锭

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞，藉羧基荧光素乙酰乙酸染色，用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激，以流式细胞术分析PD98059对淋巴细胞增殖的影响；利用碘化丙锭染色分析PD98059对细胞周期的影响。

The operational result of the system indicates that the temperature raising curves in empty pit, or steel bar loaded pit even the dreggy curves have been greatly improved.

系统的运行结果表明空炉提温曲线、装锭提温曲线和化渣曲线特性具有明显的改善。

Alcan signed a letter of intent with the government in October 2005 to upgrade and expand their Alucam joint venture's Edea aluminium smelter and build a new hydroelectric power station, but has not announced a final decision on whether to go ahead with the project.

加拿大铝业公司于2005年10月份同政府铅锭了意向书，以升级和扩张双方的合资企业--Edea冶炼厂并筹建一个新的水力发电厂，但是双方尚未就是否进行该项目做出最终决定。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带，用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

The movement of the water-cooled mould relative to the ingot is essentia...

同时认为，对于抽锭式重熔过程来讲，结晶器与钢锭之间的相对移动，实际上是接触钢锭表面的渣壳内侧渣璧相对于被拉裂的与结晶器接触的渣壳外侧渣壁的剪切运动。

She improved on the cotton fluffer and successively made the most advanced textile tool of the time, a pedal spinning wheel with three spindles. Combining the advanced textile technique of the Yi ethnic group with the traditional weaving techniques of the Han ethnic group, she also produced textiles such as bedding with various patterns called "Wunijing Bedding".

她改进了弹棉花的弹弓，并成功地制成了当时世界上最先进的纺纱工具——三锭脚踏纺棉车；她还把黎族先进的纺织技术和汉族传统的织造工艺结合在一起，织成了配有各种图案的被褥等物品，被人们称为&乌泥泾被&。

She improved on the cotton fluffer and successively made the most advanced textile tool of the time, a pedal spinning wheel with three spindles. Combining the advanced textile technique of the Li ethnic group with the traditional weaving techniques of the Han ethnic group, she also produced textiles such as bedding with various patterns called "Wunijing Bedding".

她改进了弹棉花的弹弓，并成功地制成了当时世界上最先进的纺纱工具——三锭脚踏纺棉车；她还把黎族先进的纺织技术和汉族传统的织造工艺结合在一起，织成了配有各种图案的被褥等物品，被人们称为&乌泥泾被&。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The results of absorption spectra, KI fluorescence quenching studies, competitive binding studies and thermal denaturation experiments suggested that the interaction between AQS and DNA be intercalative.

在CT DNA存在下，KI对AQS光化学反应产物的荧光猝灭常数K〓比没有CT DNA存在情况下的要小。2.5×10〓mol/L的溴化乙锭即可将3.2×10〓mol/L的AQS从AQS与DNA的结合物中完全置换出来。

Chromosome DNA was purified by isopycnic centrifugation in a CsCl-ethidium bromide.

氯化铯-溴化乙锭梯度离心法纯化DNA。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。