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All the study subjects had known colonic disease (a history of colorectal cancer or adenomatous polyps, ulcerative colitis, or positive radiographic findings) or were suspected to have it based on signs and symptoms such as rectal bleeding, hematochezia, melena, or positive fecal occult blood test.

所有受试者都患有结肠疾病(结直肠癌,腺样息肉,溃疡性结肠炎或影像学阳性发现),或根据症状和体征(如直肠出血,便血,黑便,或者大便潜血阳性),疑似患有上述疾病。

Results: the number of th positive cells and the viability of mesencephalic cells significantly decreased while the number of inos cells increased in the development of apoptosis induced by mpp+.

结果:在mpp+诱导的中脑原代细胞凋亡中,mpp+组th阳性细胞数及细胞存活率均显著低于各egb761组及阳性对照组(p.01)。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞,且随培养时间的延长而增多。2周时,软骨组织甲苯胺蓝染色呈强的红色异染反应,Ⅱ型胶原免疫组织化学染色呈强阳性,番红&O&染色呈强阳性

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型,胞质丰富,胞核成圆形或椭圆型,核仁清楚,甲苯胺蓝呈异染性,阿尔新蓝8Gx 染色阳性,细胞外基质Ⅱ型胶原免疫组化染色阳性,Ⅰ型胶原染色阴性。

Rats 20 days after birth the TGF-βexpression in the metaphysis:positive signals in cells,dark blue,located in cytoplasm;positive expression intensity of the use of quantitative analysis of optical density.

仔鼠生后20天时TGF-β在干骺端的表达:阳性信号的细胞呈深蓝色,定位于细胞浆;阳性表达强度采用平均光度进行定量分析。

Methods To collect 10 specimen residual ear cartilage of microtia patients about 8-year-old and cultured in vitro, dye with toluidine blue and immunohistochemistry of collagen type II.

结果:残耳软骨细胞在体外培养时随代数增加呈异染陛的糖胺多糖减少,第1、3代Ⅱ型胶原阳性细胞比例约68%~71%,第5代为23%、显著降低;冻存后第3代为65%,与同代数的细胞没有差别,100ng/mi浓度的CDMP-1可以将第4代细胞的阳性比例维持在66%,而且冻存和生长因子不会引起细胞周期的变化。

Methods The nitroglycerin experimental migrainous animal model was reconstructed for the expression of c-fos and c-jun by means of immunohistochemical method Results The nitroglycerin experimental migrainous rats appeared reddish two ears, head shaking,scratching head with forepaws, activity increasing, both the number increasing and the area enlarging of the positive cells of the expression of c-...

免疫组化ABC法研究偏头痛大鼠脑组织即刻早期基因c -fos、c -jun的表达。结果硝酸甘油型实验性偏头痛大鼠出现双耳发红、甩头、前肢频繁搔头,活动增加等外在表现,脑组织c -fos、c -jun基因表达阳性细胞数增加,基因表达阳性细胞的面积扩大、灰度降低或变化不大。结论硝酸甘油型实验偏头痛大鼠模型复制方法简单、重复性较好、脑组织c -fos和c -jun基因异常表达明显,可以作为偏头痛治疗药物筛选、药效评价和发病机理研究的动物模型。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7%),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3% and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌,其中革兰阴性杆菌225株(78.4%),革兰阳性球菌41株(14.3%),念珠菌21株(7.3%),检出菌来自呼吸道标本占63.4%,其他标本各占5%左右;细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%;耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%;大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%,铜绿假单胞菌对亚胺培南的耐药率为40.2%;白色念珠菌对氟康唑的耐药率为81.3%,对两性霉素的耐药率为3.2%。

The percentage of different organ involvements in these patients is 60% of nephropathy, 37% of arthritis, 48% of mucocutaneous lesions, 11% of CNS involvements, and 38% of fever.

关节炎则在37%的病人上呈现,而皮肤炎及黏膜侵犯则可在48%的病人身上发现,中枢神经侵犯则有11%,另外有38%的病人一开始会以发烧来表现。实验室数据的异常,包含在所有病人中有93.4%病人抗核抗体呈阳性反应,而抗双股去氧核糖核酸抗体制则有81.9%为阳性

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果: NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

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