链球菌

In order to understand the resistance against common antibiotics in clinic and macrolide antibiotic-resistant mechanism of Streptococcus pneumoniae S.

目的 了解浙江地区肺炎链球菌临床菌株对临床常用抗生素耐药性以及肺炎链球菌对大环内酯类抗生素耐药机制。

Streptococcal pyrogenic exotoxin B is a cysteine protease and carried by every strain of Streptococcus pyogenes. SPE B has been shown to play an important role in invasive group A streptococcal infection.

链球菌致热性外毒素B 是一种cysteine protease，所有A群链球菌都携带致热性外毒素B之基因，在A群链球菌侵入性感染的病程中，致热性外毒素B扮演一个重要的致病角色。

Losing competence might affect their β-lactam resistance, and the consequences were different in different strains.4. To further understanding when and where pneumococci formed transformation in vivo so as to study the influence of pneumococcal transformation to its virulence, we constructed a pneumococcus 22G itstransformation forming could be reported by EGFP.

肺炎链球菌的感受态形成可诱导ciaH和cpoA的表达增加，其感受态缺陷影响到其对β-内酰胺类抗生素的敏感性，但不同菌株影响的结果不同。4、为进一步了解肺炎链球菌在宿主体内转化发生的情况，便于深入研究体内细菌转化对其毒力形成的影响，本研究构建了绿色荧光报告细菌转化发生的肺炎链球菌菌株22G。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

MBC value was 0.61, 0.304, and 0.00475 μgmL^(-1) respectively. Sterilization rate of ceftiofur suspension was the fastest to Actinobacillus, followed by Streptococcus. PAE of ceftiofur suspension was relatively short to E. coli, which illustrated that it was nonconcentration dependent. PAE was obvious longer to Actinobacillus, which showed that it was concentration dependent, however, it revealed part concentration dependent to streptococcus.

头孢噻呋混悬剂对大肠杆菌、放线杆菌及猪兽疫链球菌的MIC值分别为：0.076、0.304、0.00475μgmL^(-1)；MBC值分别为：0.61、0.304、0.00475μgmL^(-1)；头孢噻呋混悬剂对放线杆菌的杀菌速率最快，对链球菌次之；头孢噻呋混悬剂对大肠杆菌PAE较短，呈现非浓度依赖性，对放线杆菌的PAE较长，呈明显的浓度依赖性，而对猪兽疫链球菌呈现部分浓度依赖性。

The specific PCR products of approximately 112bp in length was amplified from the other 10 strains of E.faecalis from lamb naturally infected with E.faecalis,lamb and mouse experimentally infected with E.faecalis by the PCR assay,but no any bands were amplified from Streptococcus equi subsp.equi,Streptococcus G,Streptococcus A,Staphylococcus aureus,Listeria monocytogenes from sheep,Mycoplasma ovipneumoniae and Brucella ovis.

应用该方法对分离的致羔羊脑炎粪肠球菌、由肠球菌导致发病或死亡羔羊脏器以及人工感染死亡小鼠的主要脏器进行扩增，均能得到特异性的DNA条带；对其他7种病原菌，马腺疫链球菌C群、G群链球菌、A群链球菌、金黄色葡萄球菌、羊源李氏杆菌、绵羊布氏杆菌、绵羊肺炎支原体不能扩增出特异的DNA条带。

Results 34 positive cases of Streptococcus, 24 cases were Streptococcus pneumoniae, all showed positive for pneumococcal antigen test.

结果34例链球菌阳性标本中，肺炎链球菌24例，肺炎链球菌抗原试验均为阳性。

Be specific for Streptococcus pneumoniae, were used to identify 49 alpha-hemolytic streptococcal isolates suspected of being pneumococci.

三种PCR检测方法和一个商品化的试剂盒被用于鉴定49株疑似肺炎链球菌的有a溶血的链球菌，这四种方法都基于对16SrRNA基因的杂交，而且都声称对肺炎链球菌有很好的特异性。

For all of GAS toxicity factors, M protein is the most important toxicity factor. M protein is a membrane protein which have α-helical coiled coil structure on the plasma membrane. It helps GAS to attack the host and have resistance to the immune system.

在A型链球菌所有的毒性因子中，M蛋白是最重要的毒性因子。M蛋白是A型链球菌上的一种膜蛋白，在A型链球菌细胞膜上形成α-helical coiled coil结构。M蛋白帮助A型链球菌侵入宿主并且逃避宿主的免疫系统攻击。

Group A Streptococcus is a Human pathogen which causes 18.1 million cases disease and 0.51 million people dies. GAS causes severe disease including scarlet fever, rheumatic fever, rheumatic heart disease and acute glomerulonephritis, the rheumatic heart disease is the major reason which causes heart disease in the children and young people.

A型链球菌是是一种人类的致病菌，在全球每年会有大约1800万人受到A型链球菌的影响，且会有51万人死於A型链球菌所造成的疾病。A型链球菌所造成的疾病包含猩红热、风湿热、风湿性心脏病、急性肾丝球肾炎等，其中风湿性心脏病是造成孩童以及年轻人得到心脏病的主要原因。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。