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Among them, the promoter region hypermethylation of cyclin-dependent kinases inhibitors are confined play an important role in cell cycle uncontrol and in CRC carcinogenesis.

近年来普遍认为DNA甲基化与肿瘤抑制基因包括错配修复基因、血管生成抑制基因、细胞粘附分子相关基因的失活有关,是肿瘤发生的第三种机制。

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题,采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

A mispairing PCR-RFLP technique was applied in this study to determine the Insulin-like Growth Factor 2(IGF2) gene intron3 G3072A mutation in an outbred Landrace and Large White, and the gelded boars from Landrace×Large White cross. The difference of corresponding traits and the genetic effects of the boars inherited from parental A allele and inherited from parental G allele were analyzed.

采用错配PCR-RFLP技术鉴别了长白猪和大白猪纯种以及长白×大白猪杂交后代去势公猪胰岛素样生长因子2(IGF2)基因内含子3的3072位点变异,分析了遗传来自父本不同等位基因个体间的相关性状差异及不同等位基因的遗传效应。

Objective Analysis for germline mutation in mismatch repair genes, hMLH1 and hMSH2, in hereditary nonpolyposis colorectal cancer patients and presymptomatic diagnosis in HNPCC families.

目的 通过分析遗传性非息肉性大肠癌患者错配修复基因的遗传性突变,对患者的家族成员进行遗传咨询和症状前的基因诊断。

METHODS: We developed a fully major histocompatibility complex-mismatched murine BMT model without systemic graft-versus-host disease, and challenged mice with aerosolized lipopolysaccharide, a prototypic TLR4 agonist, to determine the effect upon pulmonary alloimmune lung injury.

我们制作了无全身移植物抗宿主反应的全主要组织相容性抗原错配的小鼠BMT模型,并对其采用雾化脂多糖(一种TLR4激动剂)吸入明确同种免疫肺损伤的作用。

Based on the research results of single layer films, three superlattice films of NbN/TiN, TaN/TiN and TaWN/TiN have been designed and synthesized and their microstructure has been investigated. The action of lattice mismatch and elastic modulus difference on the hardness anomalous increase of nano-multilayer films has been compared from the experimental research.

在单层膜的研究基础上,设计并制备了NbN/TiN、TaN/TiN和TaWN/TiN等三种超晶格薄膜,研究了它们的微结构特征,并通过实验研究对比了晶格错配度和弹性模量差异对纳米多层膜硬度异常升高的作用。

It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ.

推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外,还有部分分子在固相合成中发生了消旋作用,需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

Relatively, the intercalation of Λ-〓 into minor groove is more preferable than other intercalating styles.

因此,这些错配碱基对的空间结构和静电势分布也必然影响识别结果。

And we also research the impact of methylating on hydrogen bond and stacked bond in base pairs under considering water molecules.

研究结果表明,烷基化之所以能造成碱基错配是它可使碱基的质子迁移或烯醇互变现象容易发生,改变原有原子的电荷分布或配对模式,同时碱基自身的互变异构体会跟烷基作用,致使互变异构体能够稳定存在,影响正常碱基配对,引起碱基诱变,造成生理疾病的发生。

Moreover,the selectivity of CAs-MB was related to the position and the type of mismatched base.

另外,分子信标的识别能力还与错配碱基的类型及在分子信标中的位点有关。

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