闭壳

In order to search for a suitable method for the tissue preservation and DNA isolation of marine animals, the adductor muscle of bay scallop was treated with different methods: frozen at -20 ℃, fixed with 70% ethanol and 10% formaldehyde more than 10 days.

以海湾扇贝为材料，研究了鲜活样品以及采用冷冻保存、70 %乙醇固定和10 %福尔马林固定等方法保存的闭壳肌样品用于DNA提取的不同效果，并分析了不同保存条件下所提得DNA用于RAPD扩增的结果。

Protein and triglyceride content in adductor muscle increased during gametogenesis, and then deceased in spawning.

在非繁殖期二倍体和三倍体性腺和闭壳肌生化成分含量受倍性影响不大。

PART B As you have seen during the dissection, the three bivalves show differences in their muscles.

在解剖过程中可以观察到，这三种双壳贝的闭壳肌存在著差异

The lowest energy wavefunction is a singlet biradical instead of a closed shell singlet.

1最低能量波函数是单重态的双自由基而不是闭壳层的单重态。

Money turtle scientific name three Cuora trifasciata, commonly known as the Tri-closed-shell turtles, red belly turtle, golden-headed turtle, etc. belong in the animal taxonomy Reptilia, Testudines, turtles Division .

金钱龟学名为三线闭壳龟，俗称三棱闭壳龟、红肚龟、金头龟等，在动物分类学上隶属于爬行纲、龟鳖目、龟科。

Nevertheless, the results strongly supported that 1 Pyxidea mouhotii and Cuora aurocapitata formed a monophyletic clade, whereas Cyclemys atripons was not closer to the Pyxidea-Cuora than to Chinemys reevesii, suggesting that Cyclemys and the Cuora group may have originated from two ancestors; 2 the Geoemydidae with Testudinidae was a sister group rather than with the Emydidae.

结果表明：锯缘摄龟和金头闭壳龟构成单系支，白腹摄龟与此聚类支的亲缘关系较乌龟远，暗示齿缘摄龟属和闭壳龟组可能起源于两个不同的祖先；淡水龟科和陆龟科而不是龟科互为姐妹群。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析，结果表明，甲基化程度存在差异的位点46个，其中18个位点为甲基化或半甲基化位点，推测橘红色闭壳肌的发生可能与这些位点有关，它们调控了该性状的表达。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量，结果表明，虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin，白色闭壳肌中未检测到类胡萝卜素，其中肝胰腺除上述两种类胡萝卜素外，还含有其他多种未鉴定的类胡萝卜素；在各组织中，雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高；橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝；雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察，并未观察到异常现象；超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌，在橘红色闭壳肌中有三种肌纤维，其中疏松型肌纤维为橘红色闭壳肌特有。

This is the first report of pectenolone and pectenoxanthin, two types of carotenoid, in the muscle of the Yesso scallop.3 Roles of the carotenoids composition and accumulation in Yesso scallopThe method for detecting carotenoids using reverse phase high performance liquid chromatography was developed. The conditions of RP-HPLC analysis were an analytical scale (200×4.6 mm, inner diameter 5μm) Eclipse XDB-C18 column of Agilent corporation; 10μl injection volume; 25°C column temperature; acetonitrile, methanol and methylene dichloride mobile phase (40:56:4, V/VV) with 0.1% BHT; 1.0 ml per minute flow rate; 450 nm UV-VIS detector; 8 minutes elution.The carotenoids from orange muscle of Yesso scallop of 7, 10, 12, 15, 18, 22 months old was detected using the method refered above.

通过柱层析、高效液相等手段对橘红色闭壳肌中的主要类胡萝卜素进行了提取分离，分离提纯得到两种类胡萝卜素，并通过MS、~1H-NMR和~(13)C-NMR对其进行结构鉴定，结果表明(3S，3\'R)-3，3\'-dihydroxy-7\'，8\'-didehydro-β，β-Caroten-4-one和(3S，3\'S)-7，7\'，8，8\'-tetradehydro-β，β-Carotene-3，3\'-diol，俗称pectenolone和pectenoxanthin是橘红色闭壳肌中的主要类胡萝卜素，这是国内外首次对虾夷扇贝闭壳肌中的类胡萝卜素进行提纯及结构鉴定。3虾夷扇贝类胡萝卜素的种类组成和累积变化规律建立了橘红色闭壳肌中类胡萝卜素的HPLC分析定量检测技术：色普柱为Agilent公司的Eclipse XDB-C18柱200mm×4.6mm，i.d.5μm进样体积，5μl ；流速1ml/min；检测波长450nm；柱温25℃；流动相为乙腈：甲醇：二氯甲烷(V/V/V=40：56：4并加入0.1%的BHT；分离时间为8min。

The results indicated that four SOD bands were identified in five tissues, and the band with the biggest mobility ratio were found weakly in gill, digestive gland and adductor muscle but not in foot and mantle. Two ATPase bands were found in four tissues except digestive gland. One MDH band was found in five tissues, but the MDH gene expressed strongly in foot and adductor muscle. Two ME bands were examined in foot, adductor muscle and mantle, but the band with smaller mobility ratio was found in gill and digestive gland.

结果表明，SOD在紫贻贝的5种组织中均有表达，共有4条酶带，但迁移率最大的酶带在足和外套膜中未出现，而在另外3种组织中呈弱表达；ATPase在除消化腺外的组织中都表现为2条带；MDH在5种组织中都表达出1条酶带，其中足和闭壳肌的MDH染色最深；ME存在1~2条酶带，其中迁移率大的条带在5种组织中都有表达，而迁移率小的条带只存在于外套膜、闭壳肌和足中。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了