阳性

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性，进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠，3次免疫小鼠后，血清(1：1000)中能检测到较强的MUC1抗体阳性；以最大OD450值之纯化血清做为一抗行蛋白印迹分析，结果显示：纯化血清可识别MUC1蛋白；小鼠脾脏淋巴细胞增殖率检测结果表明：噬菌体展示肽表位可在无佐剂存在情况下，大大促进小鼠的脾淋巴细胞的增殖，证明噬菌体是一较好的生物载体。

Results ① FGF8 was hardly detected in the whole embryos at E8.75d, but at E9.5d, FGF8 mRNA expression was found at the most anterior border of prosencephalon, isthmus ...

结果①在E8·75d整个鼠胚几乎没有FGF8的表达；到E9·5d、E10·5d，在前脑泡的前缘、脑峡部位和神经管的末端观察到较局限的紫蓝色阳性区域，但到E10·5d时，上述3个部位的染色较E9·5d减弱；E11·5d时阳性信号主要集中在中脑部位。

Results Trunk neural crest stem cells were successfully isolated and cultured. The immuocytochemcal result showed that the cells were nestin-and P75-positive. Trunk neural crest stem cells grew through adhering to chitosan fiber after inoculation. Under an electon microscope, the spindle-shaped trunk neural crest stem cells were proliferated and migrated along the fiber. The cells arranged side by side or linked by heads and tails in processes. The ends of S-100 positive cells were flat and expanded, adhering to the fiber as claw-shaped pseudopodium.

结果 采用神经管植块法培养的神经嵴干细胞，免疫细胞化学染色呈现nestin及P75双阳性，将其接种在壳聚糖纤维支架材料上，可见神经嵴干细胞贴附于壳聚糖纤维上生长；S-100免疫细胞化学染色显示，S-100阳性细胞突起的末端呈扁平状膨大；扫描电镜显示，壳聚糖纤维上的躯干神经嵴干细胞为梭形，呈现"端对端"、"肩并肩"排列，伸出爪形伪足贴附于壳聚糖纤维上。

Results The testing results showed that this method with 100% specificity and sensitivity in 300 positive and 100 negative samples of mammalian sera respectively. However, the positive serum samples of Francisella tularensis, yersinia pseudotuberculosis and yersinia enterocolitis could not be deceted by dipstick of ICA.

结果 间接血凝法检测阳性300份血清标本，胶体金法检测全部为阳性，间接血凝法检测阴性100份血清标本，胶体金法检测全部为阴性，两种方法符合率100％；并且鼠疫胶体金法快速诊断盒不与假结核耶尔森氏菌等相关细菌的免疫血清发生交叉反应。

The results were as follows:As shown by in situ hybridization, FPN1 mRNA was located in the cytoplasm and distributed mainly in the cortex, hippocampus, compact part of substantia nigra, caudate putamen and medial habenular nucleus.

在不同脑区FPN1蛋白的分布特点与其mRNA的分布特点基本一致。在大脑皮层，除了分子层以外，其他各层都分布有大量的阳性细胞，锥体细胞的胞体和初级突起呈现明显的强阳性。

The distributions of NOS in the pylorus were similar in both groups.

患儿组贲门部肌层中缺乏 NOS 染色阳性神经纤维，肌间神经节细胞呈 NOS 染色阴性，远端食管及胃底部偶可见散在的 NOS 染色阳性神经节细胞，幽门部 NOS 的分布与对照组基本相同。

The method was successfully used to quantitate the virus load in tiss...

最后用建立的方法对108份临床样品进行检测并同时进行病毒分离，荧光定量PCR方法检测出73份阳性样品且与病毒分离的符合率为100%，而常规RT-PCR只检测出54份阳性样品，表明本荧光定量RT-PCR法在检测猪瘟病料上具有潜在的应用价值。

Methods Sera derived from 226 SLE cases and 183 healthy controls were tested for ANA and all parameters were compared such as sensi- tivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,nega- tive likelihood ratio,result consistency,rank correlation coefficient and kappa of ANA detected by IFA...

方法同时采用IFA法和ELISA法检测血清ANA共409例，其中SLE 226例和健康对照183名，将两种方法检测结果的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比、阴性似然比、结果符合率、判断一致性κ值及秩相关系数进行比较分析；通过ROC曲线比较两种方法的准确度；结果不一致标本进行可提取性核抗原抗体谱检测分析；同时将IFA法检测不同荧光模型样本分别与ELISA结果进行相关分析。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

Results On day 7, 15, 30 after injection with exogenous VEGF, the mice in VEGF group were found more renascent blood vessels, splenic corpuscles, central arteries and peripheral lymphatic sheathes in the autotransplanted splenic tissues than the control group, and eventually formed more integral structure of white pulp, marginal zone and red pulp with much less fibrous tissue, while on day 60 there was no significant difference between VEGF group and the control group.

结果术后7、15、30 d注射VEGF组注射VEGF后较对照组的血管数量明显增多；术后60 d注射VEGF组注射VEGF后与对照组各时相点的组织学特征没有明显差别；术后7、15、30 d注射VEGF组KDR面积密度值高于对照组，术后60 d注射VEGF组 KDR面密度值与对照组比较无显著差别；术后7、15、30 d注射 KDR mRNA表达阳性细胞密度高于对照组，术后60 d注射VEGF组 KDR mRNA表达阳性细胞密度与对照组比较没有明显差异。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。