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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

RESULTS: There was plentiful COX2 protein in the cortex, the hippocampus, the dentate gyrus, the supraoptic nucleus, the amygdaloid nucleus, cerebellum and brain stem, and so on.

结果:在正常成年大鼠脑内COX2蛋白主要表达于皮层、海马、齿状回、视上核、杏仁核、小脑和脑干等部位,阳性细胞主要为神经元,神经胶质细胞染色很少。

These results show that there are reciprocal CGRP-positive fiber connections among the MrD of the striatum, amygdaloid nucleus, bed nucleus of the stria terminals and substantia nigra and its dorsolateral area. It is suggested that there is an affinity relationship between the MrD and limbic system.

结果提示:CGRP阳性纤维在纹状体边缘区、杏仁核、终纹床核以及黑质背外侧区之间形成了一个相互联系的神经网络,纹状体边缘区与边缘系统之间存在密切的纤维联系。

Fig.5 Fos-positive neurons in the amygdaloid nuclei.

图5 杏仁核簇内的Fos阳性问答神经元。

RESULTS: There was plentiful COX2 protein in the cortex, the hippocampus, the dentate gyrus, the supraoptic nucleus, the amygdaloid nucleus, cerebellum and brain stem, and so on. The COX2 immunopositive cells were mainly neurons, and a few were glial cells.

结果:在正常成年大鼠脑内COX2蛋白主要表达于皮层、海马、齿状回、视上核、杏仁核、小脑和脑干等部位,阳性细胞主要为神经元,神经胶质细胞染色很少。

RESULTS: Forebrain beta-EP neurons were found mainly in and around the arcuate nuclei of normal rats, with small number of neurons also identified with weak postivity in the cerebral cortex and amygdaloid complex.

结果:正常大鼠前脑β-EP神经元主要分布于弓状核及其周围,在大脑皮层和杏仁复合体也显示了少量β-EP弱阳性神经元。

The third fluroquino is one kind of antibiotics which is often used in ophthalmology. It's antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物,它对革兰氏阴性菌有良好的抗菌活性,但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱,对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

The third fluroquino is one kind of antibiotics which is often used in ophthalmology. Its antibacterial activity for Gram-negative bacteria is better than that for Gram-positive bacteria and it is the weakest for anaerobe and endo-pathogens.

第三代氟喹诺酮类药物是眼科常用的抗菌药物,它对革兰氏阴性菌有良好的抗菌活性,但对革兰氏阳性菌(如肺炎球菌、化脓性链球菌、肠球菌)的抗菌作用相对较弱,对厌氧菌和胞内病原体(衣原体、支原体、军团菌、分枝杆菌)作用较差。

Analogously, parameters of sub-cluster of Chinese herbs are obtained.

本文通过群子统计参数,分析了现今抗艾滋病的高频中药,其结果表明这些高频药有两类:一是有抗艾滋病病毒的阴性药;二是提高免疫力的阳性药;继而从分析中得出特殊单味药的规律,即r:和rZ均大的中药,具有良好的抗艾滋病功能。

There were four fetal deaths and one anencephalus in B19-DNA positive group. There was no fetal deaths or congenital malformation in B19-DNA negative group.

其中在67例B19 DNA阳性者中,发生4例死胎和1例无脑儿,在268例B19 DNA阴性者中,无一例发生死胎和胎儿畸形,阳性与阴性者间比较,差异有显著性(P.05)。

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