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We purified the target protein under natural conditions by MCAC through Ni-NTA resin. The purity of this fusion PH domain of IRS-1 was up to 98%. We paned out the oligopeptides that can bind to this target protein from a phage displayed random duodecimal peptide library. The identities of peptide sequences of positive clones were analyzed.

因此,我们利用融合表达载体pRSET在T7启动子的调控下表达出氨基端带六个连续组氨酸标签的IRS-1的PH结构域,利用Ni-NTA介质在非变性条件下对目的蛋白进行了金属螯合亲和层析纯化,获得了纯度达98%以上的PH结构域融和蛋白,以此为靶分子,从噬菌体展示随机12肽库中筛选出能够与其相结合的短肽,并分析了阳性克隆的序列一致性。

Result:There were 166 cases that were abnormal in 175 cases ,the incidence of peptic ulcer was 15.43%, the incidence of duodenal ulcer was higher than gastric ulcer in children.the incidence of chronic gastritis and chronic duodenitis was 94.86%, the incidence of chronic gastritis was significantly higher than chronic duodenitis, chronic superficial gastritis, bile reflux gastritis see most, chronic erosion hemorrhage of chronic gastritis and chronic duodenitis was not uncommon.

结果:175例胃镜检查有异常者为166例,消化性溃疡发生率为15.43%,小儿十二指肠溃疡发生率高于胃溃疡,并以十二指肠球部溃疡多见,胃溃疡以胃窦溃疡多见,胃、十二指肠炎的发生率为94.86%,小儿慢性胃炎发生率明显高于慢性十二指肠炎,其中以慢性浅表性胃炎、胆汁返流性胃炎最多见,慢性糜烂出血性胃、十二指肠炎也不少见,幽门螺杆菌检测阳性率为59.04%,其中消化性溃疡病例检测幽门螺杆菌均为阳性

Result:There were 166 cases that were abnormal in 175 cases ,the incidence of peptic ulcer was 15.43%, the incidence of duodenal ulcer was higher than gastric ulcer in children.the incidence of chronic gastritis and chronic duodenitis was 94.86%, the incidence of chronic gastritis was significantly higher than chronic duodenitis, chronic superficial gastritis, bile reflux gastritis see most, chronic erosion hemorrhage of chronic gastritis and chronic duodenitis was not uncommon. Helicobacter pylori testing positive rate of 59.04%, peptic ulcer cases were all positive detection of helicobacter pylori.

结果:175例胃镜检查有异常者为166例,消化性溃疡发生率为15.43%,小儿十二指肠溃疡发生率高于胃溃疡,并以十二指肠球部溃疡多见,胃溃疡以胃窦溃疡多见,胃、十二指肠炎的发生率为94.86%,小儿慢性胃炎发生率明显高于慢性十二指肠炎,其中以慢性浅表性胃炎、胆汁返流性胃炎最多见,慢性糜烂出血性胃、十二指肠炎也不少见,幽门螺杆菌检测阳性率为59.04%,其中消化性溃疡病例检测幽门螺杆菌均为阳性

However, this parameter has been reported to be falsely positive, especially in patients with right ventricular dysfunction.

然而,这一参数有假阳性结果,尤其在右心室功能障碍的患者。

The total scores, positive items and factors scores of depression, anxiety, obsessive-compulsive and somatization in FD group were significantly higher than control group, the correlations between dyspeptic symptoms and factors scores of anxiety and depression were slightly closer than other factors.

FD患者SCL-90总分、阳性项目数及各因子分高于正常对照组,差异有统计学意义,抑郁和焦虑因子与胃肠症状关系较其他因子密切; 3。

Our study confirmed that the frequency of ubiquitin positive neuronal intranuclear inclusions and dystrophic neurites were varied markedly in different area of cerebral cortex. Owing to the marked neuropathological changes in frontal cortex,the cognitive symptoms should be considered as a frontal -subcortical dementia.

大脑皮层出现泛素阳性神经细胞核内包涵体和营养不良神经突起在大脑不同区域的分布存在很大的差异,由于额极也存在明显的病理改变,所以此病的智能减退属于额叶皮层-皮层下性痴呆。

ConclusionMany factors influenced the false positive in auditory screening of neonate, a set of nursing intervention including cleaning the ear wax, select the suitable opportunity of test and the suitable ear plug, can cut down the false positive rate.

结论多种因素可导致新生儿听力筛查假阳性,采取清除耳道分泌物、选择合式的测听时机、选择合适耳塞等护理对策,可使新生儿听力筛查的假阳性率显著降低。

Five (22%) of the 24 T-cell tumors expressed βF1, 8 (35%) of the 23 tumors were positive for EBER, and 20 (95%) of the 21 tumors were clonal for T-cell receptor.

那24例T细胞淋巴瘤中有5例(22%)表达βF1;有23例做了EBER检测,其中8例(35%)阳性;有21例做了T细胞受体基因检测,其中20例(95%)为多克隆性重排。

The molecular weights of the 11 major bands were 82, 79, 60, 51, 46, 38, 32, 30, 28, 24 and 18kD, respectively. The results of Western blotting with the positive serum of anaphylactic patients showed that the 1st-4th instar larvae all had the specific allergens whose molecular weights were 82 and 79kD, respectively; only the 5th instar larvae all had the specific allergen of 30kD. We purified the protein of 30kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.

选用家蚕过敏患者阳性血清进行免疫印迹,1~4龄家蚕均显示出82和79kD的特异性变应原;但只有5龄家蚕的30kD蛋白为特异性变应原,通过离子交换层析和经切胶纯化出30kD蛋白,再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。

Mu opioid receptor was not observed in the wrist, funnel, abdomen of mantle, mouth, craw, stomach theca, intestine, rectum, sialoid gland, liver. Weak positive mu opioid receptor immunoreaction was found in inner epidermis, ectoblast, connective tissue of the esophagus and the keratolytic of the stomach and positive immunoreaction in epidermis of back of mantle and membrane of wrist.

结果表明,短蛸腕、漏斗、外套膜腹面、口球、嗉囊、胃盲囊、肠、直肠、前唾液腺、后唾液腺、肝胰脏均呈μ受体阴性,但食道内上皮、外膜、结缔组织和胃角质层有μ受体分布,外套膜背面、腕间膜的上皮或近上皮部位呈μ受体阳性

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