链霉菌

Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点，同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段，并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因，同时也插入了有助于将质粒引入链霉菌的oriT片段，从而构建出了置换整个基因簇的基因置换质粒pHZ691。

According to BLAST results in GenBank, molecular phylogenetic tree (Paup 4.0), and the morphological, physiological and biochemical characters, the strain was preliminarily identified as a Streptomyces.

在GenBank中进行BLAST 相似性分析，利用Paup 4.0 构建系统发育树，并结合形态特征、培养特征及生理生化特征，确定此菌可以划分为链霉菌属，且与嗜热淀粉酶链霉菌S。

In this study rat a-amidase was expressed secretively in S.

本研究在链霉菌中分泌表达了大鼠的α-酰胺化酶，以便利用链霉菌生产酰胺化多肽。

Results of gene disruption indicated that sawC is essential to septation during sporulation. Increase of gene dosage of sawC has no obvious effects on morphological differentiation. When sawC was introduced into Streptomyces coelicolor C13 (whiA13), C13 showed phenotype the same as wild strain.

圈卷产色链霉菌中sawC的破坏导致不能形成孢子分隔。sawC基因剂量的增加对形态分化无明显影响。sawC引入天蓝色链霉菌C13(whiA13)中，可使C13恢复野生型表型。

The main purpose of this work was to obtain the high level expression of hGM-CSF in Streptomyces and study the secretory mechanism of Streptomyces signal peptide.

本实验工作旨在链霉菌中获得高效表达的hGM-CSF，并研究链霉菌信号肽的分泌机制。

The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales.

该链霉菌菌株 Snea253 的分类地位是放线菌门，放线菌纲，放线菌目，链霉菌属，委内瑞拉链霉菌。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索，从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因，构建了doxA基因的链霉菌表达质粒，导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌，并研究了doxA基因在工程菌中的表达，最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质粒的启动子在糖多孢红霉菌中都不能发挥作用，故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子，并利用pSET152质粒上链霉菌染色体整合位点和氨朴霉素抗性基因，构建了表达载体pZMW。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失