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Results showed that β-catenin was positive expression in stellate reticulum,inner dental epithelium,and dental sac at cup stage,in inner dental epithelium,dental papilla cell,and cell nucleus at early bell stage,in cervical loop and cusp cell membrane at late bell stage,and in ameloblast and odontoblast cell at amelogenesis stage.

结果表明,帽状期在星网层、内釉上皮、牙囊均为细胞核阳性表达;钟状早期在内釉上皮、牙乳头细胞核阳性表达;钟状晚期在颈环、牙尖细胞膜阳性表达;成釉期早期在成釉质细胞、成牙本质细胞阳性表达;成釉期晚期均阴性表达。

Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中,PCR法检测葡萄球菌凝固酶基因56株为阳性;Slidex Staph Plus与PCR法相对应的56株为阳性;试管法检测与前2种方法相对应的54株为阳性,2株为假阴性;玻片法为强阳性;VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

Hybridization signals were identified in the nuclei of tumor cells and the positive cells distributed diffusively or focally. Tag expression was found in 75 human brain tumor tissues out of 96 SV40 DNA positive cases. Tag-RB specific complex was found in all of 75 Tag positive cases.

SV40 DNA定位于肿瘤细胞核,阳性细胞呈弥漫或片灶状分布。96例SV40 DNA阳性脑瘤组织Tag表达阳性75例,所有Tag表达阳性瘤组织均发现Tag与RB形成特异性复合物。

Results The megakaryocyte and platelet of 112 cases hemopathy marrow smear with acetal phospholipids staining were all positive, the plasmas cell in anaemia were positive, the erythroblast of aplastic anemia positive, the lipocyte in thrombocytopenic purpura positive, and the other cells all negative.

结果]112例血液病骨髓涂片缩醛磷脂染色中的巨核细胞和血小板均为阳性,贫血中的浆细胞为阳性,再生障碍性贫血中的幼红细胞为阳性,血小板减少性紫癜中的脂肪细胞为阳性,其余各种细胞均呈阴性反应。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株,没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA,作PCR检测。D1代获得5株阳性转化植株,分别是90035的4株,85—593的1株,转化率为3.8%。6、将D1代获得5株阳性转化植株的种子在温箱中发芽,提取DNA检测,得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

Results Among 12 normal conjunctiva, there were 3 weakly positive, 3 positive and 6 negative; while among 15 pterygial specimens, there were 1 negative, 3 weakly positive, 7 positive and 5 strong positive.

结果 12例正常结膜中,6例为阴性,3例VEGF蛋白染色弱阳性,3例为阳性;15例翼状胬肉组织中,1例VEGF蛋白染色阴性,3例为弱阳性,7例为阳性,5例为强阳性

Results: The number of BrdU-positive cells in the subventricular zone and in the subgranular zone of dentate gyrus,and the numbers of ChAT-positive cells in medial septumand diagonal band of the basal forebrain were significantly decreased in lead exposed group P

结果:铅中毒大鼠齿状回的颗粒下层、侧脑室室管膜下区的BrdU阳性细胞数、基底前脑内侧隔核和斜角带核的ChAT阳性细胞数以及大脑皮质和海马齿状回NF阳性神经元数、嗅球BrdU+NF阳性细胞数目以及学习记忆能力明显下降,GFAP阳性细胞反常升高P

Results HPV DNA was positive in 26 cases of specimens(26/100),and as four basic macroscopic types HPV was positive in 5 of 25 cases of ulcerating type esophageal carcinomas,in 3 of 25 cases of fungating t...

结果 共检测出HPV阳性 2 6例(2 6 / 10 0 ),按照食管癌的病理大体分型,其中溃疡型食管癌中HPV阳性 5例(5 / 2 5 )、蕈伞型中阳性 3例(3/ 2 5 )、缩窄型中阳性 12例(12 / 2 5 )、髓质型中阳性 6例(6 / 2 5 )。

Results The morphology of endocrine cells in digestive tract was of multiplicity.The density of 5-HT cells was the highest in the colon,but not found in the esophagus,cardia gastric and cecum.The results showed that SS cells were distributed in the pylorus mostly,but not detected in the cardia,colon,cecum and rectum.Gas positive cells were the most in the duodenums,while in the esophagus and cardia was not discovered.In the cardia VIP cells were maximum,but in the esophagus,duodenums and ileum did not appear.

结果 消化道中4种免疫阳性细胞形态多样,大多呈椭圆形和锥体形。5-HT阳性细胞数量以结肠最多,空肠和回肠次之,幽门腺区、十二指肠和直肠较少,食管、贲门腺区、胃底腺区和盲肠中未见;SS阳性细胞在幽门腺区数量最多,贲门腺区、结肠、盲肠和直肠中未见;Gas阳性细胞大量出现于十二指肠,在食管、贲门腺区和盲肠未见;除个别器官未见VIP阳性细胞外,在消化道的其余各段均有分布。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%),稳定转染得到的阳性克隆最多,且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少(约20d);用阳离子聚合物瞬时转染率低(1.7%),稳定筛选同样可以得到阳性大克隆,但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多(约30d);阳离子脂质体转染效果居中,瞬时转染率为39.9%,从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

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