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Results: Ameloblastin mRNA was negative under microscope in the ameloblast proliferate differentiation period, it showed weakly positive expression in intra-cellular and new-birth enamel matrix in secrete initial stage while it showed strongly positive expression in intra-cellular and new-birth enamel matrix when it came to secretion phase.

结果:釉鞘蛋白mRNA在成釉细胞增殖分化期表达为阴性,分泌初期细胞内及新生釉基质中开始出现弱阳性表达;至分泌期,细胞内及新生釉基质中均呈强阳性表达。

RESULTS: CaB was not detected during early odontogenesis from the dental lamina to the late c ap stage .Small amount of the protein first appeared in ameloblast and some of dif ferenting cells in the surface of dental papilla in early bell stage. As to late bell stage, the cytoplasm of ameloblasts and odontoblasts with secreted dentin was all stained strongly, as well as some of n uclei.

结果:人牙胚蕾、帽状期成釉器无CaB的表达;钟状早期成釉器的内釉细胞及部分牙乳头表面处于分化状态的细胞弱阳性染色;钟状晚期成釉器的成釉细胞、成牙本质细胞及部分胞核呈现强阳性染色。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库,在大肠杆菌中扩增后提取混合质粒DNA,经电转化非缺陷标志酒精酵母 AS.2.1364,在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子,从阳性转化子中分离重组质粒证实含5.0kb的插入片段,用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Results Five DFRs were found. One of the DFR of 383 bp was specific to biovar antiqua. The 383 bp fragment was only found in strains of Y.pestis isolated from Marmota baibacina Spermophilus undulates Plague Focus of the Tianshan Mountains, but not found in strains from other Plague Foci. It was also found in two (belonging to biotypeⅠ and Ⅱ) of five strains of Y.pseudotuberculosis .

结果 发现了5个在不同生物型鼠疫菌间存在差异的DNA片段,其中一个383bp的片段在来自天山山地灰旱獭、长尾黄鼠鼠疫自然疫源地的30株鼠疫菌全部阳性,而来自其它鼠疫自然疫源地的菌株全部阴性,5株假结核耶尔森菌中有2株阳性

In addition, an expression of c-fos gene could be induced in the caudate putamen by injection of apomorphine from the rats survived 2 months in the experiment group.

免疫组化染色结果表明:6-OHDA损毁7 d,损毁侧TH阳性神经元及强阳性神经元较正常侧数目明显减少(P<0.05),但阿朴吗啡不能诱导c-fos表达。

Decreased expression of RIG-I in human hepatocellular carcinomaFirst, with tissue array, we analyzed the expression of RIG-I in HCC, and found that 90%(27/30) of para-tumor samples were positive for RIG-I expression, but only 20%(6/30) of HCC samples were RIG-I positive.

然而,在30例肝癌患者的肝癌组织中,只有5例患者的癌组织中RIG-I蛋白表达为阳性,1例患者的癌组织中RIG-I蛋白表达为弱阳性阳性率为20%。

Final diagnoses at 1-year follow-up were RA in 31 patients, undifferentiated arthritis in 7 (5 self-limiting), and psoriatic arthropathy and antisynthetase syndrome in 1 patient each.

仅7例抗CCP抗体阳性,但最后均诊为类风湿关节炎,在该组血清阴性类风湿患者中的抗CCP阳性率仅23%(7/31),其诊断特异性达100%(95%可信区间为71.7-100),敏感性为23%(95%可信区间为9.6-41.1),而用MRI标准则100%的类风湿患者诊断正确,另外有2例假阳性结果(1例银屑病关节炎和1例抗合成酶综合症),MRI标准对诊断血清阴性类风湿关节炎的特异性为78%(95%可信区间为40.0-97.2),敏感性为100%(95%可信区间为90.8-100)。

Treatment of edaravone markedly reduced the content of MDA and the expression of Caspase-3 in comparison to the ASCI control group, while the expression of Bcl-xl was obviously higher in the ASCI treatment group than that in the ASCI control group.

结果:ASCI盐水组MDA含量、Caspase-3阳性细胞表达和Bcl-xl蛋白表达水平均明显高于假手术组;与盐水组相比,依达拉奉治疗组MDA含量和Caspase-3阳性细胞表达水平均下降,而Bcl-xl蛋白表达水平显著增高。

The results showed that the hereditary mode of rolling tongue or pointed tongue is the dominant heredity of single gene of autosome,and the positive type of them is the dominant character;Twisting tongue is the recessive heredity of single gene of autosome,while the positive type is the recessive character.

采用Penrose先证者同胞法和分离分析方法对72个家系资料的分析表明:卷舌、尖舌均为常染色体单基因显性遗传,阳性为显性性状;翻舌为常染色体单基因隐性遗传,阳性为隐性性状。

Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.

Western blot分析显示:该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应,而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。

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