锁状联合

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

In tetrapolarity mating system,A and B factor is two mating types locus locating different chromosome,only when two single spore mycelium form AαAβBαBβ,then can produce to dikaryon and complete whole sexual life history.Study according to the Eger.

在四极性交配系统中，A和B因子分别对应位于不同染色体上的两个交配型位点，两个单核菌丝相遇，只有当它们形成A1A2B1B2或者AαAβBαBβ型时，才能产生具有锁状联合的双核体，完成整个有性生活史。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

摘 要：从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株，将F1子代及亲本(H2，J3)等所有供试单核体菌株的菌丝体两两配对培养，采用核荧光染色观察核相，和光学显微镜观察锁状联合，鉴别菌丝交配反应是否形成双核体菌丝，同时测定了亲本菌株所有F1子代的交配型。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株，将F1子代及亲本(H2，J3)等所有供试单核体菌株的菌丝体两两配对培养，采用核荧光染色观察核相，和光学显微镜观察锁状联合，鉴别菌丝交配反应是否形成双核体菌丝，同时测定了亲本菌株所有F1子代的交配型。

Under microscope, the mycelia were uniform thickness and had clamp connection.

在显微镜下，菌丝粗细均匀，有锁状联合。

According to the diagnostic charater such as the presence or absence of extracellular oxidase of mat, the type of hyphae and its differentiation, the distribution of clamp connection, the color of hyphae and mat and occurrence of spore, the culture character of 60 kinds of polypores in Northeast China were studied.

根据菌落细胞外氧化酶检测反应、菌丝类型及分化、锁状联合分布、菌丝和菌落的颜色、产孢情况等特征，对中国东北针阔叶树60种主要多孔菌，进行了培养特性及鉴定分类系统的研究。

Morphology, and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, myceIia are dikaryocytes with typical clamp connection.

显微形态及核相观察表明：节孢子直径略大于担孢子，担孢子为单核细胞，节孢子绝大多数为单核细胞，少数为双核细胞，菌丝体为双核细胞并具有典型的锁状联合结构。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中，源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落，而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落，暗示担孢子中的两个核具有相同的交配型。

In OWE-SOJ test, a fluffy colony without clampconnection formed in the A=B≠mating interaction where nuclear migration occurs, whilea barrage colony without clamp connection formed in A≠B= interaction where nuclearmigration do not occur. The two colonies could be distinguished clearly from each other.

而在OWE-SOJ试验中，发生核迁移的A=B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B=反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

In OWE-SOJ test, a fluffy colony without clamp connection formed in the A=B≠ mating interaction where nuclear migration occurs, while a barrage colony without clamp connection formed in A≠B= interaction where nuclear migration do not occur. The two colonies could be distinguished clearly each other.

而在OWE-SOJ试验中，发生核迁移的A＝B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B＝反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。