长肌

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

The results are as follows.(1) Long train electrical stimulation at the dMNF facilitated myoelectric activities of genioglossus and phrenic muscle.(2) Short train electrical stimulation of dMNF, when delivered in the inspiratory phase, caused prolongation of myoelectric activities of genioglossus and phrenic muscle; but when delivered in the expiratory phase, myoelectric activities of genioglossus and phrenic muscle were shortened.(3) Long train electrical stimulation at the vMNF inhibited myoelectric activities of genioglossus and phrenic muscle.(4) Short train electrical stimulation of vMNF caused shortening of myoelectric activities of genioglossus and phrenic muscle, when delivered in the inspiratory phase, but a prolongation when delivered in the expiratory phase.(5) The effects of microinjection of glutamate to dMNF and vMNF were similar to those of electrical stimulation.

结果如下：(1)长串电脉冲刺激dMNF引起颏舌肌和膈肌肌电活动明显增强；(2)短串电脉冲刺激dMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动延长，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动提前终止；(3)长串电脉冲刺激vMNF使颏舌肌和膈肌肌电活动明显被抑制；(4)短串电脉冲刺激vMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动提前终止，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动延长；(5)在dMNF和vMNF分别微量注射谷氨酸钠，其效应与电刺激结果基本一致。

The results showed that the peak latency and pattern of SEPs elicited by electrical needling in LI-l1 and MP were similar. The amplitude of SEPs elicited by electrical needling in LI-l1 was higher than that of MP. There was no obvious SEPs generation when MM was electrical needling.

结果显示，电针刺激曲池穴和其邻近桡侧伸腕长肌运动点诱发性脑电波的波形和波峰时间是类似的，只是穴位的诱发性脑电波波幅较大，而电针刺激非穴位点并没有明显的诱发性脑电位。

The main fatty acid from longissimus, kidney and tailhead fat obtained from each carcass were measured by gas chromatography technology.

日粮中分别添加相当于40.0g粗脂肪的油菜籽、油葵籽、红花籽和亚麻籽，饲喂成年母羊35d后屠宰，用气相色谱技术测定了羊体背最长肌、肾脏脂肪和尾部脂肪的主要脂肪酸组成，结果表明：日粮对肌肉中FA组成的影响较对脂肪组织的大；在所测定的3个部位中，C14：0最为稳定，不受日粮影响；在脂肪组织中，C16：1、C18：1也不受日粮的影响，但肌肉中C18：1含量因添加红花籽显著增大；添加亚麻籽有提高脂肪组织中C18：3的趋势，其余油籽有使各部位C18：3含量下降的趋势，以油葵组下降最为明显；除油菜籽（富含C18：1）外，富含多不饱和脂肪酸的油籽有使C16：0降低的作用。

Beef carcass ; quality determination ; longissimus dorsi ; Ohta color system ; image processing ; mathematical morphological operation

牛胴体；质量评判；背长肌； Ohta颜色空间；图像处理；数学形态学运算

Among them,"8" shaped suture was used in 8 cases, aponeurosis flap repair in 30 cases, transfer repair of tendon of peroneus longus muscle in 2 cases, reverse "V Y" shaped tendon plastic operation in 10 cases, and mattress suture of opposite ends in 12 cases.

对1961 年1994 年80 例采用手术治疗的跟腱断裂中的62 例临床资料进行分析，其中采用&8&字缝合法8例，两条腱膜瓣下翻修复30 例，腓骨长肌移位修复2例，倒&VY&形腱成形术10 例，对端褥式缝合12 例。

Anatomical relationships. B, That part of peroneus longus that arises from lateral surface of fibular head proximal to common peroneal nerve has been detached, allowing nerve to be retracted over fibular head.

解剖关系； B 起于邻近腓总神经的腓骨头外侧面的腓骨长肌已被分离，使得腓总神经被牵到腓骨头上方。

In the 20 years of a career in medicine career, he was physically disabled orthopedic, pediatric cerebral palsy clinic in its way to innovation carried out, such as minimally invasive Achilles tendon lengthening and improving triple arthrodesis, improving the peroneus longus transfer operation and a series of patients type, opened up a new era of micro-orthopedic surgery, with minimal trauma, bringing the maximum recovery of limb function to relieve the patient's pain.

在20多年从医生涯中，在肢残矫形、小儿脑瘫诊疗方面独辟蹊径，创新的开展了如微创跟腱延长术、改良三关节融合术、改良腓骨长肌内移术等一系列术式，开辟了矫形外科的微创新时代，以最小的创伤，带来最大限度的肢体功能的恢复，解除病人的病痛。

Anatomical relationships. B, That part of peroneus longus that arises from lateral surface of fibular head proximal to common peroneal nere has been detached, allowing nere to be retracted oer fibular head.

解剖关系； B 起于邻近腓总神经的腓骨头外侧面的腓骨长肌已被分离，使得腓总神经被牵到腓骨头上方。

Expose the nerve first at the posteromedial aspect of the distal end of the biceps femoris tendon and trace it distally to where it winds around the neck of the fibula. In this location the nerve is covered by the origin of the peroneus longus muscle. With the back of the knife blade toward the nerve, divide the thin slip of peroneus longus muscle bridging it. Then displace the nerve from its normal bed into an anterior position.

先在股二头肌腱远端后内方暴露腓总神经，顺神经向远端追踪至环绕腓骨头的部分，此处腓总神经被腓骨长肌起点覆盖，保持刀背指向腓总神经，挑断跨越神经的部分腓骨肌纤维，然后将腓总神经自正常的位置转移至前移的位置。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了