阳性的

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

Forced by the grammar to take a stand one way or the other, hermaphrodite is male and indecisiveness female.

又因为语法所迫，总得在阴阳性中选一个归属，于是阴阳人是阳性的，而犹豫不决是阴性。

Sensitive range from 49.1% to 100%, specificity range from 18.2% to 90%,accurate range from 45.5% to 88.3%, PPV range from 34.1% to 95.2,NPV range from 22.2% to 100%,the combine sensitive was 82.7%,specificity was 63.3%;there were not obvious publish bias in the 9 articles, and there was heterology of the 9 articles, the forest figure show RR was 2.34[1.43,3.05],and its 95% confidence interval did not include 1,the chance of malignancy nodules'SUV above 2.5 were more than benign nodules.

文献报道的敏感性49.1-100%，特异性18.2-90.0%，准确性45.5-88.3%，阳性预测值34.1-95.2%，阴性预测值22.2-100%，Meta分析合并后的敏感性82.7%，特异性为63.3%，准确性为71.8%，阳性预测值80.8%，阴性预测值57.0%；漏斗图成近似倒三角形，认为文献无明显发表偏移；对文献异质性检验，P小于0.05，认为文献来源存在异质性；选用随机效应模式得到森林图，RR为2.34[1.43，3.05]，其95%的可信区间不包括1，认为肿瘤病人最大SUV 2.5法呈阳性的可能性大于非肿瘤病人，二者之间具有统计学差异；两篇来源于日本的文献显示日本国的18F-FDG PET肺占位显像假阴性率高于我国。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Of 23 cases of definitive septicemia, 22 cases were found positive.

对血培养阳性的23例进行PCR，结果阳性22例。

In 2 dogs with suspect MRA diagnosis, one was found positive and one negative by angiograph.

MRA诊断为可疑阳性的2只犬，血管造影证实1只为阳性，另1只为阴性。

In the developed world there are a number of options: a process called 'sperm washing', which separates sperm from HIV-causing agents before being used for insemination, is safest for couples where a positive man wants to avoid the risk of infecting his negative female partner or reinfecting his positive partner; artificial insemination is the safest way of conceiving for couples with a positive woman and a negative man.

在发达国家他们会有许多选择：&精子洗涤&，即在受精前将精子与引起HIV的物质分离，这样可使阳性的男性避免传染阴性的女伴，或再传染给阳性配偶，对于这种情况，&清洗精子&是最安全的；人工授精，这对于由阳性女伴和阴性男伴组成的夫妇来说是最安全的生育方式。

In the deeloped world there are a number of options: a process called 'sperm washing', which separates sperm from HI-causing agents before being used for insemination, is safest for couples where a positie man wants to aoid the risk of infecting his negatie female partner or reinfecting his positie partner; artificial insemination is the safest way of conceiing for couples with a positie woman and a negatie man.

在发达国家他们会有许多选择：&精子洗涤&，即在受精前将精子与引起HI的物质分离，这样可使阳性的男性避免传染阴性的女伴，或再传染给阳性配偶，对于这种情况，&清洗精子&是最安全的；人工授精，这对于由阳性女伴和阴性男伴组成的夫妇来说是最安全的生育方式。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

The curative effect in PPD strong-positive patients was better than PPD positive patients,and the curative effect in PPD positive patients was better than PPD negative patients.

PPD试验强阳性患者的治疗疗效优于PPD试验阳性的患者，PPD阳性的患者的治疗疗效优于PPD试验阴性的患者。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。