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PAb-DO-1-P53 was used to study the expression of MTS1-P16 and mutant P53 tumor suppresser gene in paraffin embedded tissues of 38 cases of prostate cancer.

结果显示:在高、中、低三种不同分化的前列腺癌组织切片中MTS_1-P_(16)的阳性表达率为46.2%、21.4%和10%:突变P_(53)的阳性表达率为38.5%、71.4%和72.7%。

Results The positive rate of HBV DNA was the highest in patients with chronic hepatitis or symptomless HBV carriers,it was significant higher than that in patients with cirrhosis and acute hepatitis;the highest rate of HBV DNA was present in patients wit...

结果 各病型 HBV DNA阳性率以慢性肝炎和无症状携带者最高,与急性肝炎肝硬变组比较差异显著;HBe Ag阳性病例中 HBV DNA阳性率以无症状携带者、急性肝炎、慢性肝炎组最高,与慢性肝炎、肝硬变组比较差异显著;在抗-HBe阳性病例中,HBV DNA阳性率以慢性肝炎、肝硬变组为高,与急肝、无症状携带者比较差异非常显著。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库;(2)经蓝白菌落筛选和斑点杂交,正向消减文库获得307个阳性克隆,反向消减文库获得78个阳性克隆;(3)正向消减文库挑选55个克隆成功测序,经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95%~100%),这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等;(4)反向消减文库挑选31个克隆成功测序,经同源性检索和比对最终确定差异表达基因片段16个,其中15个与人类全长基因具有很高相似性(95%~100%),包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性,表明它可能是未被克隆的人类基因EST片段。

But the latent period for reaching the platform above the water in Morris Water Maze of the Mn-exposed group was not different from that of control group. There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

In hybridization in situ sections, there were no iNOS positive cells in FO group, iNOS positive cells were found in other 3 groups in mucous layer , tela submucosa and vascular wall.

原位杂交DAB染色:假手术组均未见到iNOS阳性细胞,其他组胃粘膜全层均可见到阳性细胞,包括粘膜层及粘膜下层的细胞、血管壁。

There was a 81.3% positive predictive value in patients with strongly positive tet.

平板运动试验强阳性诊断冠心病的阳性预测值为81.3%。

The effect of thymus environment on T cell development and tolerance.

在所挑选的这些抗体中,并不是所有对细胞系阳性的抗体对正常的细胞也呈阳性反应,说明培养的细胞可能表达了一些正常细胞不表达的抗原。

Toluidine blue staining and typeⅡcollagen immunohistochemistry staining also showed positive results in both groups, but they were more significant in the experimental group.

石蜡切片HE染色可见类似正常软骨的组织学特征,甲苯胺蓝染色及Ⅱ型胶原免疫组化染色阳性,实验组的阳性染色程度较高。

Results The results of 3 methods were identical,totally 22 positive samples in 38 throat swabs were observed in the study,the positive rate is 57.9%.

结果三种方法分别检测38份咽拭子标本,阳性均为22份,阳性检出率均为57.9%,检测结果完全一致。

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