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Results: In contrast to negative expression in normal oral mucosa, survivin was expressed in 42 of 50 (84%) cases of oral squamous cell carcinoma samples. There was no significant correlation between survivin expression and age and sex. In squamous cell carcinoma tissues, the expression of Survivin gene stepped up following malignant degree, but the positive rate of Survivin was not statistically significant.

结果:Survivin蛋白在10例正常口腔粘膜组织中呈阴性,而在50例口腔鳞癌组织中有42例阳性,占84%,差异有极显著性(P.01);COX-2蛋白在10例正常口腔粘膜组织中呈阴性,但在口腔鳞癌组织中的阳性率为86%(43/50),差异有极显著性(P.01);Survivin蛋白阳性率与年龄和性别无相关性,而与COX-2蛋白表达阳性呈正相关(P.05)。

Results: There 15 cases were pyretic (15.6%), 41 cases were masculine of peritoneum stimulate syndrome (42.7%), 64 cases were abdominal distention (66.7%), 18 cases were abdominal pain (18.8%), 47 cases were masculine of motion sonant (48.9%).

结果:发热15例(15.6%),腹膜刺激征阳性41例(42.7%),腹胀64例(66.7%),腹痛18例(18.8%),移动性浊音阳性47例(48.9%)。

The result show: The strong positive reaction on the plasma membrane of type Aspermatogonia and the head of maturing sperm, and a lesser positive reaction on the plasma membrane round spermatid.

结果表明:(1)在A型精原细胞的质膜和成熟的精子头部有强阳性反应,早期精子细胞质膜有较强的阳性反应。

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上,本研究进一步运用免疫沉淀和Western Blot印迹方法,检测AML患者原代细胞JAK2和STAT3活化情况,并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现,11例AML患者中,有3例呈现STAT3组成型活化(STAT3阳性,下同),无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性;在此3例患者中,1例未取得缓解,另外2例虽然取得短暂缓解,但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高,而凋亡率数值较低。

PAb-DO-1-P53 was used to study the expression of MTS1-P16 and mutant P53 tumor suppresser gene in paraffin embedded tissues of 38 cases of prostate cancer.

结果显示:在高、中、低三种不同分化的前列腺癌组织切片中MTS_1-P_(16)的阳性表达率为46.2%、21.4%和10%:突变P_(53)的阳性表达率为38.5%、71.4%和72.7%。

Results The positive rate of HBV DNA was the highest in patients with chronic hepatitis or symptomless HBV carriers,it was significant higher than that in patients with cirrhosis and acute hepatitis;the highest rate of HBV DNA was present in patients wit...

结果 各病型 HBV DNA阳性率以慢性肝炎和无症状携带者最高,与急性肝炎肝硬变组比较差异显著;HBe Ag阳性病例中 HBV DNA阳性率以无症状携带者、急性肝炎、慢性肝炎组最高,与慢性肝炎、肝硬变组比较差异显著;在抗-HBe阳性病例中,HBV DNA阳性率以慢性肝炎、肝硬变组为高,与急肝、无症状携带者比较差异非常显著。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库;(2)经蓝白菌落筛选和斑点杂交,正向消减文库获得307个阳性克隆,反向消减文库获得78个阳性克隆;(3)正向消减文库挑选55个克隆成功测序,经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95%~100%),这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等;(4)反向消减文库挑选31个克隆成功测序,经同源性检索和比对最终确定差异表达基因片段16个,其中15个与人类全长基因具有很高相似性(95%~100%),包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性,表明它可能是未被克隆的人类基因EST片段。

Results The Bartonella sp.strains could decompose disaccharide without disassociation of glycose,trisaccharide and spirits,and produce bacillosporin with positive of glucose oxidation and ureolysis test,negative of hydrogen sulfide production test and oxidase test....

结果检测到巴尔通体菌株具有分解双糖,不分解单糖、三糖和醇类,葡萄糖氧化阳性,硫化氢试验阴性、尿素分解试验阳性,氧化酶试验阴性,能产生多粘菌素等生化特性。结论全自动微生物鉴定系统能快速检测出巴尔通体一些特殊的生化反应特性。

Results PCNA positive smooth muscle cells appeared 6 hours after transplantation, increased with time, and reached a peak at 1 to 2 weeks. After 2 weeks, PCNA positive SMC in the tunica media began to decline and recovered to the 6 hour level at 8 weeks after the operation.

结果 移植术后6h,吻合口处中膜即出现PCNA阳性平滑肌细胞,并随着时间推移逐渐增高,在1~2周左右达到高峰。2周后,中膜PCNA阳性SMC开始逐渐减少,于术后8周趋于平稳。

Compared with the "three antibodies method", the sensitivity for detection of the signal of Y chromosome fluorescence in situ hybridization in brain tissue sections of mice was enhanced significantly by the direct tyramine signal amplification method.

结果 "三步抗体法"在脑切片检测Y染色体阳性信号的灵敏度和特异度分别为85.67%和100%;直接TSA法在脑切片检测Y染色体阳性信号的灵敏度和特异度分别为92.82%和100%。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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