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The immune constitution chemical method was adopted to measure the positive TH neurons , whose cell plasm should be dyed into tan.

常规免疫组化法检测阳性神经元个数,阳性神经元判断标准:细胞浆棕褐色。

The results were confirmed by virus isolation. The RNA positive control in the assay was invitro transcribed dsRNA which was stabler than single strand RNA used in other similar test.

本研究建立的检测方法以体外转录的dsRNA为阳性对照,具有高度的稳定性,解决了类似检测方法中RNA阳性对照易降解的难题。

The results were:① The number of pCREB immunoreactive neuclei and total amount of pCREB in the subnuclei of rat amygdala, Dentate gyrus and Cornu Ammonis 3(CA3) were increased after FS.

在DG颗粒细胞层内,对照组的pCREB阳性细胞核数目很少,成线状散在分布;而FS后pCREB阳性细胞核的数目明显增多,成带状密布于整个DG颗粒细胞层。

Results After transfected for 48 boors, endostatin was identified in the cell plasma of infected LLC and negative result was founded in non-infected LLC.

结果 Ad-mES转染LLC48h后,LLC细胞浆内可见棕黄色mES阳性表达颗粒,培养上清可表达位于相对分子质量20000的mES阳性条带,而未转染对照组呈阴性。

Brooders and filial generation forepart larvaeof penaeid shrimp were detected using nested-PCR for the presence of WSSV. The results showed that 4 hi 7 genealogies showed WSSV positive at 2,2 period and theres one genealogy that brooder and larvae are all showing positive.

通过建立中国对虾家系,利用巢式PCR法跟踪检测中国对虾亲体及后代早期幼体阶段携带白斑综合症病毒(white spot syndrom virus,WSSV)状况,发现7个家系中的4个家系Z_2期即可检测阳性;其中一组亲虾、产出的卵子及各期幼体均检测阳性

Ethods Tissue specimens were stained immunohistochemically for MMP-2 and MMP-9 in 30 cases of squamous cell cervical carcinoma,29 cases of cervical intraepithelial neoplasia and 16 cases of normal cervices.

MP- 2鳞癌组与CIN组及正常组相比差异有极显著性(P 。0 1)。 MMP- 9正常组与鳞癌组及 CIN组相比差异有显著性(P 。0 5 )。MMP- 2在各组间质的基质细胞中表达均为阳性。MMP- 9在正常宫颈组织、CIN及鳞癌的基质细胞中阳性表达分别为 0 / 16 (0 %);4/ 2 9(13.8%);7/ 30 (2 3.3%)。各组间相比差异无显著性。

Expression of TGF- 1 and iNOS mRNA was higher in the control chick retina and choroid than that in FDM. The immunostaining of TGF- 1 and iNOS protein mainly was detected in the outer part of photoreceptor layer, and nonspecific immunostaining was found in the RPE and choroids.

在鸡视网膜和脉络膜组织有TGF-β1和iNOS蛋白质及其mRNA的表达,TGF-β1和iNOS的阳性染色主要分布于视网膜光感受器的外节,并且位于光感受器外节的不同位置,而在RPE及脉络膜无明显的阳性染色。

After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示,当培养细胞在RANKL和M-CSF联合作用下,TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成,骨片吸收实验显示骨片上出现典型的骨吸收征象;在M-CSF(50ng/ml)协同作用下,RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

It is concluded that the positive rate of platelet antibody screening is very high in patients with hematologic malignancies, the coincidence rate of platelet antibody crossmatch in 249 blood samples is between 40% and 48%, and the effeciency of using crossmatched platelets in clinic is enhanced significantly.

微柱凝胶免疫分析技术在血小板抗体筛选中的应用大部分免疫性血小板减少性紫癜患者血小板膜上可测出血小板相关抗体,包括IgG、PA IgM、PA IgA,其中一项出现阳性,即为血小板抗体检测阳性,而且其量往往与外周血的血小板数量呈负相关,因此检测这种血小板相关抗体有助于免疫性血小板减少性紫癜的诊断[1]。

Epithelial cells showing hybridization signal in cytoplasms predominated in the positive cells.

杂交阳性细胞中,胞浆出现阳性杂交信号的细胞占主要部份。

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