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A modified mutation operator in BCA, Contiguous Region Hypermutation Operator, was introduced; a Markov chain model of the BCA was proposed; a novel method for the construction of transition matrices for the BCA was given. Consequently, it was proved that the BCA was convergent absolutely.

给出了在BCA中使用的一种变异算子-连续区域超体变异算子和BCA的Markov链模型,提出了一个新的构造算法的状态跃迁矩阵的方法,证明了BCA的绝对收敛性。

The distribution of muscle fibers classified on the basis of their content of different myosin heavy chain isoforms was analysed in muscle samples from the digitorum superficialis flexor muscles of postnatal different ages of rats and rabbits by correlating standard actomyosin ATPase and succinic dehydrogenase SDHase histochemistry.

肌纤维的分类建立在它们所含有的不同肌球蛋白重链异构体基础上,对大鼠和家兔生后发育各年龄阶段趾浅屈肌采用标准的肌动球蛋白ATP酶和琥珀酸脱氢酶组织化学染色。

The distribution of muscle fibers classified on the basis of their content of different myosin heavy chain isoforms was analysedin muscle samples fromthe plantaris muscles of postnatal different ages of rats and rabbitsby correlating standard actomyosin ATPase and succinic dehydrogenase SDHase histochemistry.

应用建立在肌球蛋白重链异构体基础上的标准肌动球蛋白ATP酶和琥珀酸脱氢酶组织化学方法,分析大鼠和家兔出生后发育各年龄阶段跖肌纤维型分布。

Polymerase chain reaction ; agglutinin ; pneumonia ; mycoplasma

聚合酶链反应;凝集素;肺炎;支原体属

The study aimed to establish the protoplast transformation system in Alternaria tenuissima.

[目的]建立极细链格孢菌的原生质体遗传转化体系。

Methods The activities of liver microsomal erythromycin N-demethylaseand aminopyrine N-demethylase were measured by spectrophotometry. The levels of mRNA expressions of CYP3A1, CYP1A2, CYP2E1, mdrla and mdrlb in hepatic microsome were determined with RT-PCR method.

用分光光度法测定大鼠肝微粒体红霉素N-脱甲基酶、氨基比林N-脱甲基酶的活性,用逆转录聚合酶链反应技术测定大鼠肝脏CYP3A1、CYP1A2、CYP2E1、mdr la和mdr lb基因的水平。

The effect of crude chitinase on three pathogens showed that it could degrade the cell-wall of thepathogens.The crude chitinase could make Fusarium spp and Alternaria spps cell-wall thin andrupture,cell-wall and bioplasm separate,the bioplasm of Rhizoctonia solani distribute asymmetry andbecome vacuole,the cell-wall thin and rupture also.

几丁质粗酶对三种病原真菌菌丝细胞壁的降解作用显示,几丁质酶可使交链孢和镰刀菌菌丝细胞壁变薄、溶解、质壁分离、原生质膨大;丝核菌原生质体分布不均匀、空泡化、细胞壁变薄并出现断裂现象。

The thiopeptides, by binding within a cleft located between the ribosomal protein L11 and helices 43 and 44 of the 23S rRNA, overlap with the position of domain V of EF-G, thus explaining how this class of drugs perturbs translation factor binding to the ribosome.

核糖体蛋白L11和23SrRNA的43/44螺旋结构形成裂隙,L11的构象变化能够加宽该裂隙从而允许延伸因子EF-G插入,促使GTP水解,为肽链延伸合成过程中核糖体的构象变化提供能量,而Thio能够与L11和23sRNA结合,阻止L11的构象变化,使该裂隙处于闭合状态,抑制EF-G在延伸过程中的作用。

Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae , and then successively incubated with rabbit anti Mycoplasma hyopneumoniae serum and goat anti rabbit IgG biotin to form the solid biotin, which competed with the biotin in the solution for the limited streptavidin horse radish peroxidase conjugate.

酶标板先用猪肺炎支原体包被,再依次用兔抗猪肺炎支原体抗血浆、羊抗兔IgG -生物素温育形成固态生物素,同溶液中的生物素竞争有限量的链霉亲和素 HRP。

To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated.

在吸水链霉菌分批发酵研究的基础上,通过在菌体生长阶段指数流加葡萄糖,进行高细胞密度培养,获得了较高的菌体量;待菌体生长进入产酶期后,通过补加氮源,为产酶提供充足的氮源,其中通过流加蛋白质氮源,可以减少蛋白酶对成熟MTG的分解,促进产酶。

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