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链球菌

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In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明,嗜热链球菌在营养丰富的培养基中的生长延滞期缩短,到达对数生长顶点的时间变短,稳定期的时间延长,当经过二次培养后,嗜热链球菌几乎不经过延滞期直接到达对数期,平稳期持续的时间较短,很快到达衰亡期;嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系,菌数多,其代谢旺盛,菌数降低其代谢速度减慢;嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化,并可能存在着细胞凋亡,与菌体增殖和产酸规律密切相关,随着培养时间延长,培养基中营养物质变得贫乏,菌体形态变化和细胞凋亡的情况愈加明显。

Results The positive results were found in all strains of streptococcus gordonii and 66.7% of streptococcus mitis.

结果 所有高登氏链球菌和 66 7%的缓症链球菌与α淀粉酶结合能力为阳性,而血链球菌与口腔链球菌与α唾液淀粉酶结合均为阴性。

Mixed saliva and pooled plaque samples from the smooth surfaces of teeth were collected for microbiologic analysis before administration (baseline~ day 0).. during treatment (days 7.. 14 and 21) and after withdrawal of the agents (days 28~ 42 and 56) and plaque index were recorded. Microbial cultivation were performed by serially diluting the samples with PBS solution and cultivating the aiquots on Mitis Salivarius Agar and MS supplemented with bacitracin for Oral streptococci and Mutans streptococci respectively. The plates were incubated at 37~C under microaerophilic condition(90%N2,10%CO.,) for 48 hot:rs. The number of colony-forming unit per plate was counted and the percentage of Mutans Streptococci per Oral streptococci in dental plaque were determined.

使用前、使用中1、2、3周及停止使用后1、3、5周分别记录菌斑指数并收集唾液及光滑面集合菌斑样本,应用MS及MSB培养基微需氧环境培养检测唾液和菌斑中口腔链球菌属细菌及变形链球菌的菌落形成数,分析各组使用药物前后唾液中变形链球菌的改变及菌斑中变形链球菌在口腔链球菌属细菌中百分比的变化以及变化的延续性。

Besivance is indicated for the treatment of bacterial conjunctivitis in patients aged ≥1 year whose infection is caused by susceptible isolates of the following bacteria: CDC coryneform group G, Corynebacterium pseudodiphtheriticum, Corynebacterium striatum, Haemophilus influenzae, Moraxella lacunata, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Streptococcus mitis group, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus salivarius.

Besivance适用于治疗年龄≥1 岁、由下列细菌的致病分离物所致的细菌性结膜炎患者:CDC棒状杆菌G群,假白喉棒杆菌,纹带棒杆菌,流感嗜血杆菌,腔隙莫拉菌,金黄色葡萄球菌,表皮葡萄球菌,人葡萄球菌,路邓葡萄球菌,缓症链球菌群,口腔链球菌,肺炎链球菌以及唾液链球菌

The most common bacterial cause of acute pharyngitis is Streptococcus pyogenes, or Group A streptococcus, which is referred to as "strep throat."

引起急性咽炎最常见的细菌是化脓性链球菌或者A组链球菌。A组链球菌常引起我们所说的链球菌性咽喉炎。

The results shows that suppurative hemolytic streptococus such as S. pyogenes, S. agalactiae and S. eguisimilis are obviously related to guttate psoriasis, and M6- proteins plays an important role. Other bacteria containing M6- protein liking S. uberis can alsc induce or exaggerate the psoriasis.

研究结果显示,化脓性溶血链球菌中的化脓链球菌、无乳链球菌和似马链球菌与点滴型银屑病的发生有明显相关性,可能主要是菌体中的M6蛋白在起作用,其它含有M6蛋白的细菌如乳房链球菌也可诱发或加重银屑病的发生。

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测,结果,15株链球菌中6株为阳性,其中化脓链球菌3株,无乳链球菌、似马链球菌和乳房链球菌各1株,4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中,在其它链球菌如乳房链球菌中也存在。

Based on Jilin local pig streptothrix popular strains, we have chosen the representative F6 strain to be proceeded completely characteristic authenticates, which can provide consult data for research of manufacture of polyvalent inactivated vaccine.At present, there are reports of pig streptothrix in America, England, France, Russia, Japan, India, Denmark, Canada etc.

目前,美国、英国、法国、俄罗斯、日本、印度、丹麦、荷兰、加拿大等国均有关于猪链球菌分离鉴定及血清型分型的报道,从患败血症和脑炎的猪体内分离的链球菌主要是R群的猪链球菌H型,和S群的猪链球菌I型,只有少数C群、D群和L群引起心内膜炎和败血症的病原菌。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果:1、用PCR方法检测A组链球菌:以A组链球菌致热性外毒素基因speB为靶序列,设计的扩增引物对全部对照菌株的扩增结果为阴性,而全部A组链球菌参考株均能扩增出特异的345bp片段,其中包括三株猩红热链球菌,检测敏感性为6.5pg/μl DNA.2、用PCR方法检测白喉杆菌:以白喉外毒素基因toxB为靶序列,设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段,而全部对照株的扩增结果为阴性,检测敏感性为850fg/μl DNA.3、用PCR方法检测嗜肺军团菌:以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因,设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段,而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Using this model three competence-deficient pneumococci, which was identified by PCR and transformation experiment, were constructed. It is found different strain has different bacterial optimal density for being induced into competence. The optimal density for strains 1, 2 and 22 was 0.1, 0.08and 0.07 respectively. The optimal condition for transformation is c plus y culture at pH8.0. In c plus y culture at pH6.8, which restrained pneumococci forming transformation naturally, pneumococci could be induced to form transformation by CSP.It is concluded that we could induce different S.pneumoniae by CSP into competence in their optimal density in laboratory. 2. It was discussed the relationship between pneumococcal transformation formation and its opportunistic pathogenesis. Comparing their virulence with that of parental strains by challenging mice intraperitoneally and adhering to ECV-304 cell respectively.

结果表明不同肺炎链球菌株的转化最适菌密度不同,1、2和22号菌株的最适转化密度分别为0.1、0.08和0.07;用于转化的CTM培养基最适为pH 8.0的C+Y培养基;在自然转化下不能诱导转化发生的pH 6.8的C+Y培养基中,使用CSP诱导,也能促使细菌发生转化,只是转化效率较低;结论:实验室条件下,不同细菌在一定菌密度时可利用CSP诱导肺炎链球菌成为感受态发生转化。2、为探讨肺炎链球菌转化对毒力表达的影响,本研究分别通过体外粘附人脐静脉血管内皮细胞ECV-304和体内小鼠毒力实验观察1、2和22号肺炎链球菌和其转化缺陷菌株1t、2t和22t毒力的差异。

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