阳性

The expressions of DNA-PKcs and Id2 in SP cells were strikingly stronger than those in non-SP cells, The localization of DNA-PKcs in SP cells was in whole nucleus but that of the protein in non-SP cells was only in nuclear membrane and nucleoplasm nearby.

SP细胞DNA-PKcs表达的阳性强度明显高于非SP细胞；SP细胞Id2表达的阳性强度明显高于非SP细胞；SP细胞的p16表达的阳性强度明显低于非SP细胞；SP细胞FHIT的表达为阴性或微弱阳性；SP细胞NFκB/p65表达的阳性强度明显低于非SP细胞。

NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.

结果 丰富的NGFR染色阳性神经纤维分布于正常结肠环肌层及粘膜下层，少量分布于纵肌层，NGFR染色阳性神经元分布于肌间神经丛及粘膜下神经丛；NGFR 染色阳性神经纤维在HD无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如，而肌间肥大的神经干及粘膜下神经干为NGFR染色阳性，并且在其外周可见一强阳性染色带。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

The expression of nestin antigen, NSE and GFAP were identified by immunohistochemistry, and the number of NSE-positive cells and GFAP-positive cells were dividedly counted.

结果大鼠胚胎神经管神经上皮干细胞在无血清培养基中可形成大量呈nestin抗原阳性细胞构成的神经球，经传代有血清培养后分化为NSE阳性和GFAP阳性细胞，其中NSE阳性细胞占细胞总数的47.7％，GFAP阳性细胞占细胞总数的39.8％。

ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份，其阳性检出率为96.0%，50份正常鼠血清的阴性符合率达100%，DIGFA分别检测蛲虫阳性鼠血清13份，绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份，前二者均为阴性，后者交叉反应率为5.6%；DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清，两法符合率达96.7%。

However among the experimental groups no positive labeling was found in 2h group, positive cells were labeled in SFO and choroids plexus of lateral ventricles in 6h group, especially in ependymal cells of body part of SFO, but no positive cells were found in the other regions.

观察实验组可见：2小时组未发现阳性结果。6小时组在SFO与侧脑室脉络丛，发现阳性细胞，在SFO体部的室管膜细胞阳性较多；其它部位未见阳性细胞。8小时与16小时组在海马、下丘脑、背侧丘脑、脉络丛等多处均有阳性细胞。

Results The SP-IR and CGRP-IR neurons could not be found in the above-mentioned ganglial plexus.But SP-IR fibers distributed sparsely,CGRP-IR fibers were more densely than SP-IR fibers,especially A-PGP.The population of VIP-IR neurons and fibers were most dense and interweaved ganglial plexus.

结果 在上述三个神经节丛内，未发现SP及CGRP免疫反应阳性神经元，但可见少量SP免疫反应阳性神经纤维分布，CGRP免疫反应阳性神经纤维数量较SP阳性纤维密集，尤其在A-PGP更为明显；VIP免疫反应阳性神经元和神经纤维分布最为密集，交织成神经节丛。

Catenin was detected in suprabasal, epidermal basal layer, placode, hair bud in early phase(E68～93),and expressed strongly in epidermal basal layer, placode, and hair bud, in suprabasal expressed less strongly; in metaphase(E94～184),β-catenin was detected in epidermis, hair peg, and in suprabasal, epidermal basal layer, hair follicle bulge, inner root sheath, outer root sheath, follicular infundibulum expressed less strongly; in late phase(E184～225),β-catenin expressed weakly in epidermal basal layers, while expressed strongly in epidermal keratinocytes.

结果显示：β-catenin在胎牛蹄边缘毛囊形态发生早期(68～93天)表达于表皮、基底层、基板和毛芽中，其中基底层、基板、毛芽中呈强阳性，表皮中呈中阳性；在毛囊形态发生中期(94～184天)表达于基底层、表皮和毛钉中，其中基底层、表皮、隆突区、内外根鞘和漏斗部呈中阳性；在毛囊形态发生晚期(184～225天)只在表皮及基底层有表达，其中基底层呈弱阳性，表皮呈强阳性。

Results: The distribution of NADPH-d products and Fos positive neurons was partly overlapped in the PBN. The NADPH-d positive neurons adjoined the Fos positive neurons in the dorsal lateral subnuclear and some Fos/NADPH-d double labeled neurons were observed. The Fos positive neurons were intermingled with the NADPH-d positive fiber terminals in the external lateral subnuclear.

结果：PBN内Fos阳性神经元与NADPH-d阳性产物的分布范围部分重叠：在臂旁外侧核背亚核内，Fos阳性神经元与NADPH-d阳性神经元相互邻接，并可见部分Fos/NADPH-d双标神经元；在臂旁外侧核外亚核内，Fos阳性神经元与NADPH-d阳性纤维终末相互混杂分布。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。