间隔区

The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma , then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers.

以特异性引物扩增了金焰笛鲷的核糖体第一转录间隔区（ITS-1），扩增产物经克隆后测序，测得ITS1长度为566 bp。

This paper examines the sequence and organization of the ribosomal RNA gene,the ITS region of Nosema antheraeae,and present molecular biological evidence of Nosema antheraeae taxonomy.

目的研究柞蚕微孢子的核糖体基因，转录间隔区以及它们的排列顺序，为柞蚕微孢子的分类和系统进化分析提供分子生物学方面的依据。方法采用特异引物进行PCR扩增，克隆和测序。

Nosema antheraeae ; Ribosomal RNA gene ; ITS ; Organization ; Phylogenetic analysis

柞蚕微孢子；核糖体基因；转录间隔区；排列顺序；系统分析

Based on the analyses of the obtained sequences and the sequences from other three representative species of Oscillatoriaceae, and two species of Microcystis , the evolutionary affiliation among the genera of Oscillatoriaceae was discussed.

并通过计算机联网从国际分子生物学数据弹库中获取颤藻科其它种的rDNA基因间隔区序列，通过比较分析，从分子水平对颤藻科Oscilatoriaceae属间的某些分类学问题进行了讨论，并根据序列中核苷酸差异值探讨了颤藻科属间界定的分子标准。

Oscillatoriaceae ; rDNA Intergenic spacer region ; Sequence analyses ; Taxonomic significance

颤藻科； rDNA间隔区；序列分析；分类学意义

Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 )，并直接用于测序从而获得该基因的核苷酸序列。

Objective: To identify and distinguish common staphylococcal species by developing a real-time PCR assay with melt curve analysis.

目的： 建立一种基于葡萄球菌16s～23s rRNA间隔区序列特征的荧光定量PCR/熔解曲线分析鉴定。

ITS2(Intemal Transcribed Spacer 2) region in ribosome DNA has a character of conservative that makes a low aberrance ratio, on the other side, ITS-2 region evolves itself. According to the characters, ITS-2 region sequencing is an effective parameter in taxology and pests-control..

核糖体基因的第二内转录间隔区有较高的保守特性，同时进化速度又较快，因此序列进行测序得出的种内差异，是系统发育学的有力参数，也有助于异色瓢虫分类学和农林虫害的防治。

To study the taxonomy position of two Trypanosoma evansi strains from buffalos in Guangxi and Hubei provinces, the ITS (ITS1-5.8S-ITS2) gene of the T. evansi were amplified by PCR and sequenced.

为研究从水牛分离到的伊氏锥虫广西株和湖北株的分类学地位，对2株伊氏锥虫的核糖体基因内转录间隔区(ITS1-5.8S-ITS2)基因进行了克隆和测序分析。

In acute injuries, bone contusions are manifested as regions of edema in the subchondral marrow, typically in the lateral compartment.

在急性损伤时，骨挫伤表现为软骨下骨髓内多数区域内水肿，典型者多位于膝外侧间隔区。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。