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Results: Bax immunoreactivity was detected in 44 out of 72 cases of cholangiocarcinoma and 8 out of 25 cases of normal control, in which bile ducts among lobules in normal parts aside of hepatocellular carcinoma tissues were observed.

结果:在72例CHC中,Bax阳性44例(61.1%);用于对照的25例肝细胞肝癌的癌旁肝组织汇管区胆管上皮Bax阳性8例(32%)。bax在CHC中的表达强于正常对照组(P<0.01),在其中53例高、中分化的CHC的表达强于在19例低分化CHC的表达(P<0.01),在肝内胆管癌与肝外胆管癌的表达无显著差异(P<0.05)。

In the pregnant group, p38MAPKpositive expression was weak at 3 days and peaked at 4 to 5 days in the endometrium of pregnant mice, then declined in the lumina epithelium, but increased in decidual cells.

d呈强阳性表达,此后腔上皮表达逐渐减弱;腺上皮p38MAPK的表达一直维持在阳性水平,而蜕膜细胞的表达从孕5d开始逐渐增强。

With this in mind, the researchers then reanalysed data from knowingly HIV-positive heterosexual subjects in Uganda and Zambia who had HIV-negative sexual partners. Those with a viral load of one million particles per millilitre were more than ten times more likely to accidentally infect their partners each year than those with a load of one thousand.But when the researchers calculated the transmission potential over the entire predicted asymptomatic period, those with viral loads between ten thousand and a hundred thousand copies per millilitre of blood were the most likely to infect their partners. HIV-positive subjects with viral counts of 50,000 infected roughly twice as many people as those with counts of one million.

伦敦帝国学院流行病学家Christophe Fraser和他的同事对收集于1982年到1993年之间的100多例HIV阳性的男性同性恋者的数据进行分析,并结合分析来自乌干达和赞比亚的已知有HIV阴性性伴侣的HIV阳性异性恋受调查者的资料,他们发现中度病毒负荷而无症状的HIV携带者更易播散HIV,这是因为中度病毒负荷的HIV携带者的无症状期较长,而那些高病毒负荷而无症状的HIV携带者虽然传染性稍强,但其传染的持续时间却很短,并且在未经治疗的HIV携带者个体中中度病毒负荷是最常见的人群。

RESULTS Ouabain-like immunoreactivities were observed abundantly in the inner zone of adrenal cortex, known as the zone reticularis of SD rats and Macaca mulattas; weakly in the posterior pituitary of SD rats, but nothing in the hypothalamus.

结果 在大鼠和恒河猴的肾上腺皮质内侧网状带细胞中可以观察到丰富的哇巴因免疫反应阳性物质,垂体后叶有较弱的哇巴因免疫反应阳性物质,而下丘脑并未发现有哇巴因免疫反应物质存在。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测:利用已经克隆的美国柑桔裂皮类病毒cDNA序列,制备生物素标记的核苷酸探针,采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测,通过胶片曝光显示杂交信号,发现在两种方法的检测结果中阳性材料都有强的反应,而阴性材料无反应或较弱,该方法安全可靠、快速准确,适合大量样品的检测。

After 7 days of induced differentiation with bFGF and EGF, the yield ofβ-TubulinⅢpositive cells and GFAP positive cells was 75% and 33%, respectively.

结果:体外培养的BMSCs传4代后,纯度最高,可达(95.23±3.06)%;其诱导分化7天后,75%的细胞表现为β-TubulinⅢ阳性的神经元样细胞,33%的细胞则为GFAP阳性的胶质细胞。

Methods Acute cardiac injury was established by intramyocardial injection of formalin. Fos and GFAP positive response in Nucleus Tractus Solitarii were investigated by immunofluorescence.

向心肌内注射福尔马林造成急性心肌损伤,免疫荧光染色观察NTS中Fos阳性和GFAP阳性细胞的反应情况。

Results There was an extensive staining with both P2X2 and P2X4 receptors positive neurons and nerve fibers localized in medulla oblongata. It was mainly localization in the nucleus tractus solitarius,Ⅻ nucleus and ventrolateral medulla of medulla oblongata. It also scattered throughout rest of medulla.

结果 P2X2和P2X4嘌呤受体的阳性神经元和阳性纤维在新生鼠和成年鼠的延髓都有广泛表达,主要分布在孤束核、Ⅻ颅神经核和延髓腹外侧区,延髓其他区域为散在的分布。

Antibody responses to hepati tis C virus and its modes of transmission in dialysis patients. Nephron, 1991, 5 9: 2328 Pol S, Romeo R, Zins B et al. Hepa titis C virus RNA in anti-HCV p ositive hemodialyzed patients: significance and therapeutic implications.

本组资料中具有输血史的患者在HCV抗体阳性组中所占的比例明显大于在HCV抗体阴性组中所占的比例,似乎支持这一观点,但另一方面,仍有8例HCV抗体阳性患者未曾有过输血史,提示HD患者存在输血以外其它途径感染的可能[3]。

Methods: Cervical swabs taken from 98 sex workers, 261 gynecological physical check-up clients and 599 sexual transmitted infections clinic female outpatients were cultured using commercial selective medium. Some positive cultures were further, biotyped and serotyped by PCR. Cervical or urethral swabs taken from 80 sexual transmitted infections clinic outpatients were cultured.

对98例卖淫妇女、261例妇科体检病人以及599例性病门诊女性患者的宫颈涂片进行解脲脲原体的培养,部分培养阳性的标本用PCR的方法进行Uu生物分群,对生物1群阳性的标本进一步区分血清型,并对该流行病学资料进行了统计学分析。

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