阳性

There is not a significant difference in these groups by one-way analysis of variance. 2 Among 51 cases with lung cancer, 7 specimens showed malignant cells in bronchial lavages, including 4 cases in central lung cancer and 3 cases in peripheral lung cancer. Concordant results were observed in 20 cases. 6 specimens showed malignant cells on cytological analysis, and NMSP was positive in at least one gene tested. 14 samples did not show malignant cells on cytological analysis, and the NMSP results were correspondingly negative. The results of cytology and NMSP were discordant in 31 samples. 30 samples were cytologically negative, but NMSP positive in one or more genes. In addition, there was one case that the cytology was positive for malignant cells, but the NMSP was negative in all of the three gene tested.

51例肺癌患者支气管灌洗液细胞学检查发现肺癌细胞7例，其中27例中心型肺癌患者4例支气管灌洗液发现肺癌细胞，24例周围型肺癌患者3例支气管灌洗液发现肺癌细胞。20例支气管灌洗液细胞学检查和过甲基化检测得到一致结论。6例支气管灌洗液发现肿瘤细胞，过甲基化分析至少一个基因阳性。14例灌洗液未发现肿瘤细胞，三个基因过甲基化分析均为阴性。31例支气管灌洗液细胞学检查和过甲基化检测得到不一致结论，30例细胞学检查为阴性，但过甲基化分析一个或一个以上基因为阳性。1例细胞学检查阳性但过甲基化分析阴性。

Compared with the control fish, the number of S100-IR cells in poisoned loaches increased significantly and was proportional to exposure time.

与对照组相比，处理组S100免疫反应阳性细胞数显著增多，且该变化与环境中无机汞处理时间成正相关；阳性细胞胞体明显膨胀，突起稠密粗大，S100阳性反应强。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

Immunohistochemical stainings for CK7 and CEA in 6 cases with VP showed strong positive, whereas for 34βE12 and HMB45 in all 6 cases were negative, and for C-erbB2 in 2 cases were positive.

上皮内VP切缘阳性4例，复发2例；切缘阴性4例均无复发。6例CK7、CEA呈强阳性，34βE12、HMB45阴性，2例C-erbB2阳性。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Results （1）The positive rates or the scorings of MMP-1,MMP-9and MVD were significantly higher in human gallbladder neoplasm than that in human chronic cholecystitis（P.01）,But the positive rates of MMP-2in human gallbladder neoplasm were significantly lower than that in human chronic gallbladder cholecystitis（P.01）.（2）The closely positive correlations were found among the expressive scorings ofMMP-1,MMP-9,TIMP-2,MVD and the clinicopathological type,differentiated degree,with or without metastasic tumor.

结果 （1）31例胆囊癌组织中MMP-1，MMP-9的阳性表达率及其评分和MVD计数均值（以CD34阳性计数）分别为16例（51.6%）和20例（64.5%），明显高于10例慢性胆囊炎组织1例（10.0%）和2例（20.0%），差异有非常显著性（P.01）；胆囊癌组织中TIMP-2的阳性表达13例41.9%）则低于慢性胆囊炎组织9例（90.0%），差异有非常显著性（P.01）。

Using electron microscopy, the SPR-LI products in DCN neurons were seen not only in postsynaptic sites, but also in non-synaptic regions of the perikarya and dendritic profiles.

在电镜下证明SPR免疫阳性产物不仅位于突触后部位而且分布于胞体和树突的非突触部位，有42％的SPR阳性树突与SP阳性终末形成突触连结。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4～5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果：模型C组于免疫注射第3周左右开始出现活动减少，倦怠嗜卧，食欲体重下降等表现，模型B组有14只SD大鼠于免疫注射第4～5周出现上述症状，较C组为轻；模型组肌酶谱中，肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高，模型C组较模型B组升高更显著；模型组肌电图时限缩短，波幅降低，多相波增多；磁共振检查模型B组和C组选送标本中各有一例有阳性改变，表现为T1MI等或稍低信号，T2MI及STIR序列为高信号，提示肌肉炎症水肿改变；模型B组有11只，模型C组全部大鼠骨骼肌出现病理改变，表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润，间质小血管壁增厚、扩张，随机选送的心肌标本中有3例有阳性改变，表现与骨骼肌相仿，选送的肺标本中有1例有阳性改变，表现为蛋白渗出，炎症细胞浸润和小血管改变。

Acute pancreatitis patients were grouped as MAP and SAP after 72 h according to the clinical manifestations. Results:All the indexes were significantly higher in the AP group（P.05）. Urinary trypsinogen activation peptide and serum procalcitonin were increased significantly after 48 h.The sensitivity、specificity and positive likelihood rate of TAP and PCT were 90.5％,87.5％,7.2 and 90.5％,82.5％,7.2 respectively.

结果：急性胰腺炎组与非急性胰腺炎组各项检测结果均存在显著性差异（P.05），各检测指标的敏感度、特异性和阳性似然比存在一定差异，尿TAP和血清PCT在入院48 h后统计效能最高，尿TAP敏感度、特异度以及阳性似然比分别为90.5％、87.5％和7.2；血清PCT敏感度、特异度及阳性似然比分别为90.5％、82.5％和7.2。

The results showed that the POD existed in the tentacles, respiratory tree and digestive system of Apostichopus japonicus. The activity of POD in epidermic layer of body wall and the outer layer of alimentary canal were weakly positive. POD in epidermis of tentacles were positive.

研究表明，刺参触手、体壁、呼吸树和消化系统中均有POD的存在，但体壁上皮层和消化道外膜中的POD呈弱阳性；触手表皮层内的POD为阳性；呼吸树的粘膜层和粘膜下层，呈强阳性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。