阳性

in 2950 subjects,including 1472 hbsag positive patients and 1478 hbsag negative control, all of them were obversved in 10 years and the standardized mortality ratio of primary carcinoma of liver were calculated in each group.results:①in hbsag positive groups,the smr were 8.85 in men and 12.50 in women,in hbsag negative groups, the smr were 2.87 in men and 3.40 in women, there were significant difference among different groups;②the trend of correlation between hbsag and primary carcinoma of liver were alike in 5 years before and after;③there were not any significant difference observed about the smr of carcinoma ventriculi and esophagus cancer in different groups.

采用前瞻性研究，自1996年7月起对观察对象随访10年，其中hbsag阳性者1472例，阴性者1478例，于2006年10月用流行病学方法分析各种标化死亡比等观察结果。结果：①hbsag阳性组的肝癌标化死亡比男、女分别为8.85与12.50，hbsag阴性组的肝癌smr分别为2.87与3.47，两组之间差异显著；②hbsag与肝癌的关系在前后5年的趋势相似；③hbsag阴性和阳性组的胃癌及食道癌的标化死亡率无统计学差异。

It appears in Zhang Linqi, HIV-positive pregnant women, even prior to the delivery, if timely intervention of drugs, AIDS continues to be mother to child transmission probability from 30% to 15%, as the most crucial is to reduce the mother of the AIDS virus in the body, Therefore, early detection, early treatment will bring about the blocking effect of high-quality,"just down the mountain to block HIV, is slowly declining, through medication, at least two weeks to a buffer in order to allow HIV-positive pregnant women in the body The AIDS virus from high to very low levels of inhibition."

在张林琦看来，HIV阳性的孕妇即便到了分娩之前，如若及时进行药物干预，依然能将艾滋病母婴传播几率从30%降低至15%左右，因为最为关键的是降低母亲体内的艾滋病病毒量，故而，早发现、早治疗会带来优质的阻断效果，&阻断艾滋病病毒就跟下山一样，是个慢慢下降的过程，通过服药，至少需要两周时间缓冲，才能让HIV阳性的孕妇体内的艾滋病病毒从很高水平抑制到很低水平&。

Quote pregnancy test first morning urine, such as positive, on the note you are pregnant, and if negative, can go to the hospital for blood serum human chorionic promote gland hormone tests, such as positive, you can clear diagnosis.

引用早孕测清晨第一次小便，如为阳性，就说明您怀孕了，如为阴性，可去医院抽血作血清人绒毛膜促腺激素化验，如为阳性，即可明确诊断。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

On the other hand, we found for the first time that mammalian neurosteroids-like immunopositive nerve cell and its fiber existed in the brain vesicle and nerve tube of Amphioxus, and the positive substance distributed in the cytoplasm of nerve cell, while nucleus showed negative reaction.

本文研究还首次发现，文昌鱼脑泡和神经管中存在哺乳动物神经甾体激素样免疫阳性神经细胞及其纤维，免疫阳性物分布在神经细胞胞质，核显阴性反应。

Results Positive HCV RNA was found in supernatants of B cell line. HCV Ag and HCV RNA were also showed positive. Electron microscopy observed HCV spherical virus like particles with a diameter of approximately 65 nm and 110nm and the "bud mutation"of HCV in the cytoplasmic vesicles of B lymphocytes.

结果细胞系上清液中HCV RNA呈阳性，细胞内HCV抗原及HCV RNA均呈阳性，电镜观察结果发现细胞内存在65nm和110nm圆球型病毒颗粒，并可见病毒芽生形成现象。

In carcinomatous mucosa, immunoreaction of the carinomatous cell nest was negative, or only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.

结果 正常组织的基底膜呈现很强的Ⅳ型胶原抗体阳性反应，在基底膜形成较粗的环形染色带；Ⅲ型胶原抗体阳性反应在细胞外间质呈连续的细丝纤维样。

Result: Determination:α-inhibin positive occurs on cell nucleus or cell plasm, CD99 positive occurs on cell membrance or plasm.

结果判定：α-抑制素阳性在核或浆，CD99阳性表达在细胞膜或浆。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内，在血管和淋巴管内皮细胞则无表达；VEGFR-3亦表现为广泛的表达，于血管和淋巴管内皮细胞可见阳性表达，并随着胚胎的发育，其在淋巴管内皮细胞的阳性表达率明显增加。

Result摘要: Determination摘要:α-inhibin positive occurs on cell nucleus or cell plasm, CD99 positive occurs on cell membrance or plasm.

结果判定摘要：α-抑制素阳性在核或浆，CD99阳性表达在细胞膜或浆。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。