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Objective: To study the influence of early diagnosis and treatment of osteofascia compartment syndrome on limb.

目的:探讨骨筋膜间隔区综合征的早期诊断及治疗对肢体的影响。

Based on macromorphological and micromorphological characters, as well as conidium ornamentation observed with scanning electron microscopy, Aspergillus sp. D-l was affiliated to Aspergillus Section Versicolores. Further, DNA sequence of the internal transcribed spacer was used as the molecular characteristic, and analyzed with the DNAMAN 5.2.2 program and the Mega 3.0 program.

采用真菌核糖体基因内转录间隔区序列作为分子标记,利用DNAMAN 5.2.2和Mega 3.0等软件对其进行同源性和进化关系分析,在种的水平上将Aspergillus sp.D-1归属为Aspergillus versicolor,并将该菌株命名为A.versicolor D-1。

Cloning and sequencing of the genes coding a and 3 subunit of phycoerythrin of a red alga-Corallina officinalis are reported firstly(GenBankaccession number: AF510986). The sequence contain 1,140 nucleotide, including 10 nucleotide that locate in the upstream of the initiation codon ofP subunit, 534 coding for P subunit(coding 177 amino acid), 101 nucleotide for gene spacer and 495 nucleotide that draw near the 5 terminal of a subunit(coding 164 amino acid).

首次测定了珊瑚藻藻红蛋白α和β亚基基因的全序列(GenBank登录号:AF510986),测定的基因序列长为1,140个碱基,包括β亚基起始密码子上游的10个碱基,6亚基编码区的534个碱基(编码177个氨基酸),101个碱基的间隔区以及α亚基编码区近5'端的495个碱基(编码164个氨基酸)。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序,将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较,结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3%,与其它毒株的同源性在58.3%~91.7%之间;狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

The internal transcribed spacer regions of nuclear ribosomal DNA from 8 species of subgenus Amygdalus were sequenced, and analyzed together with other ITS data of 18 species representing subgenus Cerasus, subgenus Armeniaca and subgenus Primus by using of Padus racemosa Gilib. and Padus buergeriana Yüet Ku as outgroup for studying phylogeny of subgenera in stone fruit plants.

采用核核糖体DNA内转录间隔区对桃亚属的光核桃、甘肃桃、新疆桃、山桃、陕甘山桃、扁桃、野扁桃基因序列测序及来源于GenBank的李、杏、梅、樱的18个种的ITS区基因序列,以稠李和楼木、梅和杏分别作为对桃亚属及其近缘亚属植物、桃亚属各种类的外类群,进行系统发育树的建立。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后,利用体外结合的方法发现,蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力,尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段,也能明显地结合于酵母核骨架,这一结果表明二者的SAR顺序具有一定的同源性,为骨架结合区域具有进化保守性提供了又一证据。

The 1 kb scaffold-attachment region at 5' non-transcription region of rRNA gene of silkworm Attacus ricini was cloned into eukaryotic expression vector pLu, which contained luciferase report gene and neo selecting marker.

蓖麻蚕核糖体RNA基因非转录间隔区有一长约1kb的核骨架结合区(scaffold-associated region,SAR)。

The sequence of rDNA Intergenic Spacer Regionwas determined for the species Oscillatoria sp.. The spacer sequences was 427bp in lengh exclusive of 16S and 23S rDNA coding regions,containing a Ile tRNA gene.

采用末端终止法对蓝藻类颤藻科Oscilatoriasp.rDNA16S-23S基因间隔区进行了序列测定,获得了Oscilatoriasp.rDNA基因间隔区427个核苷酸,其中包含1个异亮氨酸tRNA基因。

In the spacer region, elements related to transcriptive initiation and termination were identified

基因间隔区存在一定的保守单元,其中一些与转录的起始和终止调控相关,有些是裸子植物未知功能的特异保守区。

Put in spacers (you can make the spacers with PVC pipe) to line up the cog size ...

提出在间隔区(你可以作出间隔与PVC管)线了焦炉煤气的大小。。。

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