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The result was consistent with that of double-disk synergy tests. 61 strains were found to be ESBLs negative detected using VITEK GNS-506. Among them, 19 strains were found to be ESBLs positive.

检测102株大肠埃希菌中,仪器检出41株阳性,纸片协同法对照也为阳性,但仪器检测的61株阴性菌中,纸片协同法对照有19株为阳性

The average content of aflatoxin Blin silage,coarse fodder and blend diet is 8.28μg/kg, 3.11μg/kg,and 6.56μg/kg.respectively,the pollution is light.50 samples have been detected are all accorded with the limited standard in our country,the maximum of positive is 0.203μg/kg,the minimum is 0.003μg/kg,and the average value is 0.089μg/kg.

被检测的青贮料、粗饲料、全混合日粮样品中黄曲霉毒素B1平均含量分别为8.28μg/kg、3.11μg/kg和6.56μg/kg,属于轻度污染。50份奶样中,阳性最高值为0.203μg/kg,阳性最低值(来源:ABC论文f1网www.abclunwen.com)为0.003μg/kg,阳性平均值为0.089μg/kg,均符合我国目前的限量标准。

Methods According to the levels of thyroid auto-antibodies,622 patients with hyperthyroidism were divided into four groups:group A 54 cases with negative TMAb,TGAb and TRAb,group B 176 cases with nagative TMAb and TGAb and positive TRAb,group C 78 caese with positive TMAb,TGAb and ne...

方法根据甲状腺自身抗体水平对622例拟行碘-131治疗的甲亢患者进行分组。A组TMAb、TGAb、TRAb均阴性,54例;B组TMAb、TGAb阴性,TRAb阳性,176例;C组TMAb、TGAb阳性,TRAb阴性,78例;D组TMAb、TGAb、TRAb均阳性,314例。

Results During the committed differentiation, the expression of calmodulin was distributed in and around the cell nucleus.

结果在神经干细胞定向分化为神经元的过程中,钙调蛋白于细胞核及核周呈阳性表达,随分化神经元的生长细胞核阳性表达逐渐减弱而胞浆增强,同时可见阳性反应物伸入树突及轴突。

A total of 990 samples were collected from chickens' ovaries and duodenums. The Salmonella - positive rate of these chickens was 25.5%(252/990) and of all specimens was18.2%(359/1970). The percentage of Salmonella-positive specimens of ovaries was 1.4 times more than duodenums 95 % C.

采样自蛋鸡卵巢与十二指肠部份,总计采取990只蛋鸡,鸡只的阳性率为25.5 %(252/990);沙门氏菌的样本总阳性检出率为18.2 %(359/1970),卵巢的沙门氏菌阳性检出率为蛋鸡十二指肠沙门氏菌阳性检出率的1.4倍 95 % C。

Results The body weight, visceral fat and body weight ration, as well as the levels of blood glucose, insulin, triglyceride, total cholesterol, LDL, and total FFA, were significantly lower in high fat-fed group than in control group (P.01). The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups.

结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。

The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups. Little or no expression of NF-κB, ICAM-1 and MCP-1, and a high expression level of IκBα were detected in aortic tunica intima of the n-3 PUFA and control groups, while high expression levels of NF-κB, ICAM-1 and MCP-1, and little expression of IκBα were detected in aortic tunica intima of the high fat-fed group.

结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。

Results:CKH,CK8 and CK19 were positive in spindle-shaped and polygonal epithlium tumor cells.Vimentin was positive in mesenchymal fibrocytes and smooth muscle actin in some mesenchymal cells.

结果:免疫组化标记表明,肿瘤上皮中梭形、多边形细胞CKH、CK8、CK19呈不同程度阳性,间质中纤维细胞vimentin阳性,部分间质细胞smooth muscle actin阳性

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时,本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上,经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后,得到阳性重组质粒pET32a-σC;将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达,经SDS-PAGE和Western-blbtting检测分析,融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应;将融合表达的蛋白纯化后作为包被抗原,建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法,此方法中抗原的最佳包被浓度为5μg/ml、标准阳性血清的最适稀释倍数为1:40倍,用此方法对50份鸭血清样品进行检测,并与琼脂糖凝胶扩散试验检测抗体的法相比较,证明此ELISA方法具有良好的特异性和敏感性,本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增,均出现371 bp长度的DNA片段,敏感性试验可检测出10-12 g的细菌DNA,与人类基因组DNA、真菌及病毒无交叉反应;22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带,反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

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