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Results The positive detecting rate of aerobe bottle against aerobe was 100%, Sensitivity was 10 CFU/ml. The positive detecting of anaerobe bottle against anaerobe was 100%, Sensitivity against peptococcus niger, Peptostreptococcus anerobius, bacteroides fragilis was 10 CFU/ml, 100 CFU/ml, 1000 CFU/ml, respectively.

结果 需氧瓶对需氧菌的阳性检测率为100%,检测敏感性为10 CFU/ml;厌氧瓶对厌氧菌的阳性检测率为100%,对黑色消化球菌、厌氧消化链球菌和脆弱类杆菌的检测敏感性分别为10 CFU/ml、100 CFU/ml和1 000 CFU/ml;厌氧瓶对兼性厌氧菌的阳性检测率为100%,检测敏感性为10 CFU/ml。

Section in situ hybridization demonstrated that the Fstl1 transcripts in cervico-lumbar spinal cord were distributed in the gray matter neuro-cells and ependymal cells. hybridization signals were present widely in dedifferentiated cells and mesenchyme cells at 2 wks and 3 wks while also in cartilaginous cells at 3 wks of regenerating blastema after tail amputation.

原位杂交结果显示,多疣壁虎脊髓中Fstl1的阳性信号主要存在于灰质神经元细胞及室管膜细胞;而在断尾再生2~3周尾芽中,Fstl1阳性信号广泛存在于再生尾芽的新生区未分化细胞及间充质细胞,3周尾芽中,阳性信号还存在于软骨生成区。

RESULTS The blood biochemistry index of the huanghankang treated group is much lower than others; The RPE thickness of the three groups has no difference; Compare to the positive control group, the Bruch membrane thickness of the huanghankang treated group is thinner.

结果 黄斑康治疗组血脂、血液流变学、丙二醛(maleic dialdehyde,MDA)指标明显低于阳性药物对照组和阴性对照组;黄斑康治疗组与阴性对照组、阳性药物对照组比较,色素上皮(retinal pigment epithelium,RPE)厚度无明显差异;黄斑康治疗组与阳性药物对照组比较,玻璃膜厚度变薄;黄斑康治疗组与阴性对照组比较,玻璃膜厚度变薄。

Results The experiment of immunohistochemical double-staining showed there were cardinal red granules in violet cytoplasm of VEGF-C positive TAM, and VEGF-C was weakly positive in intratumoral tissue, and strongly positive in peritumoral tissue.

结果 免疫组织化学双重染色显示:VEGF-C阳性表达的TAM中可见在紫黑色胞浆内有深红色颗粒存在,且VEGF-C在肿瘤内呈弱阳性表达,而在肿瘤周围间质的TAM中表达呈强阳性。

Results: The radioactivity in organs in ANP group was higher than other groups'in experiment 1; In experiment 2, the results of cultures of chyle fluid, pancreas, lymph node from the experimental group were positive, whereas the results of cultures of all organs in ANP group were positive.

结果:实验一结果表明ANP组中各脏器的放射活性明显高于其它组;实验二组中实验组乳糜液、胰腺、淋巴结细菌培养阳性,而ANP组中各脏器细菌培养均阳性。

Some SPR-LI neurons were also positive for TH. After administration of vasopressin, SPR-LI structures became denser, especially at levels of pyramidal decussation and area postrema . The dendrites of motor dorsal nucleus of X in the dorsal part of MVZ appeared thin and straight in morphology instead of curl and thick outlooks. These results implicate that some SPR-LI neurons might receive or regulate the cardiovascular stress information induced by vasopressin.③In PHA-L/FOS/TH triple-labeled experiments, seven kinds of immunohistochemical structures were found in MVZ. They were FOS-LI neurons, TH-LI neurons, PHA-L positive fibers, PHA-L positive fibers terminated on FOS-LI or TH-LI neurons, FOS/TH doublelabeled neurons, PHA-L positive fibers terminated on FOS/TH double-labeled neurons.

在锥体交叉和最后区水平,SPR免疫反应阳性结构较正常组明显增强;背内侧部迷走神经运动背核的SPR纤维也由弯曲的粗纤维变为细的直纤维,30%的SPR阳性神经元为SPR/FOS双标记神经元,提示这些神经元参与感受心血管伤害性刺激信息,继而参与心血管活动信息的中枢传导或整合;③三重标记研究结果显示,在MVZ可见到七种免疫反应阳性结构,即PHA-L,FOS,TH,FOS/TH,PHA-L/FOS,PHA-L/TH,PHA-L/FOS/TH。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

Results EROD activities in liver of wild crucian from Zhangtan, Jiangjunyan, Fuyang and Wenyan (23.51±4.17, 16.79±7.39, 18.74±5.16, 18.65±8.86 nmol/) were significantly higher than that of Jiekou (7.84±2.42 nmol/g promin), and no difference was observed between the other sites and Jiekou. Three dose groups (0.25, 0.50, 1.00L/dish water sample extracts) were used in Ames test. Reversional mutation rate of TA98 colony exposed to extracts from Zhangtan was 2.21, 3.39, 6.95 without S9, and 2.70, 4.07, 6.23 with S9, respectively. The revesional mutation of TA100 colony was 2.55, 2.81, 5.36 without S9, and 1.73, 2.86, 4.14 with S9, respectively. Reversional mutation rate of TA98 colony exposed to 1.00L/dish extracts from Jiangjunyan with S9 was 2.22, and there was a dose-response relationship in reversional colony count. Ames tests of the other extracts were negative.

结果 樟潭、将军岩、富阳、闻堰点位野生鲫鱼肝EROD酶活性分别为(23.51±4.17),(16.79±7.39),(18.74±5.16),(18.65±8.86)nmol/,明显高于街口[(7.84±2.42)nmol/],其他点位与对照比较差异无统计学意义;樟潭水样浓集物在浓度为0.25,0.50,1.00 L/皿时在不加与加S9条件下,TA98回变菌落突变率MR值分别为2.21,3.39,6.95,2.70,4.07,6.23;TA100回变菌落MR值分别为2.55,2.81,5.36,1.73,2.86,4.14;Ames试验结果为明显阳性;将军岩水样浓集物在浓度为1.00 L/皿且加S9条件下TA98MR值为2.22,且在浓度为0.25,0.50,1.00 L/皿加S9条件下TA98回变菌落数呈剂量-反应关系,MR值分别为0.88,1.98,2.22;Ames试验结果为弱阳性;其他点位均为阴性。

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