链霉菌

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究，比较了雄蚁和雌蚁的异同，并对可能造成这些差异的行为学联系进行了初步的探讨。

Using Colophony-Paraffin embedding tissue section technique and immunohistochemical streptalidin-peroxidase method, we have investigated the distribution of 5-HT neurons in the brain of Camponotus japonicus, compared the immunoreactivity with GABA, and primarily discussed the character of 5-HT distribution.

本实验采用树脂石蜡组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法，研究了5-HT能神经元在日本弓背蚁大工蚁脑中的分布情况，并与GABA的分布进行了比较，初步探讨了5-HT在各个脑区的分布规律和特点。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

It is expected that hyg would be integrated stablely into the chromosome along with insert while spc/str would be lost during the second cross-over. Gene replacement through these two flanking sequences will result in the susceptibility of the transformants to phage φHAU3, which provides an enrichment for desired transformants by counterselection.

预期在携带有大插入片段的基因置换YAC分子进入野生型变铅青链霉菌后，YAC分子将通过1.4kb或1.0kb片段与染色体发生同源单交换，继而发生同源双交换，hyg连同大插入片段一起稳定地整合到染色体上，而spc／str丢失，从而导致菌株Hyg〓Str〓的表型。

A marine actinomycete strain having antibacterial activity to aquatic pathogens was initially identified as Streptomyces fradiae by cytoderm chemi-component and 16S rDNA sequence analysis.

对一株抗多种水产病原菌的海洋放线菌进行细胞壁化学组分和16S rDNA序列分析，初步鉴定为弗氏链霉菌。

Methods Immunohistochemical streptavidin-peroxidase method was used to detect the expression of MMP-2, MMP-9, TIMP-1, TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles, 55 cases of complete hydatidiform moles, 15 cases of invasive moles and 8 cases of choriocarcinoma.

应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(2周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。

The plasmid DNA of Streptomyces ahygroscopicus was extracted by soda split. The plasmid DNA was partial hydrolyzed by Sau3AI, and 3.5~9 kb DNA fragment was wllected by dialyze bag.

采用碱裂解法从不吸水链霉菌提取染色体DNA，用Sau3AI对染色体DNA进行部分水解，利用透析袋法回收3.5~9kb之间的DNA片段。

Five plasmids for the expression of doxA gene, which were designated as pYG57, pYG502, pYG503, pYG505 and pYG506 were constructed so that the cloned doxA gene were under the control of either promoter of melanin gene or erythromycin resistant gene or their tandem promoters, and a fd terminator downstream.

构建了五个doxA基因链霉菌表达质粒pYG57、pYG502、pYG503、pYG505和pYG506，使得doxA基因在上游的Pmel启动子、红霉素抗性基因启动子或者两者的串联启动子，以及下游的fd终止子控制之下。

Linear and circular plasmids were investigated among the 44 strains of the rice plant endophytic Streptomyces.

以广东番禺和五山地区水稻植株中分离到的内生链霉菌为对象，调查可能存在的内源质粒。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失