长出

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Clinical features of measles in infants and children: time of fever was long, peak temperature of the fever was high, rash onset time long, more complications (especially to the younger infants and children); the main complications were pneumonia and enteritis; one patients died with death rate of 1.12%(1/89); compared the patients treated with antipyretics frequently and less frequently prior to hospitalization, the time of rashing and phase of fever had significant difference between the different treatment before hospitalization (P.05); 50 patients were diagnosed as pneumonia or bronchitis, 40% with reduction in absolute lymphocyte.

结果：本组流动人口占44.94%(40/89)；年龄组以3岁以下的嬰幼儿为主，占71.91%(64/89)，其中8月龄～3岁占56.18%(50/89)；符合麻疹计划免疫接种条件75例，接种率为26.67%(20/75)，流动人口接种率为7.5%(3/40)； 74.39%(61/82)病例发病前均有多家医院就诊史，临床特点：热程长，体温高峰较高，出疹期时间延长，并发症较多；并发症仍以肺炎、肠炎为主；死亡1例，死亡率为1.12%(1/89)；院外未频繁使用退热药组和频繁使用退热药组的出疹期和热程比较有显著性差异，P.05；本组50例诊断为肺炎或支气管炎， 40%淋巴细胞绝对值减少。

Inorganic-organic hybrids were prepared by combining Schiff base complexes of manganese with silicotungstic acid. The composition and structure of the hybrid L^1-Mn-SiW (L^1: N, N'-disalicylidene-1, 6-hexanediamine) were characterized by infrared spectroscopy and thermogravimetry.

将席夫碱锰配合物与硅钨酸结合制备出一种新颖的无机－有机杂化固体催化剂，通过红外光谱和热重表征数据推测出这种催化剂的组成和结构，并将其用于磷酸与等摩尔月桂醇酯化制备长链单烷基磷酸酯。

Kanas lived in a magical elderly, named Emi Gaile, 65-year-old, he was born in Kanas, in the long Kanas, love Kanas, he Kanas Lake Mining The Weicao to produce a "Sool," he can "Sool" Playing a wonderful music.

喀纳斯居住着一位神奇的老人，名叫额尔德什，今年65岁，他生在喀纳斯，长在喀纳斯，深爱着喀纳斯，他用喀纳斯湖边采来的苇草制作出一管&苏尔&，他能用&苏尔&吹奏出美妙的音乐。

Take as a single example this easily overlooked little passage from the rondo of the 23rd piano concerto; it is, in its essence, nothing more than a functional bit of connective tissue taking us from one place to another within the movement's structure. Ten brief bars—about 10 seconds—of basic scalar material, doing its job efficiently and economically. But it is at the same time so richly and amusingly characterized, so full of piping energy, with its determined but struggling piano line climbing its stutteringly inexorable way up to the note E, punctuated along its course by deadpan commentary from the winds, sneaking in the D natural that signals the change of key with almost undetectable sleight of hand—the passage almost constitutes a miniature comic universe all by itself.

请听这段在《第23钢琴协奏曲》中常常被人忽视的回旋曲( http：//img.slate.com/media/87/7_mozart488.asf )，没有哪个功能性连结段能比它更好地仅通过乐章结构就将听众带入另一个世界，长约10秒钟的概要部分(brief bars of basic scalar material)不仅经济有效地发挥其职能，同时又表现出丰富、诙谐的特色，充满木管乐器的活力，伴随着坚决但阻碍不断的钢琴的快速音群移入E大调，其行进过程不时被管乐器部加以修饰、注解，自然而然的转为D大调，转调手法之娴熟几乎无法察觉——这段音乐本身创造出一个微缩的喜剧世界。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

As compared with undrawn PET film, the hot drawn PET film showed shorter crystallization induction period, faster development of long period, anisotropic in SAXS and WAXD patterns, with considerable thermal contraction along the direction of drawing and small lengthening in the direction of drawing during crystallization.

研究结果表明：热拉PET膜的结晶诱导期较短，长周期发展得较快，结晶后小角X-线散射表现出明显的各向异性，在热处理过程中先出现显著的热收缩，随后又表现出结晶伸长现象，这些都和未拉试样有明显的差别。

There is bamboo field between the museum and the road which is on the west , it is connected to the courtyard of the museum by one concrete frame and it separates the mu- seum from the unorderly urban environment .

建筑沿西侧道路后退留出一片长形竹林，这片竹林与建筑本身围合出的方形内庭院空间通过一条透空长廊相互渗透，在竹林与建筑之间的木制步行道，将博物馆建筑与周围混杂的城市环境相对隔离，路过的人可以更深切地感受到博物馆建筑的文化氛围。

Bidentis were studied by adopting indoor bioassay. The results showed that the volatile matter of F. bidentis had not allelopathy to four receptor plants. The eluviated matter inhibited seedling growth of cotton obviously, the inhibition ratio of root and stem were 6.29% and 10.42% respectively. The aqueous extract of litterfall and root exudates had different inhibition to four receptor plants, The synthesis effectof all treatments was significantly greater than eluviated matter treatment except for B3 of the aqueous extract of root exudates, such as the synthesis effect of all treatments (A1, A2, A3, B1, B2, B3 and eluviation) were 44.74, 29.37, 14.5, 31.54, 16.52, 3.55, 1.48 respectively.

结果显示：黄顶菊自然挥发物对受体植物不表现化感作用；黄顶菊淋溶物对棉花幼苗生长表现出明显的抑制作用，对棉花幼苗高度和根长的抑制率分别为6.29%和10.42%；黄顶菊枯落物浸提液和根系分泌物浸提液对4种受体植物均表现出不同程度的抑制作用，除了黄顶菊根系分泌物浸提液 B3处理外，其它处理的综合效应均显著大于淋溶物处理，如黄顶菊枯落物浸提液（ A1、 A2、 A3）、根系分泌物浸提液（ B1、 B2、 B3）以及淋溶物等各处理对受体植物的综合效应分别为44.74、29.37、14.5、31.54、16.52、3.55、1.48。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。