链体

Cytochrome P450 BM-3 (CYP102) from Bacillus megaterium catalyzes the subterminal hydroxylation of long-chain fatty acids (C12 to C20) at the positions ω-1,ω-2,ω-3 and the epoxidation of unsaturated fatty acids. Artificial mutants of P450 BM-3 also hydroxylate substrates like short-chain fatty acids (C8 to C10), alkanes, cycloalkanes, heteroarenes and polycyclic aromatic hydrocarbons, which offers potential applications in the pharmaceutical industry, environmental measurement and bioremediation and chemical industry.

细胞色素P450BM-3来源于Bacillus megaterium，能催化长链脂肪酸C_(12-C_(20)的次末端(ω-1，ω-2，ω-3)羟化及不饱和脂肪酸的环氧化，其突变体催化的底物拓展到中链的脂肪酸C_8-C_(10、烷烃、环烷烃、杂环烃、多环芳烃等，因此在医药工业、环境检测和修复及化学工业具有广阔的前景。

The unimolecular decomposition and dehydrogenation of ethanol initiate the branched chain reaction, and the 3 isomers of radical C2H5O determine the direction of the reaction chain branching.

乙醇氧化经裂解反应、脱氢反应最终形成支链反应，乙氧基C2H5O的三种同分异构体在链分支中决定了链分支的进行方向。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

This thesis mainly focus on the Enzyme histochemistry and TEM observation of trunk muscle from Branchiostoma belcheri tsingtauense; isolation and recombinant expression of Bbt-myosin heavy chain isoforms; characterization and expression of Bbt-nascent-polypeptide-associated complexαsubunit.In NADH Enzyme histochemistry, two types of muscle lamella can be distinguished .

本论文主要对青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌肉组织进行了酶组织化学实验和电镜观察，分离了青岛文昌鱼肌球蛋白重链基因异构体并将其重组表达；克隆了青岛文昌鱼新生肽链相关复合体α亚基基因，并检测了其时空表达情况。

In the thin films of semicrystalline block copolymers there exist three driving forces: the interaction between substrate and block copolymer, crystallization of crystallizable component and microphase segregation between unlike blocks. The relative strength of these three driving forces can be regulated by various methods.

在这两类结晶性嵌段共聚物薄膜中存在三种作用力：基体和嵌段共聚物之间相互作用力、可结晶链段的结晶以及不同链段之间的排斥作用力。

The excellent characters of the hyperparasitic Streptomyces F46 and the Streptomyces SC1 were tried to integrate into a fusant by protoplast fusion. The study mostly included content as follows: First, the parental strains were identified by two approaches of the classic identify and the molecular taxonomy.

本试验利用原生质体融合技术将重寄生链霉菌F46和链霉菌SC1融合，以期得到整合双亲优良特性于一体的生防菌。

One class is CAAX-mimetic compounds with an imidazolyl residue on one end and a carboxylic group on the other connected by benzodiazepine scaffold at different positions.

连有咪唑基的苯并二氮杂草化合物，以苯并二氮杂草-2-酮为分子骨架，7位连接含有咪唑基的侧链，1位或3位连接含羧基或酯基的侧链。此类化合物模拟了CAAX配体。

When changing the flexible segment -- hydroxyl polyether's type and its molecular weight, and rigid segment -- aromatic isocyanic acid ester's type and function, solid's intensity, the heat resistance, the compressive strength and other performance will also vary.

改变氰凝预聚体结构中的柔性链段——端羟基聚醚的种类和分子量，或调节刚性链段——芳香族异氰酸酯的种类和官能度时，对固结体的强度、耐温性、抗压强度和其它性能可以进行控制。

The application of the genetic codes is another kind of permutable calculation . From DNA chain to mRNA chain , t RNA , ribosome , peptides

密码表应用体系中，从DNA分子到m RNA分子到t RNA，再到核糖体到肽链，没有一步使用了排列数计算方法，从而造成DNA分子信息传递时步步失真，最后变成了肽链具体位置上的氨基酸分子，这里的每一步信息传递都是对密码表的错误运用。

The 43 table of genetic codes is in fact that 64 segments of spacial room in real DNA molecule versus a real molecule of amino acids in protein , which is an obvious logical error .4.Each relevant experiment proved the table of genetic codes has its own permutable pattern from chemical reactants to products , instead of the descriptions in the table of genetic codes .

密码表应用体系中，从DNA分子到m RNA分子到t RNA，再到核糖体到肽链，没有一步使用了排列数计算方法，从而造成DNA分子信息传递时步步失真，最后变成了肽链具体位置上的氨基酸分子，这里的每一步信息传递都是对密码表的错误运用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。