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链激酶

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Immunohistochemical staining indicated that Nattokinase positive staining grains were found in the brush-border membrane of epithelial cell from duodenum, jejunal to ileal in the Nattokinase extract group, and they showed most predominant in the jejunum, and there were many nut-brown positive staining grains in kytoplasm of epithelial cell.

利用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物(Streptavidin-biotin-peroxidase complex,SABC)免疫组织化学技术,在国内外,首次对兔口服摄入的纳豆激酶进行肠道吸收定位研究。

Then they used the pattern formed by the beams as they bounced off the crystals to create computer-generated, 3-D images of the patterns of twisting and folding amino acid chains that make up the different types of pantothenate kinase and their interactions with the other molecules.

然后从晶体的反射光形成的图像出发,通过电脑生成组成不同类型泛酸激酶的折叠扭曲的氨基酸分子链的三维图像以及它们与其他分子相互作用时形成的三维图像。

The pantothenate kinase enzymes consist of two strands of amino acids that fold into various twists and turns to make a complex 3-D structure, White said. These modules, called monomers, snap together to form the enzyme.

每一类型氨基酸都由自己独特的外形和大小,泛酸激酶包含两条氨基酸链,经过折叠扭曲形成复杂的三维结构,这些分子称为单体,彼此咬合形成这个酶。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Although methods for evaluating nonsynonymous coding SNPs are known, several other publicly available computational tools can be utilized to assess polymorphic variants in noncoding regions. As an example, the authors applied multiple methods to select SNPs in DNA double-strand break repair genes.

比如,作者利用多种方法选择DNA双链断裂修复基因SNP,他们利用已有的方法确认了57 个SNP,利用新的方法鉴定了可能影响DNA依赖蛋白激酶催化亚单位的83个SNPs,在这140个SNP中,作者踢出了其中119个预测价值不高的SNP。

To explore the reciprocal effects of host-tumor interaction, we developed a system to assess the gene expression patterns and the diversity of gene expression of ERK and PI3K pathway in CAOV3 human ovarian cancer cells and primary carcinoma-associated fibroblasts cocultured with each other for 48 hours with RT-PCR.

方法1。采用逆转录聚合酶链反应的方法分别检测交互作用48小时后的卵巢癌相关成纤维细胞与卵巢癌细胞中基因表达的改变,CAFs及CAOV3细胞外信号调节激酶通路的ERK,PI3K基因表达的变化;2。

TIP30/CC3 is a member of the short-chain dehydrogenases/reductases family and a serine/threonine kinase.

TIP30/CC3是短链脱氢酶/还原酶家族的一个成员,也是一个丝氨酸/苏氨酸激酶。

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