链霉菌

In S. erythraea, pZW could express reporter genes thiostrepton resistance gene and enhanced green fluorescin gene. And it is more suitable to express exogenous genes in S. erythraea stably.

在糖多孢红霉菌中，pZW可以表达硫链丝菌肽抗性基因和绿色荧光蛋白基因，是更适合在糖多孢红霉菌中稳定表达外源基因的整合型表达载体。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind Ⅲ. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536.The ligation mixture was transformed into EPI300 competent cells. A total of 5 184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用HindIII部分酶解后回收40kb~60kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

Two outstanding fusants SR-7 and SR-T19 were chose out in the fusion group 90-11×FR-008, the former produces not only antibiotics the parents produced but also a new antibiotic effective gainst a wide range of both gram positive and negative bacteria, which the parents could not produce, and its product of the 37℃ fermentation was very effective against the cotton seedling damping off caused by Rhizoctonia sonali while product of the 26℃ was effective agaist the bacterial rots of turnip and Chinese cabbage; the latter have a much stronger activity against a wide range of fungi and yeasts than the parents.

在90-11×FR-008融合组合中，筛选获得2个优良融合子SR-7和SR-T19，前者除产生亲本产生的抗生素外，还产生亲本不产生的一种新的具有广谱抗细菌活性的碱性水溶性抗生素，防病试验表明，其37℃发酵产物对棉花苗期立枯病具有良好的防治效果，28℃发酵产物对萝卜和大白菜具有明显的防腐作用；后者与亲本FR-008的抗菌谱完全一致，但其活性强度都超过了FR-008；采用RAPD分子标记技术检测了SR-7和SR-T19与亲本的同源性及彼此间的差异，从而首次直接从DNA分子水平上验证了链霉菌的原生质体融合子及其遗传重组的多样性。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

Soil samples were collected from different ecological conditions. More than 70 Streptomyces hygroscopicus strains were isolated from the soil samples. Fermentation centrifugal supernatant of the strains were used as biocontrol agents to bioassay the seedling of rice and barnyard grass and rice pathogens of sheath blightand bakanae. Three strains showed good effects of inhibitingbarnyard grass seedling, the another three strains well inhibited the growth of rice pathogenic fungi.

从国内外不同生态条件下采集的土壤标本中分离到70多个吸水链霉菌，用其发酵离心上清液对水稻、稗草秧苗和稻纹枯病菌、恶苗病菌进行生物测定，结果有3个菌株对稗草根的生长具有较好的抑效果；有3个菌株对水稻病原菌有很好的抑制生长作用。

Normally, the changes of phenotype are closely related to the genome instabilities.

一般地，链霉菌遗传表型的变化与基因组的不稳定密切相关，在10-22中也是如此。

To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated.

在吸水链霉菌分批发酵研究的基础上，通过在菌体生长阶段指数流加葡萄糖，进行高细胞密度培养，获得了较高的菌体量；待菌体生长进入产酶期后，通过补加氮源，为产酶提供充足的氮源，其中通过流加蛋白质氮源，可以减少蛋白酶对成熟MTG的分解，促进产酶。

Chlortetracycline was produced by Streptomyces aureofaciens in 50 liters fermentor.

利用金霉素链霉菌在 5 0L自控发酵罐中发酵生产金霉素。

Capacity ofcarbon flow through HMS regulated the biosynthesis of chlortetracycline to a great extent.

12%的蔗糖、0.5%甘氨酸、溶菌酶酶解lh，是金色链霉菌原生质体制备的较优条件。

The favorite cultural conditions are developed as the following: the initial charge is 15 g in 500 mL Erlenmeyer flask; initial ratio of water to solid is 1.7:1.0 (V/W, rice hull excluding), inoculating quantity is 7 mL, culture temperature is 28℃.

结果表明，链霉菌Men-myco-93-63产孢的适宜条件为：装料量为15 g/500 mL三角瓶，初始水料比为1.7：1.0(V/W，不计稻壳质量)，液体接种量为7 mL，培养温度为28℃。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失