长春新碱

Objective:To study the cytotoxic activity of DNR and VCR on HL-60 cell line in vitro.

目的 ：观察柔红霉素、长春新碱联合应用在体外对人急性髓系白血病细胞株(HL—60)的相互作用。

Focal therapies were applied to Group A, systemic chemotherapy (CCTV protocol, Cyclosporine, Carboplatin, Teneposide and Vincrinstine) plus ocular surgery (include focal therapy, enucleation and orbital exenteration) to Group B, C, D and E. Patients were followedup for more than 6 months. Result Of 66 cases (36 male/30 female), 16/66 cases were bilateral RB and 50/66 were unilateral.

按照国际眼内视网膜母细胞瘤分期系统分期，A期选择局部治疗（包括激光光凝和冷冻疗法），B、C、D、E期在系统化疗（CCTV方案，环孢霉素、卡铂、替尼泊苷、长春新碱）的基础上，联合手术（包括局部治疗、眼球摘除和眶内容剜出）治疗。

This is a retrospective case analysis study; Review 66 RB cases in Beijing Tongren Hospital from August 2006 to September 2007, diagnosed by ultrasound, radiology examination (CT scan/MRI) and RetCam retina examination; Classified all the patients according to International Intraocular Retinoblastoma Classification, focal therapy were applied in Group A, systemic chemotherapy (CCTV protocol, Cyclosporine, Carboplatin, Teneposide and Vincrinstine) plus operation (include focal therapy, enucleation and orbital exenteration) in Group B,C,D and E; follow-up more than 6 months.

通过回顾性病例分析研究，总结2006年8月至2007年9月本院收治的经过眼部超声、影像学以及眼底照相检查（RetCam婴幼儿眼底照相机）确诊为RB的患儿共66人，按照国际眼内视网膜母细胞瘤分期系统分期，A期选择局部治疗，B、C、D、E期在系统化疗（CCTV方案，环孢霉素、卡铂、替尼泊苷、长春新碱）的基础上，联合手术（包括局部治疗、眼球摘除和眶内容剜出）治疗，随访6个月以上。

Objective To study the method of microwave combined with vincristine for purging leukemic cells in vitro.

目的 研究一种较好的微波和长春新碱的联合方法，用于白血病细胞的体外净化。

MOPP (nitrogen mustard procarbazine prednisone) was the first chemotherapy regimen which produced cures in patients with HD.

MOPP（氮芥、长春新碱、甲基苄肼、强的松）第一个产生的治愈霍奇金淋巴瘤病人的化疗方案。

Objective: To investigate the prepation of the liposome carried with vincristine sulfate and mitoxantrone chlorhydric acid and to evaluate the quality of the liposome.

中文摘要：目的：制备同时包结硫酸长春新碱、盐酸米托蒽醌的复方脂质体并考察其制剂学性质。

Abstract〕 Objective To research the molecular level effects of Fe3O4 nano-magnetic ferrofluid and anticancer drug vincristine on systemic hematogenic immunologic function by means of natural experimental system in vitro.

目的：用体外自然实验体系对纳米级 Fe3O4 磁流体和抗癌药物长春新碱体外对系统血液免疫功能的影响进行分子水平的探讨。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。