阳性

In the visual system, 5-HT/TPH-like immunoreactiveneurons (ca.35 per hemisphere) were mainly situated between the optic lobe and lateralprotocerebrum, the neurites of which either descended into the optic neuropils or ascendedinto the protocerebrum, thus possibly integrating the optic lobes and other brain regions. Inthe protocerebrum, 5-HT/TPH-like immunoreactive neurons (ca. 25) mainly distributed dorsalto the protocerebrum beside the Kenyon cells. Their axons directly projected into theprotocerebrum as well as the optic lobe.

TPH阳性神经元的类型、数目、分布位置、投射区域与5-HT基本相同。5-HT/TPH阳性神经元在视觉系统主要分布于视叶与侧前脑之间，每侧大约有35个，其轴突及分枝或下行进入视叶或上行进入前脑。5-HT/TPH阳性神经元在前脑分布于前脑背方、肯氏细胞两侧，大约有25个，轴突主要投射到蕈形体和中央复合体的广泛区域，另有个别神经元轴突下行投射进入视叶形成广泛分枝。

RESULTS: HIF-1α positively expressed at stratum neuroepitheliale retinae and purpurogenous membrane in the embryonic phase. Additionally, HIF-1α still positively expressed at stratum neuroepitheliale retinae and purpurogenous membrane, especially in ganglionic cells and inner plexiform layer, in early development.

结果：胚胎期视网膜神经上皮层及色素上皮层均有低氧诱导因子1α阳性表达，生后发育早期视网膜神经上皮层及色素上皮层也可见低氧诱导因子1α阳性表达，以神经节细胞、内网层更明显，随着发育进展，其阳性表达主要位于视网膜节细胞层。

Immunohistochemical assay were performed with HGV NS5 McAb. Results One of four samples showed positive staining, some of which located in cytoplasm and some of which in cytonucleus and cytomembrane. The number of positive staining cells are not more. More of them disrtibuted scatteredly, some of them located.

结果 1例呈阳性染色，光镜观察显示：特异染色大部分定位于肝细胞胞浆中，部分有核着色和膜型染色，呈黄色或棕黄色颗粒状，阳性细胞数量少，大多散在分布，部分呈灶性分布；阳性细胞周围可见较多淋巴细胞浸润。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/μl.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

Results: nk 4r immunoreactivity was found extensively localized in neuronal somata and neurite of cerebral cortex, and mainy in layer ⅲ,ⅴ. double labeling experiments confirmed that 100% nk 4r immunoreactive neurons co expressed neun, but not gfap.

结果：nk 4r免疫阳性产物在皮层的锥体细胞层分布最多，在皮层内、外颗粒层、多形层也广泛分布，其阳性产物表达于神经元胞体和突起上，免疫荧光双标结果观察到nk 4r与神经元胞核特异性标记物neun共存（100%，146/146，双标细胞数目/nk 4r阳性细胞数目），且不与星形胶质细胞标记物gfap共存。

It was seen that the main microflora causing the spoilage of fresh mutton meat consisted of 3 gram-positive bacilli with spore (about 30% of the micro flora), 1 gram-positive coccus (about 2% of the whole microflora), 3 gram-negative spirilla (about 63% of the whole microflora), and 1 gram-negative bacillus (about 5% of the whole microflora). Potassium sorbate, EDTA and nisin were used as preservatives to inhibit the growth of the main strains representative for the spoilage of fresh lamb meat.

对屠宰后羊肉的微生物区系进行了调查，成功地分离出引起冷却羊肉腐败的主要革兰氏阳性菌和阴性菌8种，其中含芽孢的革兰氏阳性杆菌有3种，占整个微生物区系的30％；革兰氏阳性球菌1种，占整个区系的2％；革兰氏阴性螺旋杆菌3种，占整个区系的63％；革兰氏阴性杆菌1种，占整个区系的5％。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P＞0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P＜0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P＜0.05).The levels of CGRP and SP in CSD group were higher than control group(P＜0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P＞0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P＜0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P＜0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P＜0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P＜0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD；同侧TNC放电频率，CSD中＞CSD后＞CSD前P＜0.05对侧TNC放电频率，CSD前、中、后无统计学差异(P＞0.05氟桂利嗪组同侧TNC放电频率，CSD前＞CSD中(P＜0.05)，CSD前与CSD后及CSD中与CSD后之间无统计学差异(P＞0.05)。2.1关于放免测定，各组血浆CGRP、SP水平有统计学差异(P＜0.05)，CSD组高于对照组(P＜0.05)，CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P＞0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P＞0.05.2关于免疫组化研究，右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P＜0.05)，多重两两比较结果CSD组大于对照组(P＜0.05)，CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P＞0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P＞0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P＜0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P＜0.05，蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P＜0.05，false discovery rate，FDR校正疼痛状态与非疼痛状态比较，同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P＜0.05，蒙特卡罗模拟校正。

Hypoxia-ischemia for 10 minutes group , 20 minutes group: our experiment showed that at 24 hours after cerebral hypoxia-ischemia, expression of MMP-9mRNA was maximally increased, individually, in the hypoxia-ischemia for 10 minutes group, MMP-9mRNA was greatly expressed within superolateral surface cortex of cerebrum, whereas in the hypoxia-ischemia for 20 minutes group, the labeling of hybridization for MMP-9mRNA was strongly appeared within the cortexof cerebrum both the cortex in the subsequence of medial surface at the periphery of the cerebral longigudinal fissure and the cortex in superolateral surface where also there showed a few stained fibers.

3缺氧缺血10分钟、20分钟组：缺氧缺血后24小时脑内MMP9 mRNA阳性表达至最高峰，缺氧缺血10分钟组大量表达在端脑背外侧面皮质内，缺氧缺血20分钟组在端脑内侧面后份大脑纵裂旁周围皮质及背外侧面皮质内呈强阳性表达，少数纤维呈亦阳性染色。

Results:① Neuroglobin-immunocreactive positive cells were detected in cortex, thalamencephalon, hippocampus, hypothalamus, cerebellum, brain stem and spinal cord;② Ngb-IR positive nerve cells were also detected in peripheral nervous system such as intramural ganglia, myelinated nerve fiber and retina;?Substantial Ngb protein expression was also found in metabolically active endocrine tissues such as the pituitary gland , adrenal gland and testis.?

结果：(1)在所观察的大脑皮质、海马、丘脑、下丘脑、脑干、小脑和脊髓神经细胞都出现Ngb蛋白免疫阳性反应，不同区域表达强度不同：(2)在外周神经系统的神经节、有髓神经纤维和视网膜也观察到Ngb免疫阳性反应；(3)垂体、肾上腺、睾丸也可见到Ngb-IR阳性细胞。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。