铁蛋白

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

In iron-deficient cells, photosynthetic pigments content decreased, efficiency of photosynthesis was low, With the increase of the concentration of the iron-chelator 2, 2'-Dipyridyl in media, Fv/Fm decreased, indicating photoinhibition increased, the results were consistent with previous research; Heterocyst induction had one delayed time dependent on the status of iron-deficiency, More severe of the iron-deficiency condition, more late of the heterocyst induction, It is possible that certain iron-related genes may directly regulate heterocyst development; under different iron limitation conditions, Protein pattern were different, more serious of the iron-deficiency conditions, less bands of the protein pattern, We could not find the iron-deficiency induced protein; In general the toxic effects of ROS were potentiated by excess iron, But our results revealed that even under iron-deficiency conditions, Oxidative damage existed.

铁限制的细胞光合色素含量下降，光合作用能力下降，随着铁螯合剂2，2'-dipyridyl浓度的增加，叶绿素荧光F〓／F〓逐渐降低，显示光抑制变强。与前人的研究结果相似；依赖铁缺失状态，异形胞诱导有一段推迟的时间，铁缺失越严重，诱导越迟，推断某些铁相关的基因会直接调节异形胞发育；在不同铁营养条件下，蛋白质图谱的变化趋势很明显，缺铁越严重，则蛋白质谱带数愈少，但本文未能找到特异诱导蛋白；一般认为铁过量情况下ROS产生加强，本文结果显示即便在铁缺失条件下，也有氧化胁迫伤害，而且缺铁越严重，活性氧伤害越大。

Simultaneously, the total extractible myoglobin decreased and the proportion of metmyoglobin increased significantly when the pressure above 400MPa. Beef discoloration after pressure processing may result from oxidation of ferrous myoglobin to ferric metmyoglobin, globin denaturation and structural changes. At 40℃, the changes of beef color was similar to that of room temperature's. When the temperature rise to 60℃, most of muscle proteins denatured, a small quantity of native myoglobin could not affect the color of beef significantly. Therefore, protein denaturation was attributed to the main cause of discoloration in beef muscle after pressurization at 60℃.

压力处理导致色泽变化的直接原因可能是由于二价铁的肌红蛋白氧化成三价铁的高铁肌红蛋白、球蛋白的变性及结构的变化所致。40℃时的压力处理，色泽的变化规律与室温下相似，只是色泽变化的程度更为剧烈，蛋白质变性在色泽变化中起到的作用逐渐加强。60℃下的压力处理，绝大部分蛋白质发生变性，少量未变性的肌红蛋白对色泽不会产生明显的影响，此温度的压力处理，蛋白质变性是造成肌肉失去红色的主要原因。

One of the potential advantages of the hypoxia-induced factor prolyl hydroxylase inhibitor approach to anemia therapy over ESA is improved regulation of iron absorption and iron recycling to improve the efficiency of iron utilization, partially via a mechanism of hepcidin suppression, to overcome functional iron deficiency.

用作贫血治疗的缺氧诱导因子脯氨酰羟基化酶抑制剂相对于红细胞生成作用刺激剂的一个优势是铁吸收和铁再利用的调节得到改善，提高了铁利用的效率，尤其是通过铁调节蛋白抑制机制，克服功能性铁缺乏。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Methods: In all the SA, blood test, serum ferritin, folacin and vitamin B12 levels and bone marrow cytology examination were done. Then gastroscopy, colonoscopy, liver and kidney function test, hemolytic laboratory indicators, serum thyroid studies and serological markers of viral hepatitis were done as needed.

对140例重度贫血患者均行血常规、铁蛋白、叶酸与维生素B12测定及骨髓细胞学检查，然后根据病情选择性行胃肠镜、肝肾功能、溶血方面实验室检查及甲状腺功能、肝炎标志物、出凝血常规检测等。

Objective To study the influence of nitric oxide in rats with anemia in chronic disease and the effect of NO on the expression of transferrin receptor in bone marrows and to provide experimental evidence for the prevention and treatment of ACD.

目的探讨一氧化氮在慢性病贫血发病中的作用及对骨髓血细胞转铁蛋白受体的影响，为ACD的防治提供实验依据。

Department of Pediatrics, Sichuan Provincial People's Hospital, Chengdu 610072, ChinaOBJECTIVE: To study the influence of nitric oxide in rats with anemia in chronic disease and the effect of NO on the expression of transferrin receptor in bone marrows and to provide experimental evidence for the prevention and treatment of ACD.

目的：探讨一氧化氮在慢性病贫血发病中的作用及对骨髓血细胞转铁蛋白受体的影响，为ACD的防治提供实验依据。

Objective To evaluate the clinical application significance of digestive tract bleeding detection by combined immunoassay of transferrin/hemoglobin and pyramidon chemical method.

目的 评价转铁蛋白/血红蛋白联合免疫法和匹拉米洞化学法在检测消化道出血中的临床应用价值。

CEA in serum and CEA and Ft in gastric juice were determined in 54 of 58 patients with stomach cancer.

本文测定58例胃癌患者的胃液及血清中的癌胚抗原及铁蛋白，并初步探讨其临床意义。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。