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Positive nerve fibers are in pedal nerve cords and visceral nerve cords. There are no neuronal nitric oxide synthase or inducible nitric oxide synthase positive cells or fibers in the nervous system. A few of endothelial nitric oxide synthase strongly positive nerve cells are identified in pedal ganglia and visceral ganglia. Weakly positive cells and nerve fibers of eNOS can be seen in all of the ganglia and nerve cords.

免疫组化定位表明,神经型一氧化氮合酶和诱导型一氧化氮合酶在整个神经系统内均呈阴性;足神经节和脏神经节内有少量神经细胞呈内皮型一氧化氮合酶强阳性;各神经节和神经索内的部分小细胞和神经纤维呈eNOS弱阳性

The expression of integrin β1 and αvβ3 on cultured human retinal pigment epithelial cell after injury were stronger than that after wound healing.

在色素上皮细胞中,视网膜脱离和复位后两种整合素表达都增高,整合素β1变化较为显著,在脱离后7-28天有阳性表达,复位后阳性表达时间缩短。

RESULTS: FISH demonstrated that the male pronuclei in some one-cell embryos and the each nucleus in some two-cell embryos presented positive signals.

结果: 在单细胞胚和二细胞胚间期核内发现HBV DNA阳性杂交信号,RT-PCR可以扩增出HBx基因的特异条带,免疫荧光也可见到HBsAg阳性信号。

FISH revealed that HBV DNA-positive signals were presented in the oocyte chromosomes, female pronuclei of the zygotes and each nucleus of 2-cell embryos.

用最后几次洗液进行斑点杂交未发现HBV DNA阳性信号,排除了PCR和Southern阳性结果来自洗液污染的可能性。

Results PML RAR α fusion gene of 8 onsetting APL patients were all positive (4 first occurrence, 4 reoccurence);6 of 20 were positive in APL remission phase,in which proportion ratio of promyelocyte in bone marrow smear were all below 5%; a new t...

结果 :发病期的 8例APL患者(初发 4例、复发 4例)PML RARα融合基因均阳性;缓解期APL患者的 2 0人次检查中 6人次阳性,而其骨髓涂片早幼粒细胞比率均小于 5 %;发现一种新的S型变异易位,并见L、S型同时存在于同一个体。

Results: Both EGF treatment and 50 Hz MF exposure could induce EGFR clustering; however, nystatin pretreatment disrupted this effect.

结果:阳性对照组和50 Hz磁场处理组均能观察到EGFR的聚簇现象;制霉菌素预处理1 h后,细胞膜上脂筏结构被破坏,阳性对照组和磁场处理组中受体聚簇现象基本消失。

The number of caspase-3-positive cells was nearly zero in the sham-operation group.

结果:①免疫组化结果:caspase-3阳性细胞表达为细胞胞浆着棕黄色,阳性细胞主要分布于血肿周围和大脑皮质的神经元。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

ISH-IHC double staining showed that positive signals of both ISH (purple-blue NBT-BCIP and IHC (redbrown, AEC expressed in the cytoplasms.

原位杂交阳性(紫蓝色,NBT-BCIP)和免疫组化阳性信号同时表达于靶细胞胞浆中而呈紫红色。

The capacity of the primary library was 5.0×105 PFU/mL and the capacity of the amplified library was 8×109 PFU/mL. The cDNA library was immunoscreened by the rabbit serum against H.qinghaiensis larval tick, the positive pluques were subcloned, and their plasmids extracted and then transformed into Escherichia coli JM109. Eventually, 11 genes were obtained and designated HqL1,HqL2,HqL3,HqL4,HqL5,HqL6,HqL7,HqL8,HqL9,HqL10 and HqL11,respectively.

采用常规方法构建了青海血蜱幼蜱cDNA表达文库,初级文库的库容量为5.0×105PFU/mL,扩增后的库容量为8×109PFU/mL;用兔抗青海血蜱幼蜱阳性血清对该文库进行了免疫学筛选,对阳性噬菌体进行了亚克隆,提取质粒后转化宿主菌JM109,最终共得到幼蜱基因11个,分别命名为HqL1、HqL2、HqL3、HqL4、HqL5、HqL6、HqL7、HqL8、HqL9、HqL10、HqL11。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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