法的检测结果中阳性材料都有强的反应,而阴性材料无反应或较弱,该方法安全可靠、快速准确,适合大量样品的检测。

After 7 days of induced differentiation with bFGF and EGF, the yield ofβ-TubulinⅢpositive cells and GFAP positive cells was 75% and 33%, respectively.

结果:体外培养的BMSCs传4代后,纯度最高,可达(95.23±3.06)%;其诱导分化7天后,75%的细胞表现为β-TubulinⅢ阳性的神经元样细胞,33%的细胞则为GFAP阳性的胶质细胞。

Results There was an extensive staining with both P2X2 and P2X4 receptors positive neurons and nerve fibers localized in medulla oblongata. It was mainly localization in the nucleus tractus solitarius,Ⅻ nucleus and ventrolateral medulla of medulla oblongata. It also scattered throughout rest of medulla.

结果 P2X2和P2X4嘌呤受体的阳性神经元和阳性纤维在新生鼠和成年鼠的延髓都有广泛表达,主要分布在孤束核、Ⅻ颅神经核和延髓腹外侧区,延髓其他区域为散在的分布。

RESULTS: FISH demonstrated that the male pronuclei in some one-cell embryos and the each nucleus in some two-cell embryos presented positive signals.

结果: 在单细胞胚和二细胞胚间期核内发现HBV DNA阳性杂交信号,RT-PCR可以扩增出HBx基因的特异条带,免疫荧光也可见到HBsAg阳性信号。

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